Safety and Effectiveness of PENNVAX-B Vaccine Alone, With Il-12, or IL-15 in Healthy Adults

NCT ID: NCT00528489

Last Updated: 2021-10-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

120 participants

Study Classification

INTERVENTIONAL

Study Start Date

2007-10-31

Study Completion Date

2010-01-31

Brief Summary

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The purpose of this study is to determine the safety, tolerability, and immune response to the DNA HIV vaccine, PENNVAX-B alone, in combination with IL-12, or with 2 different doses of IL-15.

Detailed Description

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A vaccine that is effective against multiple strains of HIV remains the best option for preventing the spread of HIV. Plasmid DNA vaccines are inexpensive and easy to construct. When DNA vaccines are administered in combination with cytokines, such as IL-12 or IL-15, the immune response to the vaccine is increased. The purpose of this study is to determine the safety and tolerability of the DNA plasmid vaccine, PENNVAX-B when administered alone, or with IL-12 or IL-15 DNA adjuvants. The most effective and safe dose of IL-15 will also be determined during this study.

This study will last approximately 12 months. Participants will be randomly assigned to one of four groups. Group 1 will receive an injection of PENNVAX-B combined with 0.8 mg IL-15 in each deltoid or placebo. Group 2 will receive an injection of PENNVAX-B alone or placebo. Group 3 will receive an injection of PENNVAX-B combined with IL-12 in each deltoid or placebo. Group 4 will receive PENNVAX-B combined with 2 mg IL-15 or placebo. Injections will occur at study entry, Months 1, 3, and 6. Additional study visits will occur on Days 14, 42, 98, 182, 273, and 364. At these visits a brief physical, blood collection, interview, and risk reduction counseling will occur. At some visits HIV testing, urine collection, and pregnancy tests will also occur.

As of 05/30/08, participants will be enrolled into Groups 3 and 4 at a limited pace to allow for additional safety reviews for the first few participants in these groups. Additionally, safety data will be reviewed for Groups 1 and 2 to determine whether the remainder of participants in Groups 2, 3, and 4 will be enrolled.

Conditions

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HIV Infections

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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1

PENNVAX-B with 0.8 mg IL-15 administered in both deltoids at Months 0, 1, 3, and 6

Group Type EXPERIMENTAL

PennVax B

Intervention Type BIOLOGICAL

DNA vaccine containing the HIV genes Gag, Pol, and Env

IL-15

Intervention Type BIOLOGICAL

Cytokine injection

2

PENNVAX-B administered alone in both deltoids at Months 0, 1, 3, and 6

Group Type EXPERIMENTAL

PennVax B

Intervention Type BIOLOGICAL

DNA vaccine containing the HIV genes Gag, Pol, and Env

3

PENNVAX-B administered alone in both deltoids at Months 0, 1, 3, and 6

Group Type EXPERIMENTAL

PennVax B

Intervention Type BIOLOGICAL

DNA vaccine containing the HIV genes Gag, Pol, and Env

IL-12

Intervention Type BIOLOGICAL

Cytokine injection

4

PENNVAX-B with 2 mg IL-15 injected into both deltoids at Months 0, 1, 3, and 6

Group Type EXPERIMENTAL

PennVax B

Intervention Type BIOLOGICAL

DNA vaccine containing the HIV genes Gag, Pol, and Env

IL-15

Intervention Type BIOLOGICAL

Cytokine injection

Interventions

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PennVax B

DNA vaccine containing the HIV genes Gag, Pol, and Env

Intervention Type BIOLOGICAL

IL-12

Cytokine injection

Intervention Type BIOLOGICAL

IL-15

Cytokine injection

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* HIV-1 and -2 uninfected
* Willing to receive HIV test results
* Good general health
* Certain laboratory values within normal range or with site physician approval
* Negative for Hepatitis B surface antigen
* Negative Hepatitis C test
* Willing to use acceptable methods of contraception

