AC220 for Children With Relapsed/Refractory ALL or AML

NCT ID: NCT01411267

Last Updated: 2022-04-04

Study Results

Results available

Outcome measurements, participant flow, baseline characteristics, and adverse events have been published for this study.

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

24 participants

Study Classification

INTERVENTIONAL

Study Start Date

2011-09-01

Study Completion Date

2013-09-12

Brief Summary

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This is a phase I study of the investigational drug AC220 combined with cytarabine and etoposide in pediatric patients with relapsed acute lymphoblastic leukemia (ALL) and acute myelogenous leukemia (AML).

Detailed Description

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This is a study for pediatric patients with relapsed acute lymphoblastic leukemia (ALL) or acute myelogenous leukemia (AML). Some people diagnosed with leukemia have changes in a receptor located on the surface of white blood cells called FLT3. This is known as a FLT3 mutation. FLT3 plays an important role in the way cells grow and divide. In normal cells, the FLT3 receptor is switched off most of the time and only switches on when it gets a chemical signal from outside. But cells with the FLT3 mutation have the grow signal permanently switched on. This means leukemia cells with the FLT3 mutation are growing and dividing all the time. Doctors have found that people with leukemia cells that carry FLT3 mutations are less likely to go into remission with chemotherapy and have a higher risk of the leukemia coming back after treatment.

This is a study of an investigational drug called AC220. AC220 is considered investigational because it has not been approved in the United States by the Food and Drug Administration (FDA). AC220 is a drug which is able to "turn off" the FLT3 grow signal. AC220 will be given with cytarabine and etoposide to treat the relapsed leukemia. This is a phase I study, which means that the study is being done to find the highest dose of AC220 that can be given safely with the drugs cytarabine and etoposide to children and young adults.

Conditions

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Lymphoblastic Leukemia, Acute, Childhood Myelogenous Leukemia, Acute, Childhood

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SEQUENTIAL

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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ALL AC220 @ 25mg/m2/day (Dose Level 1)

The starting dose is Dose Level 1 at 25 mg/m2/day. Dose escalation will proceed from level 1 to 2 to 3, and so on, assuming the maximum tolerated dose is not exceeded. Patients will received etoposide and cytarabine on Days 1-5, and AC220 on Day 7 through 28. IT Methotrexate will be given to patients with ALL on Day 0, dose assigned by age.

Group Type EXPERIMENTAL

AC220

Intervention Type DRUG

Dose assigned at study entry. AC220 will be given orally once daily on days 7-28.

Cytarabine

Intervention Type DRUG

All patients receive 1000 mg/m2/day IV given every 12 hours on days 1 through 5. Additionally, AML patients and patients with ambiguous leukemia receive cytarabine intrathecally on day "1" of course 1 and 2. Dose defined by age:

* 20 mg for patients age \<1 yr
* 30 mg for patients age 1-1.99 years of age
* 50 mg for patients age 2-2.99 years of age
* 70 mg for patients \>3 years of age

Etoposide

Intervention Type DRUG

150 mg/m2/day IV on days 1 through 5.

Methotrexate

Intervention Type DRUG

IT methotrexate given intrathecally to patients with ALL on day "0" of course 1 and 2. Dose defined by age

* 6 mg for patients age \< 1yr
* 8 mg for patients age 1-1.99
* 10 mg for patients age 2-2.99
* 12 mg for patients 3-8.99 years of age
* 15 mg for patients \>9 years of age

AML AC220 @ 25mg/m2/day (Dose Level 1)

The starting dose is Dose Level 1 at 25 mg/m2/day. Dose escalation will proceed from level 1 to 2 to 3, and so on, assuming the maximum tolerated dose is not exceeded. Patients will received etoposide and cytarabine on Days 1-5, and AC220 on Day 7 through 28. IT cytarabine will be given to patients with AML on Day 0, dose assigned by age.

Group Type EXPERIMENTAL

AC220

Intervention Type DRUG

Dose assigned at study entry. AC220 will be given orally once daily on days 7-28.

