Biomarkers in Tissue Samples From Patients With Acute Promyelocytic Leukemia

NCT ID: NCT01232855

Last Updated: 2015-10-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

10 participants

Study Classification

OBSERVATIONAL

Study Start Date

2010-11-30

Study Completion Date

2011-02-28

Brief Summary

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RATIONALE: Studying samples fo tissue from patients with cancer in the laboratory may help doctors identify and learn more about biomarkers related to cancer.

PURPOSE: This research study is studying biomarkers in tissue samples from patients with acute promyelocytic leukemia.

Detailed Description

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OBJECTIVES:

* Demonstrate that S100A10 is expressed on APL cells and that levels of S100A10 correspond to the amount of fibrinolytic activity.
* Evaluate the impact of ATRA on S100A10 level and fibrinolytic activity in vitro.
* Evaluate the impact of S100A10 knockdown on fibrinolytic activity.
* Correlate in vitro findings in cell culture to primary patient samples both pre- and post-ATRA therapy.

OUTLINE: This is a multicenter study.

Previously collected samples are analyzed via flow cytometry and western blot analysis. Cell surface levels of S100A10 and annexin A2 are correlated to the promyelocyte population and total protein levels are examined to determine total annexin A2 and S100A10 in acute promyelocytic leukemia cells.

Conditions

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Leukemia

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Interventions

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protein expression analysis

Intervention Type GENETIC

western blotting

Intervention Type GENETIC

flow cytometry

Intervention Type OTHER

laboratory biomarker analysis

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

DISEASE CHARACTERISTICS:

* Diagnosis of acute promyelocytic leukemia
* Available cryopreserved cells from diagnosis or cryopreserved cells from remission (germline)

PATIENT CHARACTERISTICS:

* Not specified

PRIOR CONCURRENT THERAPY:

* Not specified
Maximum Eligible Age

120 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

Children's Oncology Group

NETWORK

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Jason N. Berman, MD

Role: PRINCIPAL_INVESTIGATOR

IWK Health Centre

Other Identifiers

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COG-AAML11B5

Identifier Type: OTHER

Identifier Source: secondary_id

NCI-2011-02844

Identifier Type: REGISTRY

Identifier Source: secondary_id

AAML11B5

Identifier Type: OTHER

Identifier Source: secondary_id

AAML11B5

Identifier Type: -

Identifier Source: org_study_id

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