Methylation of p16 CpG Island And Malignant Transformation of Oral Epithelial Dysplasia

NCT ID: NCT00835341

Last Updated: 2015-06-01

Study Results

Results available

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Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

93 participants

Study Classification

OBSERVATIONAL

Study Start Date

2005-03-31

Study Completion Date

2008-10-31

Brief Summary

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Oral epithelial dysplasia (OED) is one of the common precancerous lesions among Chinese adults. Biomarker is not available for detection of malignant potential of OED till now. p16 is an important tumor suppressor gene, which is inactivated frequently by methylation of CpG island in early stage of carcinogenesis. The present cohort study is to investigate whether p16 methylation is correlated with malignant transformation of OED.

Detailed Description

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* Background: Identification of malignant potential of oral epithelial dysplasia (OED) is virtually impossible on histopathological grounds alone. Inactivation of p16 gene by CpG methylation is an early frequent event during oral carcinogenesis. To investigate the predictive value of p16 methylation on malignant potential in OED, we carried out the prospective cohort study.
* Methods: 101 patients with histologically confirmed mild or moderate OED were included in the present study. Baseline information of p16 methylation status of the OED lesions from 93 cases was obtained by methylation-specific PCR. Progression of the OEDs lesions was examined in 78 cases histologically during the 45.8 months double-blind followup survey (78/93). The association between p16 methylation and progression of OED was analyzed with SPSS13.0 software. All P-values were two-sided.

Conditions

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Oral Epithelial Dysplasia, Mild or Moderate Grade

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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p16-methylated

patients with mild or moderate oral epithelial dysplasia containing methylated p16 CpG island.

No interventions assigned to this group

p16-unmethylated

patients with mild or moderate oral epithelial dysplasia NOT containing methylated p16 CpG island.

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

* histological diagnosis of mild or moderate grade OED; and
* enough amount of tissue sample from OED lesion for genomic DNA extraction; and
* available of methylation status of p16 CpG island in the extracted DNA sample.

Exclusion Criteria

* histological diagnosis of severe grade OED or malignant disease; or
* amount of tissue sample is not enough for preparation of genomic DNA (20ng); or
* quality of the prepared DNA is not good enough for detection of p16 methylation; or
* OED treatment history by LASER, radiotherapy, or chemotherapy
Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Peking University

OTHER

Sponsor Role lead

Responsible Party

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Dajun Deng

Professor and Department Director

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Dajun Deng, MD

Role: STUDY_DIRECTOR

Beijing Cancer Hospital/ Institue, Peking University School of Oncology

Hongwei Liu, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Peking University School of Stomatology

Locations

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Department of Oral Medicine, Peking University School and Hospital of Stomatology

Beijing, , China

Site Status

Countries

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China

References

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Gale N, Westra W, Pilch BZ, et al. Epithelial precursors lesions. In: Barnes L, Eveson JW, Reichart P, et al. eds. World Health Organization Classification of Tumors: Pathology and Genetics of Head and Neck Tumors. IARC Press, Lyon (France); 2005: 177-179.

Reference Type BACKGROUND

Sun Y, Deng D, You WC, Bai H, Zhang L, Zhou J, Shen L, Ma JL, Xie YQ, Li JY. Methylation of p16 CpG islands associated with malignant transformation of gastric dysplasia in a population-based study. Clin Cancer Res. 2004 Aug 1;10(15):5087-93. doi: 10.1158/1078-0432.CCR-03-0622.

Reference Type BACKGROUND
PMID: 15297411 (View on PubMed)

Cao J, Zhou J, Gao Y, Gu L, Meng H, Liu H, Deng D. Methylation of p16 CpG island associated with malignant progression of oral epithelial dysplasia: a prospective cohort study. Clin Cancer Res. 2009 Aug 15;15(16):5178-83. doi: 10.1158/1078-0432.CCR-09-0580. Epub 2009 Aug 11.

Reference Type RESULT
PMID: 19671846 (View on PubMed)

Related Links

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http://www.bjcancer.org

Beijing Cancer Hospital/ Institute, Peking University School of Oncology

http://ss.bjmu.edu.cn

Peking University School of Stomatology

Other Identifiers

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CPDHS-434

Identifier Type: -

Identifier Source: org_study_id

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