Cytarabine

Intervention Type DRUG

All patients receive 1000 mg/m2/day IV given every 12 hours on days 1 through 5. Additionally, AML patients and patients with ambiguous leukemia receive cytarabine intrathecally on day "1" of course 1 and 2. Dose defined by age:

* 20 mg for patients age \<1 yr
* 30 mg for patients age 1-1.99 years of age
* 50 mg for patients age 2-2.99 years of age
* 70 mg for patients \>3 years of age

Etoposide

Intervention Type DRUG

150 mg/m2/day IV on days 1 through 5.

ALL AC220 @ 40mg/m2/day (Dose Level 2)

Dose escalation will proceed from level 1 to level 2 for AC220 at 40mg/m2/day, assuming the maximum tolerated dose is not exceeded. Patients will received etoposide and cytarabine on Days 1-5, and AC220 on Day 7 through 28. IT Methotrexate will be given to patients with ALL on Day 0, dose assigned by age.

Group Type EXPERIMENTAL

AC220

Intervention Type DRUG

Dose assigned at study entry. AC220 will be given orally once daily on days 7-28.

Cytarabine

Intervention Type DRUG

All patients receive 1000 mg/m2/day IV given every 12 hours on days 1 through 5. Additionally, AML patients and patients with ambiguous leukemia receive cytarabine intrathecally on day "1" of course 1 and 2. Dose defined by age:

* 20 mg for patients age \<1 yr
* 30 mg for patients age 1-1.99 years of age
* 50 mg for patients age 2-2.99 years of age
* 70 mg for patients \>3 years of age

Etoposide

Intervention Type DRUG

150 mg/m2/day IV on days 1 through 5.

Methotrexate

Intervention Type DRUG

IT methotrexate given intrathecally to patients with ALL on day "0" of course 1 and 2. Dose defined by age

* 6 mg for patients age \< 1yr
* 8 mg for patients age 1-1.99
* 10 mg for patients age 2-2.99
* 12 mg for patients 3-8.99 years of age
* 15 mg for patients \>9 years of age

ALL AC220 @ 60mg/m2/day (Dose Level 3)

Dose escalation will proceed from level 2 to 3 for AC220 at 60mg/m2/day, assuming the maximum tolerated dose is not exceeded. Patients will received etoposide and cytarabine on Days 1-5, and AC220 on Day 7 through 28. IT Methotrexate will be given to patients with ALL on Day 0, dose assigned by age. If toxicity at Dose Level 3 would allow further escalation, but demonstrated sufficient AC220 activity, no further dose escalation will be required.

Group Type EXPERIMENTAL

AC220

Intervention Type DRUG

Dose assigned at study entry. AC220 will be given orally once daily on days 7-28.

Cytarabine

Intervention Type DRUG

All patients receive 1000 mg/m2/day IV given every 12 hours on days 1 through 5. Additionally, AML patients and patients with ambiguous leukemia receive cytarabine intrathecally on day "1" of course 1 and 2. Dose defined by age:

* 20 mg for patients age \<1 yr
* 30 mg for patients age 1-1.99 years of age
* 50 mg for patients age 2-2.99 years of age
* 70 mg for patients \>3 years of age

Etoposide

Intervention Type DRUG

150 mg/m2/day IV on days 1 through 5.

Methotrexate

Intervention Type DRUG

IT methotrexate given intrathecally to patients with ALL on day "0" of course 1 and 2. Dose defined by age

* 6 mg for patients age \< 1yr
* 8 mg for patients age 1-1.99
* 10 mg for patients age 2-2.99
* 12 mg for patients 3-8.99 years of age
* 15 mg for patients \>9 years of age

ALL AC220 @ 90mg/m2/day (Dose Level 4)

If the study dose of 60 mg/m2/day at Dose Level 3 is well tolerated but does not show sufficient AC220 activity, the study may proceed to Dose Level 4 at 90 mg/m2/day. Patients will received etoposide and cytarabine on Days 1-5, and AC220 on Day 7 through 28. IT Methotrexate will be given to patients with ALL on Day 0, dose assigned by age. If toxicity at Dose Level 4 would allow further escalation, but demonstrated sufficient AC220 activity, no further dose escalation will be required.

