Obesity-related Genes in Taiwanese Undergoing Weight Loss

NCT ID: NCT01684280

Last Updated: 2012-09-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

20 participants

Study Classification

OBSERVATIONAL

Study Start Date

2010-06-30

Study Completion Date

2010-09-30

Brief Summary

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To investigate:

1. Associations between miRNA and insulin signaling-related gene expressions in abdominal adipose tissues in obese subjects.
2. Differences in miRNAs expressed by intrabdominal omental adipose tissues between genders.

Detailed Description

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Compared the differences in microRNA expressions of abdominal adipose tissue in morbid obese male and female subjects to elucidate the possible regulatory role of microRNA in insulin signaling-related gene expression levels and its association with biochemical markers. We hope to find a miRNA that can be used to predict the syndrome associated with obesity.

Conditions

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Morbid Obesity

Keywords

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abdominal adipose tissue; microRNA

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

CROSS_SECTIONAL

Study Groups

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gender

Paired samples of abdominal subcutaneous and intrabdominal omental adipose tissue were obtained from men and women who underwent bariatric surgery.

gender

Intervention Type GENETIC

Paired samples of abdominal subcutaneous and intrabdominal omental adipose tissue were obtained from men and women who underwent bariatric surgery.

Interventions

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gender

Paired samples of abdominal subcutaneous and intrabdominal omental adipose tissue were obtained from men and women who underwent bariatric surgery.

Intervention Type GENETIC

Other Intervention Names

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BMI were all over 40 kg/ m2

Eligibility Criteria

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Inclusion Criteria

* Morbid obesity,BMIs\> 40 kg/m2
* Subjects who underwent bariatric surgery

Exclusion Criteria

* Obese subjects with complicated metabolic disorders
* Thyroid disease
Minimum Eligible Age

19 Years

Maximum Eligible Age

63 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Science and Technology Council, Taiwan

OTHER_GOV

Sponsor Role collaborator

Sung Ling Yeh

OTHER

Sponsor Role lead

Responsible Party

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Sung Ling Yeh

professor

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Wang Weu

Role: STUDY_DIRECTOR

Comprehensive Weight Management Center Taipei Medical University Hospital

Locations

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Taipei Medical University Hospital

Taipei, Taiwan, Taiwan

Site Status

Countries

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Taiwan

References

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Brennecke J, Stark A, Russell RB, Cohen SM. Principles of microRNA-target recognition. PLoS Biol. 2005 Mar;3(3):e85. doi: 10.1371/journal.pbio.0030085.

Reference Type BACKGROUND
PMID: 15723116 (View on PubMed)

Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004 Jan 23;116(2):281-97. doi: 10.1016/s0092-8674(04)00045-5.

Reference Type BACKGROUND
PMID: 14744438 (View on PubMed)

Hilton C, Neville MJ, Karpe F. MicroRNAs in adipose tissue: their role in adipogenesis and obesity. Int J Obes (Lond). 2013 Mar;37(3):325-32. doi: 10.1038/ijo.2012.59. Epub 2012 Apr 24.

Reference Type BACKGROUND
PMID: 22531086 (View on PubMed)

Krotkiewski M, Bjorntorp P, Sjostrom L, Smith U. Impact of obesity on metabolism in men and women. Importance of regional adipose tissue distribution. J Clin Invest. 1983 Sep;72(3):1150-62. doi: 10.1172/JCI111040.

Reference Type BACKGROUND
PMID: 6350364 (View on PubMed)

Jiang L, Huang Q, Zhang S, Zhang Q, Chang J, Qiu X, Wang E. Hsa-miR-125a-3p and hsa-miR-125a-5p are downregulated in non-small cell lung cancer and have inverse effects on invasion and migration of lung cancer cells. BMC Cancer. 2010 Jun 22;10:318. doi: 10.1186/1471-2407-10-318.

Reference Type RESULT
PMID: 20569443 (View on PubMed)

Herrera BM, Lockstone HE, Taylor JM, Wills QF, Kaisaki PJ, Barrett A, Camps C, Fernandez C, Ragoussis J, Gauguier D, McCarthy MI, Lindgren CM. MicroRNA-125a is over-expressed in insulin target tissues in a spontaneous rat model of Type 2 Diabetes. BMC Med Genomics. 2009 Aug 18;2:54. doi: 10.1186/1755-8794-2-54.

Reference Type RESULT
PMID: 19689793 (View on PubMed)

John B, Enright AJ, Aravin A, Tuschl T, Sander C, Marks DS. Human MicroRNA targets. PLoS Biol. 2004 Nov;2(11):e363. doi: 10.1371/journal.pbio.0020363. Epub 2004 Oct 5.

Reference Type RESULT
PMID: 15502875 (View on PubMed)

Kanehisa M, Goto S. KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000 Jan 1;28(1):27-30. doi: 10.1093/nar/28.1.27.

Reference Type RESULT
PMID: 10592173 (View on PubMed)

Gustafson B. Adipose tissue, inflammation and atherosclerosis. J Atheroscler Thromb. 2010 Apr 30;17(4):332-41. doi: 10.5551/jat.3939. Epub 2010 Feb 3.

Reference Type RESULT
PMID: 20124732 (View on PubMed)

O'Connell J, Lynch L, Cawood TJ, Kwasnik A, Nolan N, Geoghegan J, McCormick A, O'Farrelly C, O'Shea D. The relationship of omental and subcutaneous adipocyte size to metabolic disease in severe obesity. PLoS One. 2010 Apr 1;5(4):e9997. doi: 10.1371/journal.pone.0009997.

Reference Type RESULT
PMID: 20376319 (View on PubMed)

Wellen KE, Hotamisligil GS. Inflammation, stress, and diabetes. J Clin Invest. 2005 May;115(5):1111-9. doi: 10.1172/JCI25102.

Reference Type RESULT
PMID: 15864338 (View on PubMed)

Niswender KD, Morrison CD, Clegg DJ, Olson R, Baskin DG, Myers MG Jr, Seeley RJ, Schwartz MW. Insulin activation of phosphatidylinositol 3-kinase in the hypothalamic arcuate nucleus: a key mediator of insulin-induced anorexia. Diabetes. 2003 Feb;52(2):227-31. doi: 10.2337/diabetes.52.2.227.

Reference Type RESULT
PMID: 12540590 (View on PubMed)

Other Identifiers

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CRC-06-09-10

Identifier Type: -

Identifier Source: org_study_id