The Use of the Hormone Kisspeptin in 'in Vitro Fertilisation' (IVF) Treatment

NCT ID: NCT01667406

Last Updated: 2021-06-08

Study Results

Results available

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE2

Total Enrollment

175 participants

Study Classification

INTERVENTIONAL

Study Start Date

2012-06-01

Study Completion Date

2016-10-11

Brief Summary

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We want to find out if kisspeptin is successful in stimulating oocyte maturation when it is used as a 'trigger' injection during IVF therapy for infertility.

Detailed Description

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Participants in the study will have a standard Gonadotrophin Releasing Hormone (GnRH) antagonist IVF cycle, but instead of having a trigger injection of hCG (human chorionic gonadotrophin) they will be randomised to receive one of 4 doses of kisspeptin injection.

Oocytes will be retrieved and graded by an embryologist to see whether or not they have matured.

The reproductive hormones Luteinising hormone, follicle stimulating hormone, oestradiol and progesterone will also be measured

Conditions

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Infertility

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Different dose for each volunteer
Primary Study Purpose

TREATMENT

Blinding Strategy

DOUBLE

Participants Caregivers
participants and IVF physicians were blinded.

Study Groups

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Kisspeptin-54, 1.6 single

Participant undergoing in vitro fertilisation (IVF) treatment will receive a single trigger injection of Kisspeptin, dose of 1.6 nmol/kg

Group Type EXPERIMENTAL

Kisspeptin 1.6nmol/kg

Intervention Type DRUG

single kisspeptin dose 1.6 nmol/kg subcutaneously

Kisspeptin-54, 3.2 single

Participant undergoing in vitro fertilisation (IVF) treatment will receive a single trigger injection of Kisspeptin, dose of 3.2 nmol/kg

Group Type EXPERIMENTAL

Kisspeptin 3.2nmol/kg

Intervention Type DRUG

single kisspeptin dose 3.2 nmol/kg subcutaneously

Kisspeptin-54, 6.4 single

Participant undergoing in vitro fertilisation (IVF) treatment will receive a single trigger injection of Kisspeptin, dose of 6.4 nmol/kg

Group Type EXPERIMENTAL

Kisspeptin 6.4nmol/kg

Intervention Type DRUG

single kisspeptin dose 6.4 nmol/kg subcutaneously

Kisspeptin-54, 12.8 single

Participant undergoing in vitro fertilisation (IVF) treatment will receive a single trigger injection of Kisspeptin, dose of 12.8 nmol/kg

Group Type EXPERIMENTAL

Kisspeptin 12.8nmol/kg

Intervention Type DRUG

single kisspeptin dose 12.8 nmol/kg subcutaneously

Kisspeptin-54 OHSS, 3.2 single

Participant undergoing in vitro fertilisation (IVF) treatment and who is at high risk of ovarian hyper stimulation syndrome (OHSS) will receive a single trigger injection of Kisspeptin, dose of 3.2 nmol/kg

Group Type EXPERIMENTAL

Kisspeptin 3.2nmol/kg

Intervention Type DRUG

single kisspeptin dose 3.2 nmol/kg subcutaneously

Kisspeptin-54 OHSS, 6.4 single

Participant undergoing in vitro fertilisation (IVF) treatment and who is at high risk of ovarian hyper stimulation syndrome (OHSS) will receive a single trigger injection of Kisspeptin, dose of 6.4 nmol/kg

Group Type EXPERIMENTAL

Kisspeptin 6.4nmol/kg

Intervention Type DRUG

single kisspeptin dose 6.4 nmol/kg subcutaneously

Kisspeptin-54 OHSS, 9.6 single

Participant undergoing in vitro fertilisation (IVF) treatment and who is at high risk of ovarian hyper stimulation syndrome (OHSS) will receive a single trigger injection of Kisspeptin, dose of 9.6 nmol/kg

Group Type EXPERIMENTAL

Kisspeptin 9.6nmol/kg

Intervention Type DRUG

single kisspeptin dose 9.6 nmol/kg subcutaneously

Kisspeptin-54 OHSS, 12.8 single

Participant undergoing in vitro fertilisation (IVF) treatment and who is at high risk of ovarian hyper stimulation syndrome (OHSS) will receive a single trigger injection of Kisspeptin, dose of 12.8 nmol/kg

Group Type EXPERIMENTAL

Kisspeptin 12.8nmol/kg

Intervention Type DRUG

single kisspeptin dose 12.8 nmol/kg subcutaneously

Kisspeptin-54 OHSS, 9.6 + 9.6

Participant undergoing in vitro fertilisation (IVF) treatment and who is at high risk of ovarian hyper stimulation syndrome (OHSS) will receive a trigger injection of Kisspeptin, dose of 9.6 nmol/kg and a further injection of Kisspeptin, dose of 9.6 nmol/kg 10 hours later

Group Type EXPERIMENTAL

Kisspeptin 9.6 nmol/kg double

Intervention Type DRUG

kisspeptin dose 9.6 nmol/kg given twice 10hrs apart subcutaneously

Kisspeptin-54 OHSS, 9.6 + saline

Participant undergoing in vitro fertilisation (IVF) treatment and who is at high risk of ovarian hyper stimulation syndrome (OHSS) will receive a trigger injection of Kisspeptin, dose of 9.6 nmol/kg and a further injection of saline10 hours later

Group Type EXPERIMENTAL

Kisspeptin 9.6 nmol/kg + saline

Intervention Type DRUG

kisspeptin dose 9.6 nmol/kg subcutaneously and saline subcutaneously, 10hrs apart