Group Type EXPERIMENTAL

AC220

Intervention Type DRUG

Dose assigned at study entry. AC220 will be given orally once daily on days 7-28.

Cytarabine

Intervention Type DRUG

All patients receive 1000 mg/m2/day IV given every 12 hours on days 1 through 5. Additionally, AML patients and patients with ambiguous leukemia receive cytarabine intrathecally on day "1" of course 1 and 2. Dose defined by age:

* 20 mg for patients age \<1 yr
* 30 mg for patients age 1-1.99 years of age
* 50 mg for patients age 2-2.99 years of age
* 70 mg for patients \>3 years of age

Etoposide

Intervention Type DRUG

150 mg/m2/day IV on days 1 through 5.

Methotrexate

Intervention Type DRUG

IT methotrexate given intrathecally to patients with ALL on day "0" of course 1 and 2. Dose defined by age

* 6 mg for patients age \< 1yr
* 8 mg for patients age 1-1.99
* 10 mg for patients age 2-2.99
* 12 mg for patients 3-8.99 years of age
* 15 mg for patients \>9 years of age

ALL AC220 @ 130 mg/m2/day (Dose Level 5)

If the study dose of 90 mg/m2/day at Dose Level 4 is well tolerated but does not show sufficient AC220 activity, the study may proceed to Dose Level 5 at 130 mg/m2/day. Patients will received etoposide and cytarabine on Days 1-5, and AC220 on Day 7 through 28. IT Methotrexate will be given to patients with ALL on Day 0, dose assigned by age. Dose Level 5 is the highest dose for this study.

Group Type EXPERIMENTAL

AC220

Intervention Type DRUG

Dose assigned at study entry. AC220 will be given orally once daily on days 7-28.

Cytarabine

Intervention Type DRUG

All patients receive 1000 mg/m2/day IV given every 12 hours on days 1 through 5. Additionally, AML patients and patients with ambiguous leukemia receive cytarabine intrathecally on day "1" of course 1 and 2. Dose defined by age:

* 20 mg for patients age \<1 yr
* 30 mg for patients age 1-1.99 years of age
* 50 mg for patients age 2-2.99 years of age
* 70 mg for patients \>3 years of age

Etoposide

Intervention Type DRUG

150 mg/m2/day IV on days 1 through 5.

Methotrexate

Intervention Type DRUG

IT methotrexate given intrathecally to patients with ALL on day "0" of course 1 and 2. Dose defined by age

* 6 mg for patients age \< 1yr
* 8 mg for patients age 1-1.99
* 10 mg for patients age 2-2.99
* 12 mg for patients 3-8.99 years of age
* 15 mg for patients \>9 years of age

AML AC220 @ 40mg/m2/day (Dose Level 2)

Dose escalation will proceed from level 1 to level 2 for AC220 at 40mg/m2/day, assuming the maximum tolerated dose is not exceeded. Patients will received etoposide and cytarabine on Days 1-5, and AC220 on Day 7 through 28. IT cytarabine will be given to patients with AML on Day 0, dose assigned by age.

Group Type EXPERIMENTAL

AC220

Intervention Type DRUG

Dose assigned at study entry. AC220 will be given orally once daily on days 7-28.

Cytarabine

Intervention Type DRUG

All patients receive 1000 mg/m2/day IV given every 12 hours on days 1 through 5. Additionally, AML patients and patients with ambiguous leukemia receive cytarabine intrathecally on day "1" of course 1 and 2. Dose defined by age:

* 20 mg for patients age \<1 yr
* 30 mg for patients age 1-1.99 years of age
* 50 mg for patients age 2-2.99 years of age
* 70 mg for patients \>3 years of age

Etoposide

Intervention Type DRUG

150 mg/m2/day IV on days 1 through 5.

