A Study to Evaluate the Safety, Pharmacokinetics and Pharmacodynamics of N-Rephasin® SAL200 in Healthy Male Volunteers

NCT ID: NCT01855048

Last Updated: 2021-11-03

Study Results

Results available

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

36 participants

Study Classification

INTERVENTIONAL

Study Start Date

2013-08-06

Study Completion Date

2014-02-07

Brief Summary

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The Objectives of this study is to evaluate the safety, pharmacokinetics and pharmacodynamics of single dose of N-Rephasin® SAL200 in healthy male subjects.

Detailed Description

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The purposes of this study are to evaluate the pharmacokinetics, and pharmacodynamics and safety of an experimental intravenous medication, N-Rephasin® SAL200 in healthy male. Participants will include 36 male volunteers. Participants will be grouped according to dosage of N-Rephasin® SAL200 including placebo (0 mg/kg). Study procedures include: check of vital signs, reporting any experienced side effects, physical examination including assessment of the cardiovascular system, and blood sample collection for monitoring of pharmacokinetics, pharmacodynamics and antibody production etc. Participants will be involved in study related procedures for up to 50 days after injection.

Conditions

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Healthy Volunteers Anti-Bacterial Agents Methicillin-Resistant Staphylococcus Aureus

Keywords

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N-Rephasin® SAL200 endolysin Placebo

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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N-Rephasin® SAL200

N-Rephasin® SAL200, 0.1 mg/kg, 0.3 mg/kg, 1 mg/kg, 3 mg/kg, 10 mg/kg

Group Type EXPERIMENTAL

N-Rephasin® SAL200

Intervention Type BIOLOGICAL

continuous intravenous infusion over 60 minutes

INT200-Placebo

Placebo

Group Type PLACEBO_COMPARATOR

INT200-Placebo

Intervention Type OTHER

Formulation buffer for continuous intravenous infusion over 60 minutes

Interventions

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N-Rephasin® SAL200

continuous intravenous infusion over 60 minutes

Intervention Type BIOLOGICAL

INT200-Placebo

Formulation buffer for continuous intravenous infusion over 60 minutes

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Healthy male subject whose age is 20 \~ 45 at the time of screening visit.
* Body weight of ≥50kg and \<90kg, while within ±20% of the ideal body weight. \[ideal body weight(kg) = {height(cm)-100}x 0.9\]
* Subject agreed to participate in the trial and to follow all of trial-related rules with a full understanding, after having a full account of the trial

Exclusion Criteria

* Present disease(s) or medical history(ies) which is(are) clinically significant on liver, heart, nervous system, respiratory system, haemato-oncology, cardiovascular or psychopathy.
* Diagnosed or suspected infectious disease within 30 days in prior to the administration.
* Clinically significantly allergic to drug(s) containing AI of N-Rephasin® SAL200 or to other drugs including aspirin and antibiotics, or has medical history(ies) on such allergy.
* Already has taken other drug(s) containing AI of N-Rephasin® SAL200.
* Positive for Antibody of N-Rephasin® SAL200.
* SBP≤90mmHg or DBP≤50mmHg, otherwise, SBP≥150mmHg or DBP≥100mmHg in Vital sign which was measured after taking 3 minutes of resting in sitting position.
* Has medical history of drug abuse or positive to drug abuse in urine drug screening.
* Has taken any prescription drug(s) or herbal medicine(s) within 14 days prior to the administration, otherwise, any OTC(Over the counter) (s) or vitamin(s) within 7 days prior to the administration(However, can participate in the study if investigator makes a decision that the subject can participate regardless the drug taken).
* Has taken any other study drugs within 2 months prior to the administration.
* Has donated blood(whole blood donation or component transfusion) within (2 months or 1 month, respectively) in prior to the administration, otherwise, has received blood transfusion within 1 month in prior to the administration.
* Smoke at present or positive to metabolism of nicotine in urine test.
* Drink regularly(over 21 units/week, 1 unit= 10 g of pure alcohol), otherwise, is not able to interrupt drinking and smoking in study period.
* Investigator made a decision that the subject is not eligible based on results of laboratory test or other reason.
* Not agree with contraception for 60days after the administration, otherwise, notification of pregnancy in case of his partner is pregnant for 90 days after the administration.
Minimum Eligible Age

20 Years

Maximum Eligible Age

45 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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Intron Biotechnology, Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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In Jin Jang, M.D., Ph. D.

Role: PRINCIPAL_INVESTIGATOR

Seoul National University Hospital

Locations

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Seoul National University Hospital

Seoul, , South Korea

Site Status

Countries

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South Korea

References

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Loessner MJ. Bacteriophage endolysins--current state of research and applications. Curr Opin Microbiol. 2005 Aug;8(4):480-7. doi: 10.1016/j.mib.2005.06.002.

Reference Type RESULT
PMID: 15979390 (View on PubMed)

Fischetti VA. Bacteriophage lysins as effective antibacterials. Curr Opin Microbiol. 2008 Oct;11(5):393-400. doi: 10.1016/j.mib.2008.09.012. Epub 2008 Oct 14.

Reference Type RESULT
PMID: 18824123 (View on PubMed)

Loeffler JM, Nelson D, Fischetti VA. Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase. Science. 2001 Dec 7;294(5549):2170-2. doi: 10.1126/science.1066869.

Reference Type RESULT
PMID: 11739958 (View on PubMed)

Wu JA, Kusuma C, Mond JJ, Kokai-Kun JF. Lysostaphin disrupts Staphylococcus aureus and Staphylococcus epidermidis biofilms on artificial surfaces. Antimicrob Agents Chemother. 2003 Nov;47(11):3407-14. doi: 10.1128/AAC.47.11.3407-3414.2003.

Reference Type RESULT
PMID: 14576095 (View on PubMed)

Schellekens H. Immunogenicity of therapeutic proteins. Nephrol Dial Transplant. 2003 Jul;18(7):1257-9. doi: 10.1093/ndt/gfg164. No abstract available.

Reference Type RESULT
PMID: 12808158 (View on PubMed)

Jun SY, Jang IJ, Yoon S, Jang K, Yu KS, Cho JY, Seong MW, Jung GM, Yoon SJ, Kang SH. Pharmacokinetics and Tolerance of the Phage Endolysin-Based Candidate Drug SAL200 after a Single Intravenous Administration among Healthy Volunteers. Antimicrob Agents Chemother. 2017 May 24;61(6):e02629-16. doi: 10.1128/AAC.02629-16. Print 2017 Jun.

Reference Type DERIVED
PMID: 28348152 (View on PubMed)

Related Links

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https://www.semanticscholar.org/paper/Guidance-for-Industry-Estimating-the-Maximum-Safe-Alert/8750b5d4c6564d44ac84826e3760f15ea0b836de

FDA, Guidance for Industry, Estimating the Maximum Safe Starting Dose inInitial Clinical Trials for Therapeutics in Adult Healthy Volunteers, 2005.

Other Identifiers

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SAL200-1A

Identifier Type: -

Identifier Source: org_study_id