Biomarkers in Samples From Young Patients With Acute Myeloid Leukemia

NCT ID: NCT01247584

Last Updated: 2016-05-19

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

234 participants

Study Classification

OBSERVATIONAL

Study Start Date

2010-11-30

Brief Summary

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RATIONALE: Studying bone marrow samples from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research study is studying biomarkers in samples from young patients with acute myeloid leukemia.

Detailed Description

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OBJECTIVES:

* To compare the frequency of germline telomerase mutations in pediatric patients with acute myeloid leukemia (AML) demonstrating prolonged myelosuppression, defined as ≥ 1 episode \> 35 days of neutrophil count recovery after chemotherapy, to the pediatric patients with the expected myelosuppression, defined as consistently \< 35 days of neutrophil count recovery after chemotherapy.
* To assess association between telomerase mutations and incidence of grade 3 or 4 mucositis, relapse, and death.
* To compare germline (remission) telomere length in pediatric AML patients demonstrating delayed bone marrow recovery with the pediatric patients with consistently expected recovery.
* To assess whether a correlation between telomere length and incidence of grade 3 or 4 mucositis, relapse, and death exist.

OUTLINE: This is a multicenter study.

Cryopreserved bone marrow samples are analyzed for DNA sequencing and mutation by Sanger-based sequencing methods, quantitative PCR, and SeqMan Pro (Lasergene from DNAStar). Results are then compared with previously published data and existing databases to determine the allele frequency in control populations.

Conditions

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Leukemia

Study Design

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Observational Model Type

CASE_ONLY

Study Time Perspective

RETROSPECTIVE

Interventions

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DNA analysis

Intervention Type GENETIC

mutation analysis

Intervention Type GENETIC

polymerase chain reaction

Intervention Type GENETIC

laboratory biomarker analysis

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

DISEASE CHARACTERISTICS:

* Diagnosis of acute myeloid leukemia
* Enrolled on CCG-2961 and meeting 1 of the following criteria:

* More than 35 days to recover to an ANC \> 500/mm³ after any course of chemotherapy
* Consistently recovered \< 35 days to an ANC \> 500/mm³ after all courses of chemotherapy
* Available cryopreserved cell from diagnostic and end-of-therapy samples

PATIENT CHARACTERISTICS:

* Not specified

PRIOR CONCURRENT THERAPY:

* See Disease Characteristics
Maximum Eligible Age

21 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

Children's Oncology Group

NETWORK

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Maria M. Gramatges, MD

Role: PRINCIPAL_INVESTIGATOR

Baylor College of Medicine

Other Identifiers

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COG-AAML11B2

Identifier Type: OTHER

Identifier Source: secondary_id

NCI-2011-02846

Identifier Type: REGISTRY

Identifier Source: secondary_id

AAML11B2

Identifier Type: OTHER

Identifier Source: secondary_id

AAML11B2

Identifier Type: -

Identifier Source: org_study_id

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