AML AC220 @ 60mg/m2/day (Dose Level 3)

Dose escalation will proceed from level 2 to 3 for AC220 at 60mg/m2/day, assuming the maximum tolerated dose is not exceeded. Patients will received etoposide and cytarabine on Days 1-5, and AC220 on Day 7 through 28. IT cytarabine will be given to patients with AML on Day 0, dose assigned by age. If toxicity at Dose Level 3 would allow further escalation, but demonstrated sufficient AC220 activity, no further dose escalation will be required.

Group Type EXPERIMENTAL

AC220

Intervention Type DRUG

Dose assigned at study entry. AC220 will be given orally once daily on days 7-28.

Cytarabine

Intervention Type DRUG

All patients receive 1000 mg/m2/day IV given every 12 hours on days 1 through 5. Additionally, AML patients and patients with ambiguous leukemia receive cytarabine intrathecally on day "1" of course 1 and 2. Dose defined by age:

* 20 mg for patients age \<1 yr
* 30 mg for patients age 1-1.99 years of age
* 50 mg for patients age 2-2.99 years of age
* 70 mg for patients \>3 years of age

Etoposide

Intervention Type DRUG

150 mg/m2/day IV on days 1 through 5.

AML AC220 @ 90mg/m2/day (Dose Level 4)

If the study dose of 60 mg/m2/day at Dose Level 3 is well tolerated but does not show sufficient AC220 activity, the study may proceed to Dose Level 4 at 90 mg/m2/day. Patients will received etoposide and cytarabine on Days 1-5, and AC220 on Day 7 through 28. IT cytarabine will be given to patients with AML on Day 0, dose assigned by age. If toxicity at Dose Level 4 would allow further escalation, but demonstrated sufficient AC220 activity, no further dose escalation will be required.

Group Type EXPERIMENTAL

AC220

Intervention Type DRUG

Dose assigned at study entry. AC220 will be given orally once daily on days 7-28.

Cytarabine

Intervention Type DRUG

All patients receive 1000 mg/m2/day IV given every 12 hours on days 1 through 5. Additionally, AML patients and patients with ambiguous leukemia receive cytarabine intrathecally on day "1" of course 1 and 2. Dose defined by age:

* 20 mg for patients age \<1 yr
* 30 mg for patients age 1-1.99 years of age
* 50 mg for patients age 2-2.99 years of age
* 70 mg for patients \>3 years of age

Etoposide

Intervention Type DRUG

150 mg/m2/day IV on days 1 through 5.

AML AC220 @ 130mg/m2/day (Dose Level 5)

If the study dose of 90 mg/m2/day at Dose Level 4 is well tolerated but does not show sufficient AC220 activity, the study may proceed to Dose Level 5 at 130 mg/m2/day. Patients will received etoposide and cytarabine on Days 1-5, and AC220 on Day 7 through 28. IT cytarabine will be given to patients with AML on Day 0, dose assigned by age. Dose Level 5 is the highest dose for this study.

Group Type EXPERIMENTAL

AC220

Intervention Type DRUG

Dose assigned at study entry. AC220 will be given orally once daily on days 7-28.

Cytarabine

Intervention Type DRUG

All patients receive 1000 mg/m2/day IV given every 12 hours on days 1 through 5. Additionally, AML patients and patients with ambiguous leukemia receive cytarabine intrathecally on day "1" of course 1 and 2. Dose defined by age:

* 20 mg for patients age \<1 yr
* 30 mg for patients age 1-1.99 years of age
* 50 mg for patients age 2-2.99 years of age
* 70 mg for patients \>3 years of age

Etoposide

Intervention Type DRUG

150 mg/m2/day IV on days 1 through 5.

Interventions

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AC220

Dose assigned at study entry. AC220 will be given orally once daily on days 7-28.