Interventions

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Kisspeptin 1.6nmol/kg

single kisspeptin dose 1.6 nmol/kg subcutaneously

Intervention Type DRUG

Kisspeptin 3.2nmol/kg

single kisspeptin dose 3.2 nmol/kg subcutaneously

Intervention Type DRUG

Kisspeptin 6.4nmol/kg

single kisspeptin dose 6.4 nmol/kg subcutaneously

Intervention Type DRUG

Kisspeptin 9.6nmol/kg

single kisspeptin dose 9.6 nmol/kg subcutaneously

Intervention Type DRUG

Kisspeptin 12.8nmol/kg

single kisspeptin dose 12.8 nmol/kg subcutaneously

Intervention Type DRUG

Kisspeptin 9.6 nmol/kg double

kisspeptin dose 9.6 nmol/kg given twice 10hrs apart subcutaneously

Intervention Type DRUG

Kisspeptin 9.6 nmol/kg + saline

kisspeptin dose 9.6 nmol/kg subcutaneously and saline subcutaneously, 10hrs apart

Intervention Type DRUG

Other Intervention Names

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kisspeptin-54 kisspeptin-54 kisspeptin-54 kisspeptin-54 kisspeptin-54 kisspeptin-54 kisspeptin-54

Eligibility Criteria

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Inclusion Criteria

* Aged 18 - 34 years
* Body mass index between 18 and 29 kg/m2
* Stable body weight for at least 3 months
* Normal early menstrual cycle follicular phase serum FSH concentration
* Serum anti-Mullerian hormone (AMH) \> 40pmol/L
* No more than one previous IVF treatment cycle
* Both ovaries intact

Exclusion Criteria

History of any medical, psychological or other condition, or use of any medications, including over-the-counter products, which, in the opinion of the investigators, would either interfere with the study or potentially cause harm to the volunteer

* Without access at home to a telephone, or other factor likely to interfere with ability to participate reliably in the study
* Treatment with an investigational drug within the preceding 2 months
* Donated blood during the preceding 3 months or intention to do so before the end of the study
* Previous poor response to IVF treatment
Minimum Eligible Age

18 Years

Maximum Eligible Age

34 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Imperial College London

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Waljit S Dhillo, PhD

Role: PRINCIPAL_INVESTIGATOR

Imperial College London

Locations

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Hammersmith Hospital

London, , United Kingdom

Site Status

Countries

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United Kingdom

References

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Jayasena CN, Abbara A, Comninos AN, Nijher GM, Christopoulos G, Narayanaswamy S, Izzi-Engbeaya C, Sridharan M, Mason AJ, Warwick J, Ashby D, Ghatei MA, Bloom SR, Carby A, Trew GH, Dhillo WS. Kisspeptin-54 triggers egg maturation in women undergoing in vitro fertilization. J Clin Invest. 2014 Aug;124(8):3667-77. doi: 10.1172/JCI75730. Epub 2014 Jul 18.

Reference Type RESULT
PMID: 25036713 (View on PubMed)

Abbara A, Clarke S, Islam R, Prague JK, Comninos AN, Narayanaswamy S, Papadopoulou D, Roberts R, Izzi-Engbeaya C, Ratnasabapathy R, Nesbitt A, Vimalesvaran S, Salim R, Lavery SA, Bloom SR, Huson L, Trew GH, Dhillo WS. A second dose of kisspeptin-54 improves oocyte maturation in women at high risk of ovarian hyperstimulation syndrome: a Phase 2 randomized controlled trial. Hum Reprod. 2017 Sep 1;32(9):1915-1924. doi: 10.1093/humrep/dex253.

Reference Type RESULT
PMID: 28854728 (View on PubMed)

Abbara A, Jayasena CN, Christopoulos G, Narayanaswamy S, Izzi-Engbeaya C, Nijher GM, Comninos AN, Peters D, Buckley A, Ratnasabapathy R, Prague JK, Salim R, Lavery SA, Bloom SR, Szigeti M, Ashby DA, Trew GH, Dhillo WS. Efficacy of Kisspeptin-54 to Trigger Oocyte Maturation in Women at High Risk of Ovarian Hyperstimulation Syndrome (OHSS) During In Vitro Fertilization (IVF) Therapy. J Clin Endocrinol Metab. 2015 Sep;100(9):3322-31. doi: 10.1210/jc.2015-2332. Epub 2015 Jul 20.

Reference Type RESULT
PMID: 26192876 (View on PubMed)

Abbara A, Islam R, Clarke SA, Jeffers L, Christopoulos G, Comninos AN, Salim R, Lavery SA, Vuong TNL, Humaidan P, Kelsey TW, Trew GH, Dhillo WS. Clinical parameters of ovarian hyperstimulation syndrome following different hormonal triggers of oocyte maturation in IVF treatment. Clin Endocrinol (Oxf). 2018 Jun;88(6):920-927. doi: 10.1111/cen.13569. Epub 2018 Mar 6.

Reference Type DERIVED
PMID: 29446481 (View on PubMed)

Owens LA, Abbara A, Lerner A, O'floinn S, Christopoulos G, Khanjani S, Islam R, Hardy K, Hanyaloglu AC, Lavery SA, Dhillo WS, Franks S. The direct and indirect effects of kisspeptin-54 on granulosa lutein cell function. Hum Reprod. 2018 Feb 1;33(2):292-302. doi: 10.1093/humrep/dex357.

Reference Type DERIVED
PMID: 29206944 (View on PubMed)

Provided Documents

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Document Type: Study Protocol

View Document

Document Type: Statistical Analysis Plan

View Document

Other Identifiers

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2012-000154-61

Identifier Type: EUDRACT_NUMBER

Identifier Source: secondary_id

13HH0199

Identifier Type: -

Identifier Source: org_study_id

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