Safety and Immunogenicity of Norovirus GI.1/GII.4 Bivalent Virus-Like Particle (VLP) Vaccine in Children

NCT ID: NCT02153112

Last Updated: 2019-04-01

Study Results

Results available

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE2

Total Enrollment

840 participants

Study Classification

INTERVENTIONAL

Study Start Date

2015-06-23

Study Completion Date

2018-06-20

Brief Summary

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The purpose of this study is to select the optimal formulation of the norovirus vaccine from different concentrations of virus-like particles (VLP) combined with aluminum hydroxide for further development in children.

Detailed Description

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The vaccine being tested in this study is called norovirus GI.1/GII.4 bivalent virus-like particle (VLP) vaccine with aluminum hydroxide. The norovirus vaccine is being tested to assess different formulations of the vaccine that will then be further developed. This study will look at the number of antibodies to norovirus formed in children, toddlers and infants who are administered different formulations of the norovirus vaccine.

The study enrolled 840 patients. Participants will be randomly assigned (by chance) to one of ten treatment groups-which will remain undisclosed to the participant and study doctor during the study (unless there is an urgent medical need).

All participants in Cohort 1 will be vaccinated on Day 1 and Day 29 of the study, and all participants in Cohort 2 will be vaccinated on Day 1, Day 56, and Day 112. All treatment groups in Cohort 1 will receive either one dose of the norovirus vaccine, or two doses. One treatment group in Cohort 2 will receive 2 doses of the norovirus vaccine, and the other group will receive 3. In order to keep the treatment undisclosed to the participant and the doctor in Cohort 1, those randomized to the one-dose groups will receive the norovirus vaccine on Day 1, followed by a dose of placebo vaccine on Day 29. In order to keep the treatment arms undisclosed to the participant and the doctor in Cohort 2, those randomized to the two-dose groups will receive the norovirus vaccine on Day 1 and Day 56, followed by a dose of placebo vaccine on Day 112. Placebo vaccine is saline solution. Participants will be asked to record any symptoms that may be related to the vaccine or the injection site in a diary card for 7 days after each vaccination.

This multi-center trial will be conducted in Finland, Panama, and Colombia. The overall time to participate in this study is up to 210 days for participants in Cohort 1 and up to 293 Days for participants in Cohort 2. Participants in Cohort 1 will make 6 visits to the clinic, and participants in Cohort 2 will make 10 visits to the clinic.

Conditions

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Norovirus

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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Cohort 1, Group 1: 1 Dose

Children 4 to \<9 years of age received one dose of either of the 4 formulations (15 µg of GI.1 norovirus VLP and 15 µg GII.4/GI.1/GII.4 (15 μg/50 μg)/GI.1/GII.4 (50 μg/50 μg) or GI.1/GII.4 (50 μg/150 μg) of the norovirus bivalent virus-like particle (VLP) vaccine, intramuscularly (IM) and 500 µg aluminum hydroxide on Day 1, followed by placebo matching norovirus bivalent VLP vaccine IM on Day 29.

Group Type EXPERIMENTAL

GI.1/GII.4 (15/15)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/15 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (15/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/150)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/150 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Placebo

Intervention Type DRUG

Placebo saline solution

Cohort 1, Group 1: 2 Doses

Children 4 to \<9 years of age received 2 doses of either of the 4 formulations (15 µg of GI.1 norovirus VLP and 15 µg GII.4/GI.1/GII.4 (15 μg/50 μg)/GI.1/GII.4 (50 μg/50 μg) or GI.1/GII.4 (50 μg/150 μg) of the norovirus bivalent VLP vaccine and 500 µg aluminum hydroxide IM on Days 1 and 29.

Group Type EXPERIMENTAL

GI.1/GII.4 (15/15)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/15 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (15/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/150)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/150 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Cohort 1, Group 2: 1 Dose

Children 1 to \<4 years of age received one dose of either of the 4 formulations (15 µg of GI.1 norovirus VLP and 15 µg GII.4/GI.1/GII.4 (15 μg/50 μg)/GI.1/GII.4 (50 μg/50 μg) or GI.1/GII.4 (50 μg/150 μg) of the norovirus bivalent VLP vaccine and 500 µg aluminum hydroxide, IM on Day 1, followed by placebo-matching norovirus bivalent VLP vaccine, IM on Day 29.

Group Type EXPERIMENTAL

GI.1/GII.4 (15/15)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/15 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (15/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/150)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/150 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Placebo

Intervention Type DRUG

Placebo saline solution

Cohort 1, Group 2: 2 Doses

Children 1 to \<4 years of age received 2 doses of either of the 4 formulations (15 µg of GI.1 norovirus VLP and 15 µg GII.4/GI.1/GII.4 (15 μg/50 μg)/GI.1/GII.4 (50 μg/50 μg) or GI.1/GII.4 (50 μg/150 μg) of the norovirus bivalent VLP vaccine and 500 µg aluminum hydroxide, IM on Days 1 and 29.