Intervention Type DRUG

Cytarabine

All patients receive 1000 mg/m2/day IV given every 12 hours on days 1 through 5. Additionally, AML patients and patients with ambiguous leukemia receive cytarabine intrathecally on day "1" of course 1 and 2. Dose defined by age:

* 20 mg for patients age \<1 yr
* 30 mg for patients age 1-1.99 years of age
* 50 mg for patients age 2-2.99 years of age
* 70 mg for patients \>3 years of age

Intervention Type DRUG

Etoposide

150 mg/m2/day IV on days 1 through 5.

Intervention Type DRUG

Methotrexate

IT methotrexate given intrathecally to patients with ALL on day "0" of course 1 and 2. Dose defined by age

* 6 mg for patients age \< 1yr
* 8 mg for patients age 1-1.99
* 10 mg for patients age 2-2.99
* 12 mg for patients 3-8.99 years of age
* 15 mg for patients \>9 years of age

Intervention Type DRUG

Other Intervention Names

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Quizartinib Cytosine Arabinoside Ara-C Cytosar®-U Arabinosylcytosine VePesid® Etopophos VP-16 Toposar® Etoposide phosphate MTX Amethopterin Otrexup™ Rasuvo® Rheumatrex® Trexall™ Methotrexate Sodium

Eligibility Criteria

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Inclusion Criteria

* Patients must be greater than 1 month and ≤ 21 years of age at study entry.
* Patients must have a diagnosis of relapsed/refractory AML, ALL or acute leukemia of ambiguous lineage and meet the following criteria:

1. Patients with AML or leukemia with ambiguous lineage must have greater than or equal to 5% blasts in the bone marrow.
2. Patients with ALL must have an M3 marrow (marrow blasts \>25%).
3. Patients with ALL must have MLL gene rearrangement or hyperdiploid \>50 chromosomes.
4. Patients with treatment related AML (t-AML) are eligible, provided they meet all other eligibility criteria.
* Karnofsky \> 50% for patients \>16 years of age and Lansky \>50% for patients ≤16 years of age.
* Patients must have fully recovered from the acute toxic effects of all prior chemotherapy, immunotherapy, or radiotherapy prior to entering this study.

* Myelosuppressive chemotherapy:

* Patients with ALL who relapse during standard maintenance therapy are eligible at time of relapse.
* For patients with ALL and AML who relapse while they are receiving cytotoxic therapy, at least 14 days must have elapsed since the completion of cytotoxic therapy.
* Cytoreduction with hydroxyurea can be initiated and continued for up to 24 hours prior to the start of AC220.
* Patients who have received other FLT3 inhibitors (ex. lestaurtinib, sorafenib) are eligible for this study.
* Hematopoietic growth factors: At least 7 days since the completion of therapy with a growth factor.
* Biologic (anti-neoplastic agent): At least 7 days since the completion of therapy with a biologic agent. For agents that have known adverse events occurring beyond 7 days after administration, this period must be extended beyond the time during which adverse events are known to occur. The duration of this interval must be discussed with the study chair.
* XRT: 2 wks must have elapsed since local palliative XRT for CNS chloromas; No washout period is necessary for other chloromas; at least 3 months must have elapsed if prior TBI, craniospinal XRT.
* Hematopoetic Stem Cell Transplant: At least 90 days must have elapsed since hematopoietic stem cell transplant (HSCT) and patients must not have active GVHD.
* Patient must have adequate renal and hepatic functions as indicated by the following laboratory values:

* Patients must have a calculated creatinine clearance or radioisotope GFR ≥70mL/min/1.73m2 or a normal serum creatinine based on age/gender.
* Total bilirubin \<1.5 x ULN for age or normal conjugated bilirubin.
* Alanine transaminase (ALT) \<5 × ULN (unless related to leukemic involvement).
* Patient must have a shortening fraction of ≥ 27% by echocardiogram, OR an ejection fraction of ≥ 50% by radionuclide angiogram.
* Reproductive Function

* Female patients of childbearing potential must have a negative urine or serum pregnancy test confirmed within 2 weeks prior to enrollment.
* Female patients with infants must agree not to breastfeed their infants while on this study.
* Male and female patients of child-bearing potential must agree to use an effective method of contraception approved by the investigator during the study and for a minimum of 6 months after study treatment.

