Evaluation of Bacillus Subtilis R0179 in Healthy Young Adults

NCT ID: NCT01802151

Last Updated: 2015-12-17

Study Results

Results available

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

83 participants

Study Classification

INTERVENTIONAL

Study Start Date

2012-07-31

Study Completion Date

2012-10-31

Brief Summary

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The purpose of this study is to evaluate the effect of three doses of the probiotic B. subtilis (Bacillus subtilis R0179) delivered in capsules on gastrointestinal and general wellness in healthy young adults, survival through the gastrointestinal tract and impact on intestinal microbes.

Detailed Description

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A 6 week randomized, double blind placebo controlled trial in healthy young adults aged 18 to 50. Participants will be administered an oral dose of B. subtilis or placebo (1 capsule/day) for a period of 4 weeks. Viable counts will be measured from stool samples to assess transit survival, daily questionnaires (DQ) will be administered to assess general wellness, and gastrointestinal (GI) symptoms will be evaluated three times with the Gastrointestinal Symptom Response Scale (GSRS) questionnaire.

Conditions

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Healthy

Keywords

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probiotics microbiota survival gastrointestinal wellness

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

TRIPLE

Participants Investigators Outcome Assessors

Study Groups

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Placebo

Placebo (starch, magnesium stearate, citric acid) capsules once daily for 4 weeks.

Group Type PLACEBO_COMPARATOR

Placebo

Intervention Type DIETARY_SUPPLEMENT

Placebo (starch, magnesium stearate, citric acid) capsules for a period of 4 weeks. One capsule per day.

B. subtilis R0179 (10 billion CFU)

B. subtilis R0179 (approximately 10 billion CFU/capsule) once daily for 4 weeks.

\* CFU (Colony Forming Unit)

Group Type EXPERIMENTAL

B. subtilis R0179

Intervention Type DIETARY_SUPPLEMENT

B. subtilis R0179 for a period of 4 weeks. One capsule per day.

B. subtilis R0179 (1 billion CFU)

B. subtilis R0179 (approximately 1 billion CFU/capsule) once daily for 4 weeks.

Group Type EXPERIMENTAL

B. subtilis R0179

Intervention Type DIETARY_SUPPLEMENT

B. subtilis R0179 for a period of 4 weeks. One capsule per day.

B. subtilis R0179 (0.1 billion CFU)

B. subtilis R0179 (approximately 0.1 billion CFU/capsule) once daily for 4 weeks.

Group Type EXPERIMENTAL

B. subtilis R0179

Intervention Type DIETARY_SUPPLEMENT

B. subtilis R0179 for a period of 4 weeks. One capsule per day.

Interventions

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B. subtilis R0179

B. subtilis R0179 for a period of 4 weeks. One capsule per day.

Intervention Type DIETARY_SUPPLEMENT

Placebo

Placebo (starch, magnesium stearate, citric acid) capsules for a period of 4 weeks. One capsule per day.

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* Are 18-50 years of age
* Are willing and able to complete the Informed Consent Form in English
* Are willing to complete the GPAQ (Global Physical Activity Questionnaire) at baseline and during the last week of the study as well as completing the GSRS (Gastrointestinal Symptom Response Survey) questionnaire at baseline, during treatment and post treatment
* Are currently "moderately active" as determined by the GPAQ (Global Physical Activity Questionnaire)
* Are willing to have height and weight measured and to provide demographic information (age, race, sex)
* Are willing to consume B. subtilis R0179 (approximate doses of 1, 10, or 0.1 billion CFU per day) or placebo for a 28 day period by capsule (1 per day)
* Are willing to complete daily questionnaires regarding general and gastrointestinal wellness for the duration of the study
* Are willing to provide 1 stool at baseline, 1 stool during week 4 of the treatment and 1 stool after 7 days of washout
* Are willing to provide a social security number to receive study payment. Note: the subject can still participate if unwilling to provide social security number, but no financial reimbursement can be provided
* Are willing to have internet access for the duration of the study

* Are currently taking medications for constipation or diarrhea
* Have taken antibiotics within the past four weeks prior to randomization
* Are currently taking probiotics supplements and do not want to discontinue a minimum of two weeks prior to the study
* Known to have or are currently being treated for any diseases or illnesses such as gastrointestinal disease (gastric ulcers, Crohn's, ulcerative colitis, etc.), other chronic diseases (diabetes, kidney disease, etc.) or immune-compromising diseases or conditions (HIV, AIDS, autoimmune, hepatitis, cancer, transplant patient etc.)
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Lallemand Human Nutrition

UNKNOWN

Sponsor Role collaborator

University of Florida

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Wendy J Dahl, PhD, RD

Role: PRINCIPAL_INVESTIGATOR

University of Florida

Bobbi Henken-Langkamp, PhD, RD

Role: PRINCIPAL_INVESTIGATOR

University of Florida

Volker Mai, PhD

Role: PRINCIPAL_INVESTIGATOR

University of Florida

Locations

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University of Florida

Gainesville, Florida, United States

Site Status

Countries

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United States

References

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Hong HA, Duc le H, Cutting SM. The use of bacterial spore formers as probiotics. FEMS Microbiol Rev. 2005 Sep;29(4):813-35. doi: 10.1016/j.femsre.2004.12.001. Epub 2004 Dec 16.

Reference Type BACKGROUND
PMID: 16102604 (View on PubMed)

Oelschlaeger TA. Mechanisms of probiotic actions - A review. Int J Med Microbiol. 2010 Jan;300(1):57-62. doi: 10.1016/j.ijmm.2009.08.005. Epub 2009 Sep 23.

Reference Type BACKGROUND
PMID: 19783474 (View on PubMed)

Sanders ME, Morelli L, Tompkins TA. Sporeformers as Human Probiotics: Bacillus, Sporolactobacillus, and Brevibacillus. Compr Rev Food Sci Food Saf. 2003 Jul;2(3):101-110. doi: 10.1111/j.1541-4337.2003.tb00017.x.

Reference Type BACKGROUND
PMID: 33451235 (View on PubMed)

Tompkins TA, Hagen KE, Wallace TD, Fillion-Forte V. Safety evaluation of two bacterial strains used in Asian probiotic products. Can J Microbiol. 2008 May;54(5):391-400. doi: 10.1139/w08-022.

Reference Type BACKGROUND
PMID: 18449224 (View on PubMed)

Tompkins TA, Xu X, Ahmarani J. A comprehensive review of post-market clinical studies performed in adults with an Asian probiotic formulation. Benef Microbes. 2010 Mar;1(1):93-106. doi: 10.3920/BM2008.1005.

Reference Type BACKGROUND
PMID: 21840798 (View on PubMed)

Hanifi A, Culpepper T, Mai V, Anand A, Ford AL, Ukhanova M, Christman M, Tompkins TA, Dahl WJ. Evaluation of Bacillus subtilis R0179 on gastrointestinal viability and general wellness: a randomised, double-blind, placebo-controlled trial in healthy adults. Benef Microbes. 2015 Mar;6(1):19-27. doi: 10.3920/BM2014.0031.

Reference Type DERIVED
PMID: 25062611 (View on PubMed)

Related Links

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http://www.ncbi.nlm.nih.gov/pubmed/25062611

Evaluation of Bacillus subtilis R0179 on gastrointestinal viability and general wellness: a randomised, double-blind, placebo-controlled trial in healthy adults.

Other Identifiers

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177-2012

Identifier Type: -

Identifier Source: org_study_id