Testing of HIV Protease Inhibitors to Suppress Inflammation and Improve Cardio Pulmonary Hemodynamics in Subjects With Pulmonary Arterial Hypertension

NCT ID: NCT02023450

Last Updated: 2014-09-08

Basic Information

• Recruitment Status: UNKNOWN
• Clinical Phase: EARLY_PHASE1
• Total Enrollment: 20 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2013-12-31
• Study Completion Date: 2015-07-31

Brief Summary

Study Rationale:There is recent evidence that HIV protease inhibitors (HIV-PI) can improve pulmonary hemodynamics in experimental models of pulmonary arterial hypertension (PAH). There is also experimental evidence that both TLR4 and high mobility group box 1 (HMGB1) participate in the pathogenesis of experimental pulmonary hypertension. A recent high throughput screen for inhibitors of HMGB1 induced macrophage activation yielded HIV-protease inhibitors (PIs) as potent inhibitors of HMGB1 induced cytokine production. Based on the experimental evidence we propose a trial to determine whether HIV-PIs will alter the pathobiology of PAH.

Study Objectives:The main objective of this study is to determine whether saquinavir and ritonavir (SQV+RIT) which have a well-characterized safety profile in humans will reduce bio markers of inflammation and pulmonary artery pressures in patients with PAH.

Study Hypothesis:We hypothesize that the HIV-PI, SQV+RIT, will reduce circulating parameters of inflammation including HMGB1, IL1-beta, IL-6, IL-8, IL-10, TNF-alpha and CRP. Our end points will be changes in these parameters from baseline over the duration of the study.We hypothesize that treatment with SQV+RIT will reduce pulmonary artery(PA) pressure of patients with PAH as measured by echocardiography.

Study Design:This is a single center open label phase 0 study to evaluate the effect of SQV +RIT in patients with IPAH. Subjects with IPAH(N=20) will be enrolled into a study, which will be divided into 3 cohorts and entail the administration of HIV protease inhibitors in three doses. The first cohort (n=3) will receive a starting dose of SQV 0.3 mg/kg twice daily in combination with RIT 0.03 mg/kg twice daily. If the first dose is well-tolerated, the second cohort (n= 3 ) with IPAH will be given doses of SQV 3 mg/kg and RIT 0.3 mg/kg twice daily. If the second dose is well-tolerated, the last cohort (n= 14 ) with IPAH will be given doses of SQV 15 mg/kg and RIT 1.5 mg/kg twice daily.

Conditions

Pulmonary Arterial Hypertension

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

micro/low dose saquinavir and ritonavir

To determine if micro dose and low dose SQV+RIT mediates parameters of chronic inflammation in patients with IPAH.

Group Type: EXPERIMENTAL

saquinavir and ritonavir

Intervention Type: DRUG

micro and low dose

standard dose saquinavir and ritonavir

To determine if short-term use of SQV+RIT reduces parameters of chronic inflammation and PA pressure of IPAH based on echocardiographic parameters. Safety issue also evaluated at the same time.

Group Type: EXPERIMENTAL

saquinavir and ritonavir

Intervention Type: DRUG

standard dose

Interventions

saquinavir and ritonavir

micro and low dose

Intervention Type: DRUG

saquinavir and ritonavir

standard dose

Intervention Type: DRUG

Eligibility Criteria

Inclusion Criteria

• Age 18-60
• Idiopathic pulmonary arterial hypertension
• Evidence of a personally signed and dated informed consent document indicating that the subject (or a legally acceptable representative) has been informed of all pertinent aspects of the study
• Had the diagnosis of PAH confirmed by a cardiac catheterization:Mean pulmonary artery pressure (mPAP) ≥ 25 mm Hg (at rest)，a pulmonary capillary wedge pressure equal or less than 15mmHg, and a normal or reduced cardiac output
• Stable PAH therapy for at least 3 months

