A Phase IIA Trial to Evaluate the Safety and Immunogenicity of a DNA HIV-1 Vaccine Followed by an MVA HIV-1 Vaccine in HIV-uninfected Volunteers

NCT ID: NCT01371175

Last Updated: 2011-06-10

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE2

Total Enrollment

115 participants

Study Classification

INTERVENTIONAL

Study Start Date

2003-04-30

Brief Summary

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The purpose of this study is to evaluate the safety and tolerability of plasmid DNA and recombinant MVA (Modified Vaccinia Virus Ankara) in a prime-boost regimen.

Approximately 111 volunteers (90 vaccine recipients/21 placebo recipients) will be enrolled at two sites. Approximately 56 volunteers will be enrolled at each site. An over-enrolment of up to 10% (approximately 10 additional volunteers) will be permitted in the study.

Detailed Description

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Conditions

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HIV Infections

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

PREVENTION

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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Group A

DNA or Placebo delivered intramuscularly at Months 0 and 1 followed by MVA or Placebo at Months 5 and 8 delivered subcutaneously. Vaccine:Placebo =12/3

Group Type EXPERIMENTAL

DNA.HIVA

Intervention Type BIOLOGICAL

0.5mg DNA.HIVA or placebo

MVA.HIVA

Intervention Type BIOLOGICAL

5x10\^6 pfu MVA or placebo

Group B

DNA or Placebo delivered intramuscularly at Months 0 and 1 followed by MVA or Placebo at Months 5 and 8 delivered intramuscularly. Vaccine:Placebo =12/3

Group Type EXPERIMENTAL

DNA.HIVA

Intervention Type BIOLOGICAL

0.5mg DNA.HIVA or placebo

MVA.HIVA

Intervention Type BIOLOGICAL

5x10\^6 pfu MVA or placebo

Group C

DNA or Placebo delivered intramuscularly at Months 0 and 1 followed by MVA or Placebo at Months 5 and 8 delivered intradermally. Vaccine:Placebo =18/3

Group Type EXPERIMENTAL

DNA.HIVA

Intervention Type BIOLOGICAL

0.5mg DNA.HIVA or placebo

MVA.HIVA

Intervention Type BIOLOGICAL

5x10\^7 pfu MVA or placebo

Group D

DNA or Placebo delivered intramuscularly at Months 0 and 1 followed by MVA or Placebo at Months 5 and 8 delivered subcutaneously. Vaccine:Placebo =12/3

Group Type EXPERIMENTAL

DNA.HIVA

Intervention Type BIOLOGICAL

0.5mg DNA.HIVA or placebo

MVA.HIVA

Intervention Type BIOLOGICAL

5x10\^7 pfu MVA or placebo

Group E

DNA or Placebo delivered intramuscularly at Months 0 and 1 followed by MVA or Placebo at Months 5 and 8 delivered intramuscularly. Vaccine:Placebo =12/3

Group Type EXPERIMENTAL

DNA.HIVA

Intervention Type BIOLOGICAL

0.5mg DNA.HIVA or placebo

MVA.HIVA

Intervention Type BIOLOGICAL

5x10\^7 pfu MVA or placebo

MVA.HIVA

Intervention Type BIOLOGICAL

2.5x10\^8 pfu MVA or placebo

Group F

DNA or Placebo delivered intramuscularly at Months 0 and 1 followed by MVA or Placebo at Months 5 and 8 delivered subcutaneously. Vaccine:Placebo =12/3

Group Type EXPERIMENTAL

DNA.HIVA

Intervention Type BIOLOGICAL

0.5mg DNA.HIVA or placebo

MVA.HIVA

Intervention Type BIOLOGICAL

2.5x10\^8 pfu MVA or placebo

Group G

DNA or Placebo delivered intramuscularly at Months 0 and 1 followed by MVA or Placebo at Months 5 and 8 delivered intramuscularly. Vaccine:Placebo =12/3

Group Type EXPERIMENTAL

DNA.HIVA

Intervention Type BIOLOGICAL

0.5mg DNA.HIVA or placebo

Groups C2/D2/E2 (Subgroups of C,D,E)

DNA or Placebo delivered intramuscularly at Months 0 and 1 followed by MVA or Placebo at Months 5 and Month 12+ (volunteers offered second MVA/placebo more than 12 months (late boost) after their enrollment into their original treatment assignment) delivered ID, SC, or IM according to original randomization. Vaccine:Placebo = blinded ratio, maximum in C2/D2/E2 = 16.