Exclusion Criteria

* Patients will be excluded if they have CNS 3 disease.
* Patients will be excluded if they have uncontrolled or significant cardiovascular disease, including:

* A myocardial infarction within 12 months.
* Uncontrolled angina within 6 months.
* Diagnosed or suspected congenital long QT syndrome or any history of clinically significant ventricular arrhythmias (such as ventricular tachycardia, ventricular fibrillation, or torsades de pointes \[TdP\]); any history of arrhythmia will be discussed with the Sponsor's Medical Monitor prior to patient's entry into the study.
* Prolonged QTcF interval on pre-entry ECG (≥450 ms).
* Any history of second or third degree heart block (may be eligible if the patient currently has a pacemaker).
* Heart rate \< 50/minute on pre-entry ECG.
* Uncontrolled hypertension.
* Complete left bundle branch block.
* Any history of clinically significant ventricular arrhythmias (such as ventricular tachycardia, ventricular fibrillation, or TdP.
* Patients will be excluded if they have a systemic fungal, bacterial, viral or other infection that is exhibiting ongoing signs/symptoms related to the infection without improvement despite appropriate antibiotics or other treatment. The patient needs to be off pressors and have negative blood cultures for 48 hours.
* Patient is receiving or plans to receive concomitant chemotherapy, radiation therapy, or immunotherapy other than as specified in the protocol.
* Any significant concurrent disease, illness, psychiatric disorder or social issue that would compromise patient safety or compliance, interfere with consent, study participation, follow up, or interpretation of study results.
* Patients who are concurrently receiving CYP3A4 and 5 inhibitors and inducers
Minimum Eligible Age

1 Month

Maximum Eligible Age

21 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Ambit Biosciences Corporation

INDUSTRY

Sponsor Role collaborator

Therapeutic Advances in Childhood Leukemia Consortium

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Todd Cooper, MD

Role: STUDY_CHAIR

Children's Healthcare of Atlanta, Emory University

Locations

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Childrens Hospital Los Angeles

Los Angeles, California, United States

Site Status

Children's Hospital Central California

Madera, California, United States

Site Status

UCSF School of Medicine

San Francisco, California, United States

Site Status

The Children's Hospital, University of Colorado

Aurora, Colorado, United States

Site Status

Children's Healthcare of Atlanta, Emory University

Atlanta, Georgia, United States

Site Status

Johns Hopkins University

Baltimore, Maryland, United States

Site Status

Dana Farber

Boston, Massachusetts, United States

Site Status

Children's Mercy Hospitals and Clinics

Kansas City, Missouri, United States

Site Status

Levine Children's Hospital at Carolinas Medical Center

Charlotte, North Carolina, United States

Site Status

Oregon Health and Science University

Portland, Oregon, United States

Site Status

Seattle Children's Hospital

Seattle, Washington, United States

Site Status

Countries

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United States

References

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Cooper TM, Cassar J, Eckroth E, Malvar J, Sposto R, Gaynon P, Chang BH, Gore L, August K, Pollard JA, DuBois SG, Silverman LB, Oesterheld J, Gammon G, Magoon D, Annesley C, Brown PA. A Phase I Study of Quizartinib Combined with Chemotherapy in Relapsed Childhood Leukemia: A Therapeutic Advances in Childhood Leukemia & Lymphoma (TACL) Study. Clin Cancer Res. 2016 Aug 15;22(16):4014-22. doi: 10.1158/1078-0432.CCR-15-1998. Epub 2016 Feb 26.

Reference Type RESULT
PMID: 26920889 (View on PubMed)

Related Links

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http://www.tacl.us

Therapeutic Advances in Childhood Leukemia \& Lymphoma Consortium web site

Other Identifiers

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T2009-004

Identifier Type: -

Identifier Source: org_study_id

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