Effect of Intravitreal Bone Marrow Stem Cells on Ischemic Retinopathy

NCT ID: NCT01518842

Last Updated: 2018-10-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

30 participants

Study Classification

INTERVENTIONAL

Study Start Date

2011-09-30

Study Completion Date

2020-01-31

Brief Summary

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This study aims to evaluate the behavior of the intravitreal use of bone marrow derived stem cells in patients with ischemic retinopathy.

Detailed Description

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Evaluate the effects on visual acuity, electroretinography, fluorescein angiography and optical coherence tomography in 30 patients with ischemic retinopathy, including diabetic retinopathy with severe loss of retinal capillaries undergoing intravitreal injection of bone marrow derived stem cells.

Conditions

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Ischemia

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

intravitreal injection of autologous bone marrow stem cells
Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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test group intravitreal stem cell

Open-label study of Ischemic Retinopathy patients with best-corrected visual acuity (BCVA) worse than 20/200.

Intervention: Biological: intravitreal injection of autologous bone marrow stem cells

Group Type EXPERIMENTAL

Intravitreal Bone Marrow Stem Cells

Intervention Type BIOLOGICAL

One intravitreal injection of a 0.1-ml cell suspension containing around 10x106 bone marrow mononuclear stem cells(BMMSC). All treatments were performed by a single retinal specialist using topical proparacaine drops under sterile conditions (eyelid speculum and povidone-iodine). Autologous BMMSC were injected into the vitreous cavity using a 27 gauge needle inserted through the inferotemporal pars plana 3.0 - 3.5 mm posterior to the limbus. After the injection, central retinal artery perfusion was confirmed with indirect ophthalmoscopy. Patients were instructed to instill one drop of 0.3% ciprofloxacin into the injected eye four times daily for 1 week after the procedure.

Interventions

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Intravitreal Bone Marrow Stem Cells

One intravitreal injection of a 0.1-ml cell suspension containing around 10x106 bone marrow mononuclear stem cells(BMMSC). All treatments were performed by a single retinal specialist using topical proparacaine drops under sterile conditions (eyelid speculum and povidone-iodine). Autologous BMMSC were injected into the vitreous cavity using a 27 gauge needle inserted through the inferotemporal pars plana 3.0 - 3.5 mm posterior to the limbus. After the injection, central retinal artery perfusion was confirmed with indirect ophthalmoscopy. Patients were instructed to instill one drop of 0.3% ciprofloxacin into the injected eye four times daily for 1 week after the procedure.

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* diagnosis of Ischemic Retinopathy
* Enlargement of foveal avascular zone-FAZ (ischaemia or capillary drop out of \>30% on Fluorescein retinography
* logarithm of minimum angle of resolution (logMAR) BCVA of 1.0 (Snellen equivalent, 20/200) or worse
* Able (in the Investigator's opinion) and willing to comply with all study requirements

Exclusion Criteria

* previous ocular surgery other than cataract
* presence of cataract or other media opacity that would influence ocular fundus documentation and adequate ERG and visual field evaluation
* other ophthalmic disease like glaucoma and uveitis
* previous history of blood disorders like leukemia
* known allergy to fluorescein or indocyanine green
Minimum Eligible Age

18 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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University of Sao Paulo

OTHER

Sponsor Role lead

Responsible Party

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Rubens Camargo Siqueira

MD,PhD - Principal Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Rubens C Siqueira, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Rubens Siqueira Research Center

Locations

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Rubens Siqueira Research Center

São José do Rio Preto, São Paulo, Brazil

Site Status

Countries

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Brazil

References

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Siqueira RC, Messias A, Gurgel VP, Simoes BP, Scott IU, Jorge R. Improvement of ischaemic macular oedema after intravitreal injection of autologous bone marrow-derived haematopoietic stem cells. Acta Ophthalmol. 2015 Mar;93(2):e174-6. doi: 10.1111/aos.12473. Epub 2014 Jun 22. No abstract available.

Reference Type BACKGROUND
PMID: 24954079 (View on PubMed)

Other Identifiers

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Siqueira Research Center

Identifier Type: -

Identifier Source: org_study_id

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