Group Type EXPERIMENTAL

DNA.HIVA

Intervention Type BIOLOGICAL

0.5mg DNA.HIVA or placebo

MVA.HIVA

Intervention Type BIOLOGICAL

5x10\^7 pfu MVA or placebo

Group F2/G2 (Subgroup of F and G)

DNA or Placebo delivered intramuscularly at Months 0 and 1 followed by MVA or Placebo at Months 5 and Month 12+ (volunteers offered second MVA/placebo more than 12 months (late boost) after their enrollment into their original treatment assignment) delivered either SC or IM according to original randomization. Vaccine:Placebo = blinded ratio, maximum in F/G= 29.

Group Type EXPERIMENTAL

DNA.HIVA

Intervention Type BIOLOGICAL

0.5mg DNA.HIVA or placebo

MVA.HIVA

Intervention Type BIOLOGICAL

2.5x10\^8 pfu MVA or placebo

Interventions

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DNA.HIVA

0.5mg DNA.HIVA or placebo

Intervention Type BIOLOGICAL

MVA.HIVA

5x10\^6 pfu MVA or placebo

Intervention Type BIOLOGICAL

MVA.HIVA

5x10\^7 pfu MVA or placebo

Intervention Type BIOLOGICAL

MVA.HIVA

2.5x10\^8 pfu MVA or placebo

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

1. Healthy males and females;
2. Age at least 18 years on the day of screening and no greater than 60 years on the day of enrolment;
3. Available for follow up for the planned duration of the study (screening plus 18 months);
4. Able to give written informed consent;
5. Does not engage in risk behaviour as defined by the protocol, willing to undergo HIV testing and receive results;
6. If sexually active female, using an effective method of contraception (combined oral contraceptive pill; injectable contraceptive; IUCD; condoms; anatomical sterility in self or partner) from screening until at least 4 months after last vaccination and willing to undergo urine pregnancy tests at screening and prior to each vaccination and 4 months after the last vaccination;
7. If sexually active male, willing to use an effective method of contraception (such as condoms) from screening until 4 months after the last vaccination.

Exclusion Criteria

1. Clinically relevant abnormality on history or examination including history of immunodeficiency or use of systemic corticosteroids, immunosuppressive, antiviral, anticancer, or other medications considered significant by the designated trial physician in last 6 months;
2. Presence of any chronic condition;
3. Any of the following abnormal laboratory parameters that are moderate, severe, or very severe: haematology (haemoglobin, absolute neutrophil count absolute lymphocyte count , absolute CD4 count, platelets); urinalysis, biochemistries (total bilirubin, creatinine, AST, ALT). Volunteers with mild laboratory abnormalities which are judged by the principal investigator or designee to be not clinically significant may be enrolled.
4. If female, pregnant or planning a pregnancy within 4 months after last vaccination or lactating;
5. Receipt of live attenuated vaccine within 60 days or other vaccine within 14 days of enrolment;
6. Receipt of blood transfusion or blood products 6 months prior to enrolment;
7. Participation in another clinical trial of an investigational product currently or within last 12 weeks or expected participation during this study;
8. History of severe local or general reaction to vaccination or history of allergic reactions;
9. History of grand-mal epilepsy, or currently taking anti-epileptics;
10. Confirmed HIV-1 or HIV-2 seropositive;
11. Positive for hepatitis B (surface antigen) or confirmed diagnosis of active syphilis at the time of enrolment (RPR positive and TPHA positive or equivalent), positive for hepatitis C antibodies;
12. Unlikely to comply with protocol. Prior receipt of smallpox vaccination should be documented, but will not be an exclusion criterion.
Minimum Eligible Age

18 Years

Maximum Eligible Age

60 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Medical Research Council-Oxford

UNKNOWN

Sponsor Role collaborator

University of Nairobi

OTHER

Sponsor Role collaborator

International AIDS Vaccine Initiative

NETWORK

Sponsor Role lead

Responsible Party

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International AIDS Vaccine Initiative

Principal Investigators

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Barry S. Peters, MD

Role: PRINCIPAL_INVESTIGATOR

Guys and St. Thomas' Hospital

Walter Jaoko

Role: PRINCIPAL_INVESTIGATOR

KAVI (Kenya AIDS Vaccine Initiative)

Locations

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KAVI (Kenya AIDS Vaccine Initiative)

Nairobi, , Kenya

Site Status

Guys and St. Thomas' Hospital

London, , United Kingdom

Site Status

Countries

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Kenya United Kingdom

Related Links

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http://www.iavi.org

International AIDS Vaccine Initiative

Other Identifiers

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IAVI 010

Identifier Type: -

Identifier Source: org_study_id

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