Safety and Immunogenicity Study of HIV-MAG Vaccine +/- IL-12 and Ad35-GRIN/ENV in HIV-uninfected Volunteers

NCT ID: NCT01496989

Last Updated: 2013-09-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

75 participants

Study Classification

INTERVENTIONAL

Study Start Date

2011-12-31

Brief Summary

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The purpose of this study is to evaluate the safety, tolerability and immunogenicity of multiantigen HIV (HIV-MAG) plasmid DNA (pDNA) vaccine co-administered with recombinant human IL-12 pDNA (GENEVAX® IL-12) followed or preceded by recombinant Ad35-GRIN/ENV HIV vaccine in low-risk for HIV-uninfected healthy adults.

Detailed Description

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The study is a randomized, double-blind placebo-controlled trial assessing the safety and tolerability of HIV-MAG with or without co-administered GENEVAX® IL-12 given intramuscularly by in vivo electroporation (IM/EP) using the Ichor Medical Systems TriGrid Delivery System (TDS-IM) followed by Ad35-GRIN/ENV in each of four different regimens. An additional group will evaluate the safety and tolerability of Ad35-GRIN/ENV followed by HIV-MAG with co-administered GENEVAX® IL-12 given intramuscularly by in vivo electroporation (IM/EP) using the Ichor Medical Systems TriGrid Delivery System (TDS-IM).

Volunteers will be screened up to 42 days before the 1st vaccination and will be followed for 12 months after the first vaccine administration. It is anticipated that it will take approximately 3 months to enrol the study. Approximately 75 volunteers (60 vaccine/15 placebo recipients) will be included in the study.

Conditions

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HIV Infections

Keywords

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HIV AIDS HIV vaccine HIV prevention

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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Group 5: Ad35-GRIN/ENV followed by HIV-MAG+GENEVAX® IL-12

Ad35-GRIN/ENV (IM) at Month 0 followed by HIV-MAG + GENEVAX® IL-12 (IM/EP) at Month 4. (Vaccine:Placebo=12/3)

Group Type EXPERIMENTAL

HIV-MAG (3,000mcg)

Intervention Type BIOLOGICAL

Delivered intramuscularly by in vivo electroporation

GENEVAX® IL-12 (1000mcg)

Intervention Type BIOLOGICAL

Co-administered with HIV-MAG, delivered intramuscular by in vivo electroporation

Ad35-GRIN/ENV

Intervention Type BIOLOGICAL

(2x10\^10vp) Delivered intramuscularly by standard needle injection

Group 1: HIV-MAG followed by Ad35-GRIN/ENV

HIV-MAG (IM/EP) at Months 0,1,2 followed by Ad35-GRIN/ENV (IM) at Month 6. (Vaccine:Placebo = 12/3)

Group Type EXPERIMENTAL

HIV-MAG (3,000mcg)

Intervention Type BIOLOGICAL

Delivered intramuscularly by in vivo electroporation

Ad35-GRIN/ENV

Intervention Type BIOLOGICAL

(2x10\^10vp) Delivered intramuscularly by standard needle injection

Group 2: HIV-MAG+GENEVAX® IL-12 followed by Ad35-GRIN/ENV

HIV-MAG + GENEVAX® IL-12 (IM/EP) at Months 0,1,2 followed by Ad35-GRIN/ENV (IM) at Month 6. (Vaccine:Placebo=12/3)

Group Type EXPERIMENTAL

HIV-MAG (3,000mcg)

Intervention Type BIOLOGICAL

Delivered intramuscularly by in vivo electroporation

GENEVAX® IL-12 (100mcg)

Intervention Type BIOLOGICAL

Co-administered with HIV-MAG, delivered intramuscularly by in vivo electroporation

Ad35-GRIN/ENV

Intervention Type BIOLOGICAL

(2x10\^10vp) Delivered intramuscularly by standard needle injection

Group 3: HIV-MAG+GENEVAX® IL-12 followed by Ad35-GRIN/ENV

HIV-MAG + GENEVAX® IL-12 (IM/EP) at Months 0,1,2 followed by Ad35-GRIN/ENV (IM) at Month 6. (Vaccine:Placebo= 12/3)

Group Type EXPERIMENTAL

HIV-MAG (3,000mcg)

Intervention Type BIOLOGICAL

Delivered intramuscularly by in vivo electroporation

GENEVAX® IL-12 (1000mcg)