Group Type EXPERIMENTAL

GI.1/GII.4 (15/15)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/15 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (15/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/150)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/150 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Cohort 1, Group 2a: 1 Dose

Children 1 to \<4 years of age received one dose of either of the 4 formulations (15 µg of GI.1 norovirus VLP and 15 µg GII.4/GI.1/GII.4 (15 μg/50 μg)/GI.1/GII.4 (50 μg/50 μg) or GI.1/GII.4 (50 μg/150 μg) of the norovirus bivalent VLP vaccine and 500 µg aluminum hydroxide, IM on Day 1, followed by placebo matching norovirus bivalent VLP vaccine, IM on Day 29.

Group Type EXPERIMENTAL

GI.1/GII.4 (15/15)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/15 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (15/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/150)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/150 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Placebo

Intervention Type DRUG

Placebo saline solution

Cohort 1, Group 2a: 2 Doses

Children 1 to \<4 years of age received 2 doses of either of the 4 formulations (15 µg of GI.1 norovirus VLP and 15 µg GII.4/GI.1/GII.4 (15 μg/50 μg)/GI.1/GII.4 (50 μg/50 μg) or GI.1/GII.4 (50 μg/150 μg) of the norovirus bivalent VLP vaccine and 500 µg aluminum hydroxide, IM on Days 1 and 29.

Group Type EXPERIMENTAL

GI.1/GII.4 (15/15)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/15 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (15/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/150)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/150 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Cohort 1, Group 3: 1 Dose

Toddlers 6 months to \<1 year of age received one dose of either of the 4 formulations (15 µg of GI.1 norovirus VLP and 15 µg GII.4/GI.1/GII.4 (15 μg/50 μg)/GI.1/GII.4 (50 μg/50 μg) or GI.1/GII.4 (50 μg/150 μg) of the norovirus bivalent VLP vaccine and 500 µg aluminum hydroxide, IM on Day 1, followed by placebo matching norovirus bivalent VLP vaccine, IM on Day 29.

Group Type EXPERIMENTAL

GI.1/GII.4 (15/15)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/15 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (15/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/150)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/150 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Placebo

Intervention Type DRUG

Placebo saline solution

Cohort 1, Group 3: 2 Doses

Toddlers 6 months to \<1 year of age will receive 2 doses either of the 4 formulations (15 µg of GI.1 norovirus VLP and 15 µg GII.4/GI.1/GII.4 (15 μg/50 μg)/GI.1/GII.4 (50 μg/50 μg) or GI.1/GII.4 (50 μg/150 μg) of the norovirus bivalent VLP vaccine and 500 µg aluminum hydroxide, IM on Days 1 and 29.

Group Type EXPERIMENTAL

GI.1/GII.4 (15/15)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/15 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (15/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/150)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/150 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Cohort 2, Group 4: 2 Doses

Infants 6 weeks to \<6 months of age received 2 doses of either of the 4 formulations (15 µg of GI.1 norovirus VLP and 15 µg GII.4/GI.1/GII.4 (15 μg/50 μg)/GI.1/GII.4 (50 μg/50 μg) or GI.1/GII.4 (50 μg/150 μg) formulations of the norovirus bivalent VLP vaccine and 500 µg aluminum hydroxide, IM on Days 1 and 56, followed by placebo-matching norovirus bivalent VLP vaccine, IM on Day 112.

Group Type EXPERIMENTAL

GI.1/GII.4 (15/15)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/15 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (15/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/150)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/150 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Placebo

Intervention Type DRUG

Placebo saline solution

Cohort 2, Group 4: 3 Doses

Infants 6 weeks to \<6 months of age received 3 doses of either of the 4 formulations (15 µg of GI.1 norovirus VLP and 15 µg GII.4/GI.1/GII.4 (15 μg/50 μg)/GI.1/GII.4 (50 μg/50 μg) or GI.1/GII.4 (50 μg/150 μg) of the norovirus bivalent VLP vaccine and 500 µg aluminum hydroxide, IM on Days 1, 56 and 112.

Group Type EXPERIMENTAL

GI.1/GII.4 (15/15)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/15 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (15/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (15 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/50)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

GI.1/GII.4 (50/150)

Intervention Type BIOLOGICAL

Norovirus GI.1/GII.4 (50 μg/150 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Interventions

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GI.1/GII.4 (15/15)

Norovirus GI.1/GII.4 (15 μg/15 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Intervention Type BIOLOGICAL

GI.1/GII.4 (15/50)

Norovirus GI.1/GII.4 (15 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Intervention Type BIOLOGICAL

GI.1/GII.4 (50/50)

Norovirus GI.1/GII.4 (50 μg/50 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Intervention Type BIOLOGICAL

GI.1/GII.4 (50/150)

Norovirus GI.1/GII.4 (50 μg/150 μg) bivalent VLP vaccine combined with 500 μg aluminum hydroxide for IM injection

Intervention Type BIOLOGICAL

Placebo

Placebo saline solution

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

1. Male and female participants aged between 6 weeks and less than 9 years at the time of enrollment.
2. Are in good health at the time of entry into the trial as determined by medical history, physical examination (including vital signs) and clinical judgment of the investigator.
3. Participants legally authorized representative (LAR) signs and dates a written, informed consent form (ICF) and any required privacy authorization prior to the initiation of any trial procedures, after the nature of the trial has been explained according to local regulatory requirements. An assent will also be obtained according to age-appropriate country-specific regulations.
4. Participants who can comply with trial procedures and are available for the duration of the trial.

