Observational Study on Anti-Tat Immune Response in HIV-1-infected HAART-treated Adult Subjects

NCT ID: NCT01024556

Last Updated: 2016-03-04

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

142 participants

Study Classification

OBSERVATIONAL

Study Start Date

2008-03-31

Study Completion Date

2012-02-29

Brief Summary

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The present study is designed as a prospective observational study directed at evaluating the frequency, magnitude, quality and persistence (primary endpoint) of the anti-Tat immune response in highly active antiretroviral therapy (HAART)-receiving HIV-1 infected individuals, and to prospectively evaluate the immunological, virological and clinical outcome of anti-Tat positive versus anti-Tat negative subjects under successful HAART (secondary endpoint), in order to determine the impact of anti-Tat immunity on HIV disease progression as well as the potential use of anti-Tat immune response assessment for the clinical and therapeutic management of HAART-treated infected patients. This survey provided important information for the design, planning and conduction of future therapeutic vaccine trials based on the HIV-1 Tat protein in HAART-treated patients.

Detailed Description

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Conditions

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HIV Infection

Study Design

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Study Time Perspective

PROSPECTIVE

Eligibility Criteria

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Inclusion Criteria

* Diagnosis of HIV-1 infection
* To be under successful HAART treatment with plasma viremia \<50 copies/ml in the last 6 months prior to initiation of the study, without a history of virologic rebound
* Known CD4+ T cells nadir
* Age ≥ 18 years old
* Signed informed consent

Exclusion Criteria

* Current therapy with immunomodulators or immunosuppressive drugs, or chemotherapy for neoplastic disorders
* Concomitant treatment for HBV or HCV infection
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Barbara Ensoli, MD

OTHER

Sponsor Role lead

Responsible Party

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Barbara Ensoli, MD

PHD

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Roberto Esposito, MD

Role: PRINCIPAL_INVESTIGATOR

Azienda Ospedaliero - Universitaria Policlinico di Modena, Modena, Italy

Giovanni Di Perri, MD

Role: PRINCIPAL_INVESTIGATOR

Amedeo di Savoia Hospital, Turin, Italy

Adriano Lazzarin, MD

Role: PRINCIPAL_INVESTIGATOR

San Raffaele Hospital - Milan, Italy

Massimo Galli, MD

Role: PRINCIPAL_INVESTIGATOR

L. Sacco Hospital- Milan, Italy

Giampiero Carosi, MD

Role: PRINCIPAL_INVESTIGATOR

"Spedali Civili" di Brescia , Brescia, Italy

Florio Ghinelli, MD

Role: PRINCIPAL_INVESTIGATOR

Azienda Ospedaliero - Universitaria di Ferrara, Ferrara, Italy

Francesco Mazzotta, MD

Role: PRINCIPAL_INVESTIGATOR

S. M. Annunziata Hospital, Florence, Italy

Guido Palamara, MD

Role: PRINCIPAL_INVESTIGATOR

S. Gallicano Hospital- Rome, Italy

Fabrizio Soscia, MD

Role: PRINCIPAL_INVESTIGATOR

S. M. Goretti Hospital- Latina, Italy

Giuseppe Pastore, MD

Role: PRINCIPAL_INVESTIGATOR

"Ospedale Policlinico Consorziale"-Bari, Italy

Locations

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S.M. Goretti Hospital

Latina, Rome, Italy

Site Status

General Hospital of Bari

Bari, , Italy

Site Status

Spedali Civili di Brescia

Brescia, , Italy

Site Status

General Hospital-University of Ferrara

Ferrara, , Italy

Site Status

A.M. Annunziata Hospital

Florence, , Italy

Site Status

L. Sacco Hospital

Milan, , Italy

Site Status

San Raffaele Hospital

Milan, , Italy

Site Status

General Hospital-University of Modena

Modena, , Italy

Site Status

San Gallicano Hospital

Rome, , Italy

Site Status

Amedeo di Savoia Hospital

Torino, , Italy

Site Status

Countries

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Italy

References

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Andre P, Groettrup M, Klenerman P, de Giuli R, Booth BL Jr, Cerundolo V, Bonneville M, Jotereau F, Zinkernagel RM, Lotteau V. An inhibitor of HIV-1 protease modulates proteasome activity, antigen presentation, and T cell responses. Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13120-4. doi: 10.1073/pnas.95.22.13120.

Reference Type BACKGROUND
PMID: 9789051 (View on PubMed)

Arya SK, Guo C, Josephs SF, Wong-Staal F. Trans-activator gene of human T-lymphotropic virus type III (HTLV-III). Science. 1985 Jul 5;229(4708):69-73. doi: 10.1126/science.2990040.

Reference Type BACKGROUND
PMID: 2990040 (View on PubMed)

Ensoli B, Barillari G, Salahuddin SZ, Gallo RC, Wong-Staal F. Tat protein of HIV-1 stimulates growth of cells derived from Kaposi's sarcoma lesions of AIDS patients. Nature. 1990 May 3;345(6270):84-6. doi: 10.1038/345084a0.

Reference Type BACKGROUND
PMID: 2184372 (View on PubMed)

Ensoli B, Buonaguro L, Barillari G, Fiorelli V, Gendelman R, Morgan RA, Wingfield P, Gallo RC. Release, uptake, and effects of extracellular human immunodeficiency virus type 1 Tat protein on cell growth and viral transactivation. J Virol. 1993 Jan;67(1):277-87. doi: 10.1128/JVI.67.1.277-287.1993.

Reference Type BACKGROUND
PMID: 8416373 (View on PubMed)

Ensoli B, Fiorelli V, Ensoli F, Cafaro A, Titti F, Butto S, Monini P, Magnani M, Caputo A, Garaci E. Candidate HIV-1 Tat vaccine development: from basic science to clinical trials. AIDS. 2006 Nov 28;20(18):2245-61. doi: 10.1097/QAD.0b013e3280112cd1. No abstract available.

