Increased Activity of a Renal Salt Transporter (ENaC) in Diabetic Kidney Disease

NCT ID: NCT01918488

Last Updated: 2013-10-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

80 participants

Study Classification

INTERVENTIONAL

Study Start Date

2013-10-31

Brief Summary

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The purpose of the study is to determine whether a diuretic drug called amiloride is capable of increasing renal salt excretion and thereby decrease blood pressure in diabetic patients with kidney disease. Our hypothesis states that amiloride is capable of reducing blood pressure in these patients and thus decrease the cardiovascular risk associated with diabetic kidney disease.

Detailed Description

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Conditions

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Diabetic Nephropathies Hypertension

Keywords

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Diabetic Nephropathies Hypertension Proteinuria Albuminuria Epithelial Sodium Channels Epithelial Sodium Channel Blockers Amiloride

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Nephropathy

Diabetics with diabetic nephropathy receiving first a standardized salt diet (200 mmol NaCl/day) for 4 days and then amiloride tablet 20 mg two times daily (morning and afternoon) for 2 days.

Group Type EXPERIMENTAL

Standardized salt diet

Intervention Type DIETARY_SUPPLEMENT

200 mmol NaCl per day given as three meals daily for 4 consecutive days.

Amiloride

Intervention Type DRUG

Amiloride tablet 20 mg two times daily (morning and afternoon) for two consecutive days.

Control

Diabetics without nephropathy receiving a standardized salt diet (200 mmol NaCl/day) for 4 days, then amiloride tablet 20 mg two times daily (morning and afternoon) for 2 days.

Group Type EXPERIMENTAL

Standardized salt diet

Intervention Type DIETARY_SUPPLEMENT

200 mmol NaCl per day given as three meals daily for 4 consecutive days.

Amiloride

Intervention Type DRUG

Amiloride tablet 20 mg two times daily (morning and afternoon) for two consecutive days.

Interventions

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Standardized salt diet

200 mmol NaCl per day given as three meals daily for 4 consecutive days.

Intervention Type DIETARY_SUPPLEMENT

Amiloride

Amiloride tablet 20 mg two times daily (morning and afternoon) for two consecutive days.

Intervention Type DRUG

Other Intervention Names

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Triamterene

Eligibility Criteria

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Inclusion Criteria

* Type 1 diabetes
* Negative pregnancy test at inclusion and taking contraceptive medication
* One group with diabetic nephropathy and overt proteinuria
* One normoalbuminuric group without nephropathy
* Creatinine clearance \> 40 ml/min

Exclusion Criteria

* Type 2 diabetes
* Receiving amiloride, glucocorticoids, aldosterone or spironolactone
* Clinically relevant organic or systemic disease including malignancy
Minimum Eligible Age

18 Years

Maximum Eligible Age

90 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Odense University Hospital

OTHER

Sponsor Role collaborator

Region of Southern Denmark

OTHER

Sponsor Role collaborator

The Ministry of Science, Technology and Innovation, Denmark

OTHER_GOV

Sponsor Role collaborator

Danish Heart Foundation

OTHER

Sponsor Role collaborator

University of Southern Denmark

OTHER

Sponsor Role lead

Responsible Party

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Henrik Andersen, MD

MD, PhD student

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Henrik Andersen, MD

Role: PRINCIPAL_INVESTIGATOR

University of Southern Denmark

Jan Erik Henriksen, MD, PhD

Role: STUDY_DIRECTOR

Odense University Hospital

Claus Bistrup, MD, PhD

Role: STUDY_DIRECTOR

Odense University Hospital

Boye L Jensen, MD, PhD

Role: STUDY_DIRECTOR

University of Southern Denmark

Locations

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Cardiovascular and Renal Research

Odense, , Denmark

Site Status

Countries

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Denmark

References

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Svenningsen P, Bistrup C, Friis UG, Bertog M, Haerteis S, Krueger B, Stubbe J, Jensen ON, Thiesson HC, Uhrenholt TR, Jespersen B, Jensen BL, Korbmacher C, Skott O. Plasmin in nephrotic urine activates the epithelial sodium channel. J Am Soc Nephrol. 2009 Feb;20(2):299-310. doi: 10.1681/ASN.2008040364. Epub 2008 Dec 10.

Reference Type BACKGROUND
PMID: 19073825 (View on PubMed)

Svenningsen P, Uhrenholt TR, Palarasah Y, Skjodt K, Jensen BL, Skott O. Prostasin-dependent activation of epithelial Na+ channels by low plasmin concentrations. Am J Physiol Regul Integr Comp Physiol. 2009 Dec;297(6):R1733-41. doi: 10.1152/ajpregu.00321.2009. Epub 2009 Sep 30.

Reference Type BACKGROUND
PMID: 19793956 (View on PubMed)

Saha C, Eckert GJ, Ambrosius WT, Chun TY, Wagner MA, Zhao Q, Pratt JH. Improvement in blood pressure with inhibition of the epithelial sodium channel in blacks with hypertension. Hypertension. 2005 Sep;46(3):481-7. doi: 10.1161/01.HYP.0000179582.42830.1d. Epub 2005 Aug 22.

Reference Type BACKGROUND
PMID: 16116042 (View on PubMed)

Buhl KB, Friis UG, Svenningsen P, Gulaveerasingam A, Ovesen P, Frederiksen-Moller B, Jespersen B, Bistrup C, Jensen BL. Urinary plasmin activates collecting duct ENaC current in preeclampsia. Hypertension. 2012 Nov;60(5):1346-51. doi: 10.1161/HYPERTENSIONAHA.112.198879. Epub 2012 Sep 17.

Reference Type BACKGROUND
PMID: 22987920 (View on PubMed)

Isaksson GL, Hinrichs GR, Andersen H, Bach ML, Weyer K, Zachar R, Henriksen JE, Madsen K, Lund IK, Mollet G, Bistrup C, Birn H, Jensen BL, Palarasah Y. Amiloride Reduces Urokinase/Plasminogen-Driven Intratubular Complement Activation in Glomerular Proteinuria. J Am Soc Nephrol. 2024 Apr 1;35(4):410-425. doi: 10.1681/ASN.0000000000000312. Epub 2024 Jan 23.

Reference Type DERIVED
PMID: 38254266 (View on PubMed)

Other Identifiers

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13-04-R94-A4513-22770

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

2013-052

Identifier Type: -

Identifier Source: org_study_id