Mechanism of Aphakic and Pseudophakic Glaucoma in Pediatric Patients

NCT ID: NCT01136473

Last Updated: 2015-02-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

40 participants

Study Classification

OBSERVATIONAL

Study Start Date

2011-07-31

Study Completion Date

2014-09-30

Brief Summary

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In cell cultures, lens epithelial cells and trabecular meshwork cells will be exposed to aqueous humor from pediatric eyes undergoing cataract removal surgery. Cytokine analysis of candidate factors will be performed before and after exposure to lens epithelial.

Detailed Description

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Aqueous from the anterior chamber will be withdrawn before surgery. Then the lens capsule will be opened (capsulorhexis) and removed. The lens material will be removed. All 3 components will be sent to the lab.

Lens epithelial cells will be isolated and will be used in a co-culture setup with trabecular cells to investigate the interactions between lens cells and trabecular cells. Understanding the interaction between young and adult lens cells on trabecular cell will shed light on mechanism of infantile aphakic glaucoma.

Aqueous humor taken out before cataract surgery and before glaucoma operation of aphakic eyes will be used for analysis of growth factor and cytokines in the Aphakic eye using proteins membrane arrays. Eyes before cataract surgery will be used as controls. Such analysis will shed light on the possible factors affecting trabecular cells in aphakic eye.

Conditions

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Congenital Cataract Aphakic or Pseudophakic Glaucoma

Study Design

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Observational Model Type

CASE_ONLY

Study Time Perspective

CROSS_SECTIONAL

Study Groups

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congenital cataract or aphakic glaucoma

children with congenital cataract or aphakic glaucoma

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

* children with congenital cataract and are going to do removal surgery
* children with aphakic or pseudophakic glaucoma and are going to do glaucoma surgery
* both of parents are able to sign the INFORMED CONSENT FORM

Exclusion Criteria

* patients with other eye diseases apart from congenital cataract(in children with congenital cataract) and aphakic or pseudophakic glaucoma (in children with aphakic or pseudophakic glaucoma)
Maximum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Carmel Medical Center

OTHER

Sponsor Role lead

Responsible Party

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Orna Geyer

Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Orna Geyer, Prof

Role: PRINCIPAL_INVESTIGATOR

Carmel Medical Center

Locations

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Carmel Medical Center

Haifa, , Israel

Site Status

Countries

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Israel

Other Identifiers

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CMC-10-0035-CTIL

Identifier Type: -

Identifier Source: org_study_id

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