Dose Finding Study of a DNA Vaccine Delivered With Intradermal Electroporation in Patients With Prostate Cancer

NCT ID: NCT00859729

Last Updated: 2014-03-17

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

15 participants

Study Classification

INTERVENTIONAL

Study Start Date

2008-12-31

Study Completion Date

2011-11-30

Brief Summary

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This study will assess the feasibility and safety of vaccination with increasing doses of xenogenic DNA administered intradermally in combination with electroporation in patients with relapse of prostate cancer. The DNA encodes prostate specific antigen (PSA) from Rhesus Macaque (Macaca mulatta), a protein that is 89% homologous to human PSA. The study will also assess the safety and functionality of the DERMA VAX™ (Cyto Pulse Sciences) DNA vaccine delivery system.

Detailed Description

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Conditions

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Prostate Cancer

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Cohort I

50 µg DNA/dose, 3 patients

Group Type EXPERIMENTAL

pVAXrcPSAv53l (DNA encoding rhesus PSA)

Intervention Type BIOLOGICAL

5 doses, 4 weeks apart

DERMA VAX™ intradermal DNA delivery system

Intervention Type DEVICE

in vivo electroporation is applied after each DNA injection

Cohort II

150 µg DNA/dose, 3 patients

Group Type EXPERIMENTAL

pVAXrcPSAv53l (DNA encoding rhesus PSA)

Intervention Type BIOLOGICAL

5 doses, 4 weeks apart

DERMA VAX™ intradermal DNA delivery system

Intervention Type DEVICE

in vivo electroporation is applied after each DNA injection

Cohort III

400 µg DNA/dose, 3 patients

Group Type EXPERIMENTAL

pVAXrcPSAv53l (DNA encoding rhesus PSA)

Intervention Type BIOLOGICAL

5 doses, 4 weeks apart

DERMA VAX™ intradermal DNA delivery system

Intervention Type DEVICE

in vivo electroporation is applied after each DNA injection

Cohort IV

1000 µg DNA/dose, 3 patients

Group Type EXPERIMENTAL

pVAXrcPSAv53l (DNA encoding rhesus PSA)

Intervention Type BIOLOGICAL

5 doses, 4 weeks apart

DERMA VAX™ intradermal DNA delivery system

Intervention Type DEVICE

in vivo electroporation is applied after each DNA injection

Cohort V

Optimal dose to be determined, 6 patients

Group Type EXPERIMENTAL

pVAXrcPSAv53l (DNA encoding rhesus PSA)

Intervention Type BIOLOGICAL

5 doses, 4 weeks apart

DERMA VAX™ intradermal DNA delivery system

Intervention Type DEVICE

in vivo electroporation is applied after each DNA injection

Interventions

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pVAXrcPSAv53l (DNA encoding rhesus PSA)

5 doses, 4 weeks apart

Intervention Type BIOLOGICAL

DERMA VAX™ intradermal DNA delivery system

in vivo electroporation is applied after each DNA injection

Intervention Type DEVICE

Other Intervention Names

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rhPSA Derma Vax

Eligibility Criteria

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Inclusion Criteria

* Male patients. Age \>18 years.
* HLA-A\*0201 positive.
* Histologically confirmed prostate cancer.
* Minimum two (2) and maximum four (4) years after treatment with curative or salvage radiotherapy.
* Serum testosterone within normal range.
* Increasing PSA from a previous reference value on two (2) consecutive occasions at least one month apart and with a minimum of 2 ng/mL above nadir.
* PSA doubling time is one (1) year or less.
* No evidence of metastatic prostate cancer.
* Karnofsky performance status ≥ 80.
* Adequate organ function:

* AST and ALT ≤ 2.0 x upper limit of normal (ULN); total serum bilirubin ≤ 1.5 x ULN
* Calcium ≤ 2.6 mmol/L, serum creatinine ≤ 1.5 x ULN
* Hb ≥ 100 g/L; absolute leukocyte count ≥ 3.0 x 109 /L; platelets ≥100 x 109 /L
* Life expectancy ≥ 12 months.
* Swedish or English speaking subjects only.
* Written informed consent (subjects must be capable of providing their own informed consent).

Exclusion Criteria

* Previous ablation of testis.
* Radiologic evidence of metastatic disease.
* Prior chemotherapy or investigational therapy/agents within 4 weeks.
* Active bacterial, viral or fungal infection.
* Carrier of HIV, HBV, or HCV.
* Immunosuppressed (post splenectomy, post stem cell transplantation) or on immunosuppressive therapy other than inhaled or replacement corticosteroids.
* Any other major illness or peripheral blood vein status that, in the investigator's judgement, will substantially increase the risk associated with sampling or participation in this study.
* Subjects with cardiac demand pacemakers.
* Any reason why, in the opinion of the investigator, the patient should not participate.
Minimum Eligible Age

18 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

No

Sponsors

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Karolinska Institutet

OTHER

Sponsor Role collaborator

Cyto Pulse Sciences, Inc.

INDUSTRY

Sponsor Role collaborator

Uppsala University

OTHER

Sponsor Role lead

Responsible Party

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Jeffrey Yachnin

Prinicple Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Jeffrey Yachnin, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Department of Oncology, University Hospital Uppsala

Locations

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Department of Oncology, University Hospital Uppsala

Uppsala, , Sweden

Site Status

Countries

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Sweden

Other Identifiers

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EudraCT # 2006-001128-38

Identifier Type: -

Identifier Source: secondary_id

pVAX/rhPSA -EP 2006

Identifier Type: -

Identifier Source: org_study_id

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