Metabolome and Microbiomic in Fecal Samples

NCT ID: NCT00938028

Last Updated: 2009-07-21

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

4 participants

Study Classification

OBSERVATIONAL

Study Start Date

2009-04-30

Study Completion Date

2009-05-31

Brief Summary

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The purpose of this study is to evaluate the effects of storage and handling on fecal samples collected for metabolomic, proteomic and microbiomic and analysis.

Detailed Description

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Background

Human fecal collection for clinical or research studies usually occurs at the residence of the subject, an environment not equipped with measures to accommodate proper preparation and storage of samples for microbiome and metabolome analysis.

Aims

To evaluate the effects of storage and handling on fecal samples collected for metabolomic, proteomic and microbiomic and analysis.

Methods

Fecal samples will be collected within several minutes of voiding from healthy infants at the time of diaper change at day-care center.

Samples will be divided into 7 tubes, and subjected to different storage temperatures, before preforming microbial analysis by DNA sequencing, and metabolome analysis by H1NMR spectroscoty.

Conditions

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Infant

Study Design

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Study Time Perspective

PROSPECTIVE

Study Groups

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Microbiome, Metabolome, Stool, Toddler

healthy infant stool samples

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

* healthy
* age 12-20 months
Minimum Eligible Age

12 Months

Maximum Eligible Age

20 Months

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Massachusetts General Hospital

OTHER

Sponsor Role lead

Responsible Party

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MassGeneral Hospital for Children

Principal Investigators

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Harland Winter, MD

Role: PRINCIPAL_INVESTIGATOR

MGH

Locations

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MassGeneral for Children

Boston, Massachusetts, United States

Site Status

Countries

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United States

Other Identifiers

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2009p-000636

Identifier Type: -

Identifier Source: org_study_id

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