Exclusion Criteria

1. Participants with a clinically significant active infection (as assessed by the investigator) or body temperature 38.0°C (100.4°F) or higher within 3 days of the intended date of vaccination.
2. Have received antipyretic/analgesic medications within 24 hours prior to the intended vaccine administration.
3. Known hypersensitivity or allergy to investigational vaccine (including excipients of the investigational vaccines).
4. Participants with behavioral or cognitive impairment or psychiatric disease that, in the opinion of the investigator, may interfere with the ability to participate in the trial.
5. Has a history of any progressive or severe neurologic disorder, seizure disorder, or neuroinflammatory disease (eg, Guillain-Barré syndrome).
6. Known or suspected impairment/alteration of immune function, including the following:

1. Children \<18 months of age with history of repeated episodes of acute otitis media (AOM) in the first 6 months of life (AOM defined as a bulging tympanic membrane) and not to be confused with otitis media with effusion (OME).
2. Chronic use of oral steroids (equivalent to 20 mg/day prednisone for ≥12 weeks/≥2 mg/kg body weight/day for ≥2 weeks) within 60 days prior to Day 1 (use of inhaled, intranasal, or topical corticosteroids is allowed).
3. Receipt of parenteral steroids (equivalent to 20 mg/day prednisone ≥12 weeks/≥2 mg/kg body weight/day for ≥2 weeks) within 60 days prior to Day 1.
4. Receipt of immunostimulants within 60 days prior to Day 1.
5. Receipt of parenteral, epidural, or intra-articular immunoglobulin preparation, blood products, and/or plasma derivatives within 3 months prior to Day 1 or planned during the full length of the trial.
6. Receipt of immunosuppressive therapy within 6 months prior to Day 1.
7. Human immunodeficiency virus (HIV) infection or HIV-related disease.
8. Chronic Hepatitis B or C infection.
9. Heritable immunodeficiency.
7. Abnormalities of splenic or thymic function.
8. Has a known bleeding diathesis or any condition that may be associated with a prolonged bleeding time.
9. Has any serious chronic or progressive disease according to judgment of the investigator (e.g., neoplasm, insulin dependent diabetes, cardiac, renal, or hepatic disease).
10. Is participating in any clinical trial with another investigational product 30 days prior to first trial visit or intent to participate in another clinical trial at any time during the conduct of this trial.
11. Has received any other vaccines within 14 days (for inactivated vaccines) or 28 days (for live vaccines) prior to enrollment in this trial.
12. Are first degree relatives of individuals involved in trial conduct.
13. Has a history of autoimmune disease.
Minimum Eligible Age

6 Weeks

Maximum Eligible Age

8 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Takeda

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Medical Director Clinical Science

Role: STUDY_DIRECTOR

Takeda

Locations

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Centro de Estudios em Infectologia Pediatrica SAS

Cali, San Fernando, Colombia

Site Status

Espoon Rokotetutkimusklinikka

Espoo, , Finland

Site Status

Etela-Helsingin Rokotetutkimusklinikka

Helsinki, , Finland

Site Status

Ita-Helsingin Rokotetutkimusklinikka

Helsinki, , Finland

Site Status

Jarvenpaan Rokotetutkimusklinikka

Jarvenpaan, , Finland

Site Status

Oulun Rokotetutkimusklinikka

Oulu, , Finland

Site Status

Porin Rokotetutkimusklinikka

Pori, , Finland

Site Status

Seinajoen Rokotetutkimusklinikka

Seinäjoki, , Finland

Site Status

Tampere Vaccine Research Clinic

Tampere, , Finland

Site Status

Turun Rokotetutkimusklinikka

Turku, , Finland

Site Status

CEVAXIN Plaza Carolina - Ciudad de Panama

Panama City, , Panama

Site Status

CEVAXIN

Panama City, , Panama

Site Status

Countries

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Colombia Finland Panama

References

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Lopez P, Lopez-Medina E, Saez-Llorens X, deAntonio R, Masuda T, Mendelman PM, Sherwood J, Baehner F, Borkowski A. Immunogenicity and tolerability of a bivalent virus-like particle norovirus vaccine candidate in children from 6 months up to 4 years of age: A phase 2 randomized, double-blind trial. Hum Vaccin Immunother. 2023 Dec 31;19(1):2204787. doi: 10.1080/21645515.2023.2204787. Epub 2023 May 4.

Reference Type DERIVED
PMID: 37140558 (View on PubMed)

Provided Documents

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Document Type: Study Protocol

View Document

Document Type: Statistical Analysis Plan

View Document

Other Identifiers

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2014-000778-20

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

U1111-1154-9733

Identifier Type: OTHER

Identifier Source: secondary_id

NOR-202

Identifier Type: -

Identifier Source: org_study_id

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