Exclusion Criteria

* HIV vaccines or placebos in prior HIV trial. Participants who can provide documentation that they received a placebo in a prior HIV trial may be eligible.
* Immunosuppressive medications within 168 days prior to first study vaccination. Participants using corticosteroid nasal spray for allergies or topical corticosteroids for mild, uncomplicated dermatitis are not excluded.
* Blood products within 120 days prior to first study vaccination
* Receipt of immunoglobulin within 60 days prior to first vaccination
* Live attenuated vaccines within 30 days prior to first study vaccination
* Any vaccine that is not a live attenuated vaccine within 14 days prior to first study vaccination
* Investigational research agents within 60 days prior to first study vaccination
* Current anti-tuberculosis (TB) preventive therapy or treatment clinically significant medical condition, abnormal physical exam findings, abnormal laboratory results, or past medical history that may affect current health. More information about this criterion can be found in the protocol.
* Any medical, psychiatric, social, occupational, or other condition or responsibility that in the opinion of the investigator would interfere with the study. More information about this criterion can be found in the protocol.
* Allergy to amide-type local anesthetics
* Serious adverse reactions to vaccines
* Autoimmune disease or immunodeficiency
* Active syphilis infection. Participants who have been fully treated for syphilis over 6 months prior to study entry are not excluded.
* Asthma other than mild, well-controlled asthma. More information on this criterion can be found in the protocol.
* Type 1 or type 2 diabetes mellitus. Participants with histories of isolated gestational diabetes are not excluded.
* Thyroidectomy or thyroid disease requiring medication during the 12 months prior to study entry
* Accumulation of fluid in the blood vessels (angioedema) within 3 years prior to study entry if episodes are considered serious or have required medication in the 2 years prior to study entry
* Uncontrolled hypertension
* Body Mass Index of 40 or greater OR of 35 or greater if certain other criteria apply. More information about these criteria can be found in the protocol
* Bleeding disorder
* Cancer. Participants with surgically removed cancer that is unlikely to recur are not excluded.
* Seizure disorder
* Absence of the spleen
* Pregnancy or breastfeeding
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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HIV Vaccine Trials Network

NETWORK

Sponsor Role collaborator

National Institute of Allergy and Infectious Diseases (NIAID)

NIH

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Scott Parker, MD

Role: STUDY_CHAIR

HVTN, FHCRC

Locations

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Alabama Vaccine CRS

Birmingham, Alabama, United States

Site Status

San Francisco Vaccine and Prevention CRS

San Francisco, California, United States

Site Status

Brigham and Women's Hosp. CRS

Boston, Massachusetts, United States

Site Status

NY Blood Ctr./Union Square CRS

New York, New York, United States

Site Status

HIV Prevention & Treatment CRS

New York, New York, United States

Site Status

NY Blood Ctr./Bronx CRS

The Bronx, New York, United States

Site Status

3535 Market Street CRS

Philadelphia, Pennsylvania, United States

Site Status

Vanderbilt Vaccine CRS

Nashville, Tennessee, United States

Site Status

Countries

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United States

References

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Johnston MI, Fauci AS. An HIV vaccine--evolving concepts. N Engl J Med. 2007 May 17;356(20):2073-81. doi: 10.1056/NEJMra066267. No abstract available.

Reference Type BACKGROUND
PMID: 17507706 (View on PubMed)

Ogawa H, Ueno M, Uchibori H, Matsumoto I, Seno N. Direct carbohydrate analysis of glycoproteins electroblotted onto polyvinylidene difluoride membrane from sodium dodecyl sulfate-polyacrylamide gel. Anal Biochem. 1990 Nov 1;190(2):165-9. doi: 10.1016/0003-2697(90)90175-9.

Reference Type BACKGROUND
PMID: 1705391 (View on PubMed)

McBurney SP, Ross TM. Developing broadly reactive HIV-1/AIDS vaccines: a review of polyvalent and centralized HIV-1 vaccines. Curr Pharm Des. 2007;13(19):1957-64. doi: 10.2174/138161207781039841.

Reference Type BACKGROUND
PMID: 17627529 (View on PubMed)

Jin X, Morgan C, Yu X, DeRosa S, Tomaras GD, Montefiori DC, Kublin J, Corey L, Keefer MC; NIAID HIV Vaccine Trials Network. Multiple factors affect immunogenicity of DNA plasmid HIV vaccines in human clinical trials. Vaccine. 2015 May 11;33(20):2347-53. doi: 10.1016/j.vaccine.2015.03.036. Epub 2015 Mar 25.

Reference Type DERIVED
PMID: 25820067 (View on PubMed)

Related Links

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http://www.hvtn.org

Click here to learn more about the HIV Vaccine Trials Network

Other Identifiers

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10490

Identifier Type: REGISTRY

Identifier Source: secondary_id

HVTN 070

Identifier Type: -

Identifier Source: org_study_id