Reference Type BACKGROUND
PMID: 17117011 (View on PubMed)

Monini P, Cafaro A, Srivastava IK, Moretti S, Sharma VA, Andreini C, Chiozzini C, Ferrantelli F, Cossut MR, Tripiciano A, Nappi F, Longo O, Bellino S, Picconi O, Fanales-Belasio E, Borsetti A, Toschi E, Schiavoni I, Bacigalupo I, Kan E, Sernicola L, Maggiorella MT, Montin K, Porcu M, Leone P, Leone P, Collacchi B, Palladino C, Ridolfi B, Falchi M, Macchia I, Ulmer JB, Butto S, Sgadari C, Magnani M, Federico MP, Titti F, Banci L, Dallocchio F, Rappuoli R, Ensoli F, Barnett SW, Garaci E, Ensoli B. HIV-1 tat promotes integrin-mediated HIV transmission to dendritic cells by binding Env spikes and competes neutralization by anti-HIV antibodies. PLoS One. 2012;7(11):e48781. doi: 10.1371/journal.pone.0048781. Epub 2012 Nov 13.

Reference Type BACKGROUND
PMID: 23152803 (View on PubMed)

Bellino S, Tripiciano A, Picconi O, Francavilla V, Longo O, Sgadari C, Paniccia G, Arancio A, Angarano G, Ladisa N, Lazzarin A, Tambussi G, Nozza S, Torti C, Foca E, Palamara G, Latini A, Sighinolfi L, Mazzotta F, Di Pietro M, Di Perri G, Bonora S, Mercurio VS, Mussini C, Gori A, Galli M, Monini P, Cafaro A, Ensoli F, Ensoli B. The presence of anti-Tat antibodies in HIV-infected individuals is associated with containment of CD4+ T-cell decay and viral load, and with delay of disease progression: results of a 3-year cohort study. Retrovirology. 2014 Jun 24;11:49. doi: 10.1186/1742-4690-11-49.

Reference Type BACKGROUND
PMID: 24961156 (View on PubMed)

Ensoli B, Cafaro A, Monini P, Marcotullio S, Ensoli F. Challenges in HIV Vaccine Research for Treatment and Prevention. Front Immunol. 2014 Sep 8;5:417. doi: 10.3389/fimmu.2014.00417. eCollection 2014.

Reference Type BACKGROUND
PMID: 25250026 (View on PubMed)

Ensoli F, Cafaro A, Casabianca A, Tripiciano A, Bellino S, Longo O, Francavilla V, Picconi O, Sgadari C, Moretti S, Cossut MR, Arancio A, Orlandi C, Sernicola L, Maggiorella MT, Paniccia G, Mussini C, Lazzarin A, Sighinolfi L, Palamara G, Gori A, Angarano G, Di Pietro M, Galli M, Mercurio VS, Castelli F, Di Perri G, Monini P, Magnani M, Garaci E, Ensoli B. HIV-1 Tat immunization restores immune homeostasis and attacks the HAART-resistant blood HIV DNA: results of a randomized phase II exploratory clinical trial. Retrovirology. 2015 Apr 29;12:33. doi: 10.1186/s12977-015-0151-y.

Reference Type BACKGROUND
PMID: 25924841 (View on PubMed)

Cafaro A, Tripiciano A, Sgadari C, Bellino S, Picconi O, Longo O, Francavilla V, Butto S, Titti F, Monini P, Ensoli F, Ensoli B. Development of a novel AIDS vaccine: the HIV-1 transactivator of transcription protein vaccine. Expert Opin Biol Ther. 2015;15 Suppl 1:S13-29. doi: 10.1517/14712598.2015.1021328. Epub 2015 Jun 22.

Reference Type BACKGROUND
PMID: 26096836 (View on PubMed)

Ensoli B, Bellino S, Tripiciano A, Longo O, Francavilla V, Marcotullio S, Cafaro A, Picconi O, Paniccia G, Scoglio A, Arancio A, Ariola C, Ruiz Alvarez MJ, Campagna M, Scaramuzzi D, Iori C, Esposito R, Mussini C, Ghinelli F, Sighinolfi L, Palamara G, Latini A, Angarano G, Ladisa N, Soscia F, Mercurio VS, Lazzarin A, Tambussi G, Visintini R, Mazzotta F, Di Pietro M, Galli M, Rusconi S, Carosi G, Torti C, Di Perri G, Bonora S, Ensoli F, Garaci E. Therapeutic immunization with HIV-1 Tat reduces immune activation and loss of regulatory T-cells and improves immune function in subjects on HAART. PLoS One. 2010 Nov 11;5(11):e13540. doi: 10.1371/journal.pone.0013540.

Reference Type RESULT
PMID: 21085635 (View on PubMed)

Tripiciano A, Picconi O, Moretti S, Sgadari C, Cafaro A, Francavilla V, Arancio A, Paniccia G, Campagna M, Pavone-Cossut MR, Sighinolfi L, Latini A, Mercurio VS, Pietro MD, Castelli F, Saracino A, Mussini C, Perri GD, Galli M, Nozza S, Ensoli F, Monini P, Ensoli B. Anti-Tat immunity defines CD4+ T-cell dynamics in people living with HIV on long-term cART. EBioMedicine. 2021 Apr;66:103306. doi: 10.1016/j.ebiom.2021.103306. Epub 2021 Apr 7.

Reference Type DERIVED
PMID: 33839064 (View on PubMed)

Related Links

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Other Identifiers

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ISS OBS T-002

Identifier Type: -

Identifier Source: org_study_id

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