Exclusion Criteria

• Baseline systemic hypotension, defined as MAP less than 50 mmHg
• Required intravenous inotropes within 30 days prior to study participation
• Has uncontrolled systemic hypertension as evidenced by sitting systolic blood pressure >160 mm Hg or sitting diastolic blood pressure >100 mm Hg at screening
• Has a history of portal hypertension or chronic liver disease, including cirrhosis, chronic alcoholism, hepatitis B and/or hepatitis C (with evidence of recent infection and/or active virus replication) defined as moderate to severe hepatic impairment (Child-Pugh Class B-C)
• Has chronic renal insufficiency as defined by serum creatinine >2.5 mg/dL at screening or requires dialysis support
• Has a hemoglobin concentration <9 g/dL at Screening
• History of atrial septostomy
• Repaired or unrepaired congenital heart disease (CHD)
• Pericardial constriction
• Restrictive or congestive cardiomyopathy
• Left ventricular ejection fraction 40% by multiple gated acquisition scan (MUGA), angiography or echocardiography
• Symptomatic coronary disease with demonstrable ischemia
• Other severe acute or chronic medical or laboratory abnormality that may increase the risk associated with study participation or investigational product administration or may interfere with the interpretation of study results and, in the judgment of the investigator, would make the subject inappropriate for entry into this study
• Has a psychiatric, addictive or other disorder that compromises the ability to give informed consent for participating in this study. This includes subjects with a recent history of abusing alcohol or illicit drugs 30 days prior to study screening Day 1 and for the duration of the study
• Poorly controlled asthma defined by active wheezing and/or cough with FEV1 < 70% predicted, responsive to inhaled BD (>15% increase in FEV1 with BD)
• Clinically significant intercurrent illness (including lower respiratory tract infection) or clinically significant surgery within 4 weeks before the administration of study drug
• History of hypersensitivity or idiosyncratic reaction to drugs from multiple drug classes
• Receipt of an investigational product or device, or participation in a drug research study within a period of 15 days (or 5 half lives of the drug, whichever is longer) before the first dose of study drug
• Blood loss or blood donation >550mL within 90 days or plasma donation >500 mL within 14 days before administration of study drug;
• Patients with a QTc interval > 450 msec
• Has diabetes mellitus as defined by symptoms of hyperglycemia and serum fasting plasma glucose level≥7.0mmol/l or casual plasma glucose≥11.1mmol/l at screen
• Has a hyperlipidemia as TC≥6.22 mmol/L, LDL-C ≥4.14 mmol/L or TG ≥2.26 mmol/L
• History of crohn's disease, ulcerative colitis (UC) and etc. Inflammatory bowel disease (IBD)
• Patients who are not willing to take contraceptive measures during the study
• Patients who are taking certain other medication will need to be evaluated for possible exclusion based on the potential for adverse drug interactions

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 60 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Xiangya Hospital of Central South University

OTHER

Sponsor Role: collaborator

The Third Xiangya Hospital of Central South University

OTHER

Sponsor Role: lead

Responsible Party

Yuan Hong

The Third Xiangya Hospital

Responsibility Role: PRINCIPAL_INVESTIGATOR

Locations

Xiangya hospital

Changsha, Hunan, China

Site Status: RECRUITING

Countries

China

Central Contacts

Li Ying, MD

Role: CONTACT

lydia0312@csu.edu.cn

0086-13787184360

Facility Contacts

YU ZAI XIN, PhD

Role: primary

yuzaixin@126.com

0086-13875873205

References

Li Y, Li XH, Yu ZX, Cai JJ, Billiar TR, Chen AF, Lv B, Chen ZY, Huang ZJ, Yang GP, Song J, Liu B, Yuan H. HIV protease inhibitors in pulmonary hypertension: rationale and design of a pilot trial in idiopathic pulmonary arterial hypertension. Pulm Circ. 2015 Sep;5(3):538-46. doi: 10.1086/682426.

Reference Type: DERIVED

PMID: 26401255 (View on PubMed)

Other Identifiers

HPI-PAH-0

Identifier Type: -

Identifier Source: org_study_id