The ERA Test as a Diagnostic Guide for Personalized Embryo Transfer

NCT ID: NCT01954758

Last Updated: 2019-02-27

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

569 participants

Study Classification

INTERVENTIONAL

Study Start Date

2013-11-25

Study Completion Date

2018-08-31

Brief Summary

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This project seeks to demonstrate the clinical value of the personalised diagnosis of the endometrial factor in infertility.

Detailed Description

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This project seeks to investigate differences in implantation (IR), pregnancy (PR), ongoing pregnancy (OP) rates, miscarriages, deliveries (LB) and obstetrical, delivery and neonatal outcomes among women undergoing IVF treatment with own oocytes, at first site appointment (up to 3 previous implantation failures in other sites) and blastocyst stage (day 5 or 6). Patients are allocated through computer-generated randomization into one of the three groups: Fresh embryo transfer (ET), Frozen embryo transfer (FET) or personalized embryo transfer (pET) after identification of the personalized window of implantation using the endometrial receptivity analysis (ERA) test, all of them following the usual clinical practice in a same-cycle embryo transfer.

A total of 546 infertile women under 38 years old undergoing her first IVF/ICSI cycle with elective blastocyst transfer are randomized in this prospective, multicenter, open label and controlled trial.

Conditions

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Endometrial Receptivity

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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Personalized embryo transfer (pET)

Patients will undergo a cycle of endometrial preparation following hormone replacement therapy (HRT) and an endometrial biopsy in a substituted cycle after 5 days (around 120 hours) of progesterone administration. The ERA test will determine the window of implantation (WOI) for each patient and will recommend the best time for embryo transfer thereby increasing the chances of a successful outcome. In some specific cases (≤ 10%) a second biopsy will be required to help the bioinformatic predictor to ensure the most appropriated moment for the embryo transfer. In a different cycle, patients will undergo a cycle of controlled ovarian stimulation (COS) to obtain oocytes which will be fertilized by IVF/ICSI and vitrified. In a subsequent cycle, following the ERA result, a personalized embryo transfer (pET) will be carried out following the same conditions in which the ERA test was obtained, using 1 or 2 viable blastocysts previously obtained (day 5 or 6 stage)

Group Type EXPERIMENTAL

personalized Embryo Transfer (pET)

Intervention Type OTHER

Frozen embryo transfer (FET)

Patients will undergo a cycle of controlled ovarian stimulation (COS) to obtain oocytes which will be fertilized by IVF/ICSI. In a subsequent cycle, following hormone replacement therapy (HRT), a frozen embryo transfer (FET) will be performed using 1 or 2 viable blastocysts previously obtained (day 5 or 6 stage).

Group Type ACTIVE_COMPARATOR

Frozen Embryo Transfer (FET)

Intervention Type OTHER

Fresh embryo transfer (ET)

Patients will undergo a controlled ovarian stimulation cycle (COS) to obtain oocytes which will be fertilized by IVF/ICSI. In the same cycle, a fresh embryo transfer will be performed using 1 or 2 viable blastocysts previously obtained (day 5 or 6 stage).

Group Type ACTIVE_COMPARATOR

Fresh Embryo Transfer (ET)

Intervention Type OTHER

Interventions

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personalized Embryo Transfer (pET)

Intervention Type OTHER

Frozen Embryo Transfer (FET)

Intervention Type OTHER

Fresh Embryo Transfer (ET)

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

1. Patients indicated to undergo a cycle of IVF/ICSI with their own oocytes.
2. Age ≤ 37 years
3. BMI: 18.5 to 30
4. Normal ovarian reserve (AFC ≥ 8; FSH \< 8)
5. The most appropriated stimulation protocol will be decided by their doctor.
6. Blastocyst transfer (on day 5 or 6)
7. Blastocyst vitrification with open protocols (Cryotop, Cryoleaf, or Cryolock) or closed protocols (Cryotip or CBSStraw.)
8. Any pathology affecting the endometrial cavity such as polyps/sub-mucosal myomas, intramural myomas \> 4 cm, or hydrosalpinx affecting the endometrial cavity must be previously operated.

Exclusion Criteria

1. Patients with recurrent miscarriages (\> 2 previous biochemical pregnancies or \> 2 spontaneous miscarriages)
2. Patients with a severe male factor (spermatozoa \< 2 million/ml)
3. Patients with implantation failure (\>3 failed cycles with good quality embryos)


1. Endogenous progesterone level ≥ 1,5 ng/ml at the day of hCG administration in all groups.
2. Absence of blastocysts (day 5 or 6) for embryo transfer.
3. Risk of ovarian hyperstimulation syndrome in any of the three groups and therefore a clinical indication to cancel the transfer cycle where the stimulated patient is from group A (ET).
Minimum Eligible Age

18 Years

Maximum Eligible Age

37 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Igenomix

INDUSTRY

Sponsor Role lead

Responsible Party

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Carlos Simon

Scientific Director IGENOMIX; Gynaecologist IVI Valencia

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Carlos Simon, MDPhD

Role: PRINCIPAL_INVESTIGATOR

IVI Valencia / Igenomix

Locations

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Centre of Reproductive Medicine UZ Brussles

Brussels, , Belgium

Site Status

Centro de Infertilidade e Medicina Fetal do Norte Fluminence

Campos dos Goytacazes, Rio de Janeiro, Brazil

Site Status

Centro de Reproduçao Humana Nilo Frantz

Porto Alegre, Rio Grande do Sul, Brazil

Site Status

Centro de Reprodução Governador Mario Covas

São Paulo, , Brazil

Site Status

Sofia Hospital of Reproductive Medicine - SBALAGRM

Sofia, , Bulgaria

Site Status

Oak Clinic Sumiyoshi

Osaka, , Japan

Site Status

IVI Panama

Panama City, , Panama

Site Status

IVI Bilbao

Leioa, Bizkaia, Spain

Site Status

IVI Madrid

Aravaca, Madrid, Spain

Site Status

IVI Vigo

Vigo, Pontevedra, Spain

Site Status

IVI Alicante

Alicante, , Spain

Site Status

IVI Barcelona

Barcelona, , Spain

Site Status

ProcreaTec

Madrid, , Spain

Site Status

IVI Sevilla

Seville, , Spain

Site Status

IVI Valencia

Valencia, , Spain

Site Status

Bahceci Health Group

Istanbul, , Turkey (Türkiye)

Site Status

Countries

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Belgium Brazil Bulgaria Japan Panama Spain Turkey (Türkiye)

References

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Katzorke N, Vilella F, Ruiz M, Krussel JS, Simon C. Diagnosis of Endometrial-Factor Infertility: Current Approaches and New Avenues for Research. Geburtshilfe Frauenheilkd. 2016 Jun;76(6):699-703. doi: 10.1055/s-0042-103752.

Reference Type DERIVED
PMID: 27365540 (View on PubMed)

Other Identifiers

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1304-C-107-CS

Identifier Type: -

Identifier Source: org_study_id

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