Intervention Type BIOLOGICAL

Co-administered with HIV-MAG, delivered intramuscular by in vivo electroporation

Ad35-GRIN/ENV

Intervention Type BIOLOGICAL

(2x10\^10vp) Delivered intramuscularly by standard needle injection

Group 4: HIV-MAG+GENEVAX® IL-12 followed by Ad35-GRIN/ENV

HIV-MAG + GENEVAX® IL-12 (IM/EP) at Month 0 followed by Ad35-GRIN/ENV (IM) at Month 4. (Vaccine:Placebo=12/3)

Group Type EXPERIMENTAL

HIV-MAG (3,000mcg)

Intervention Type BIOLOGICAL

Delivered intramuscularly by in vivo electroporation

GENEVAX® IL-12 (1000mcg)

Intervention Type BIOLOGICAL

Co-administered with HIV-MAG, delivered intramuscular by in vivo electroporation

Ad35-GRIN/ENV

Intervention Type BIOLOGICAL

(2x10\^10vp) Delivered intramuscularly by standard needle injection

Interventions

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HIV-MAG (3,000mcg)

Delivered intramuscularly by in vivo electroporation

Intervention Type BIOLOGICAL

GENEVAX® IL-12 (100mcg)

Co-administered with HIV-MAG, delivered intramuscularly by in vivo electroporation

Intervention Type BIOLOGICAL

GENEVAX® IL-12 (1000mcg)

Co-administered with HIV-MAG, delivered intramuscular by in vivo electroporation

Intervention Type BIOLOGICAL

Ad35-GRIN/ENV

(2x10\^10vp) Delivered intramuscularly by standard needle injection

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* healthy male or female adults,
* 18 to 50 years of age (21 to 50 years of age for volunteers in Rwanda),
* who do not report high-risk behaviour for HIV infection,
* who are available for the duration of the trial,
* who are willing to undergo HIV testing,
* use an effective method of contraception, and
* who, in the opinion of the principal investigator or designee, understand the study and who provide written informed consent.

Exclusion Criteria

* confirmed HIV infection,
* pregnancy and lactation,
* significant acute or chronic disease,
* clinically significant laboratory abnormalities,
* recent vaccination or receipt of a blood product,
* previous receipt of an HIV vaccine, and
* previous severe local or systemic reactions to vaccination or history of severe allergic reactions.
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Auro Vaccines LLC

INDUSTRY

Sponsor Role collaborator

Ichor Medical Systems Incorporated

INDUSTRY

Sponsor Role collaborator

International AIDS Vaccine Initiative

NETWORK

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Kenya AIDS Vaccine Initiative, Kangemi

Nairobi, , Kenya

Site Status

Projet San Francisco

Kigali, , Rwanda

Site Status

Uganda Virus Research Institute-IAVI

Entebbe, , Uganda

Site Status

Countries

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Kenya Rwanda Uganda

References

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Mpendo J, Mutua G, Nanvubya A, Anzala O, Nyombayire J, Karita E, Dally L, Hannaman D, Price M, Fast PE, Priddy F, Gelderblom HC, Hills NK. Acceptability and tolerability of repeated intramuscular electroporation of Multi-antigenic HIV (HIVMAG) DNA vaccine among healthy African participants in a phase 1 randomized controlled trial. PLoS One. 2020 May 29;15(5):e0233151. doi: 10.1371/journal.pone.0233151. eCollection 2020.

Reference Type DERIVED
PMID: 32469893 (View on PubMed)

Mpendo J, Mutua G, Nyombayire J, Ingabire R, Nanvubya A, Anzala O, Karita E, Hayes P, Kopycinski J, Dally L, Hannaman D, Egan MA, Eldridge JH, Syvertsen K, Lehrman J, Rasmussen B, Gilmour J, Cox JH, Fast PE, Schmidt C. A Phase I Double Blind, Placebo-Controlled, Randomized Study of the Safety and Immunogenicity of Electroporated HIV DNA with or without Interleukin 12 in Prime-Boost Combinations with an Ad35 HIV Vaccine in Healthy HIV-Seronegative African Adults. PLoS One. 2015 Aug 7;10(8):e0134287. doi: 10.1371/journal.pone.0134287. eCollection 2015.

Reference Type DERIVED
PMID: 26252526 (View on PubMed)

Related Links

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http://www.iavi.org

International AIDS Vaccine Initiative

http://www.ichorms.com/

Ichor Medical Systems, Inc.

Other Identifiers

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IAVI B004

Identifier Type: -

Identifier Source: org_study_id