Generation of Dendritic Cell Precursors From Cord Blood Stem Cells
NCT ID: NCT00731744
Last Updated: 2008-08-11
Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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UNKNOWN
NA
50 participants
INTERVENTIONAL
2007-01-31
2010-12-31
Brief Summary
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We have established a serious of functional analysis of maturated or immaturated DCs including surface markers screening or cytokine production. Here, we design this two-year project to evaluate the possible methodology in the generation of CD14+CD1a- and CD14- CD1a+ myeloid DC precursors from CD34+ cells. In the first year of the project, we will set up the standard protocol to generate DC precursors from cord blood stem cells. In the first step, we will isolate CD34+ cells from cord blood and amplify these cells. Purity of the CD34+ cell populations obtained will be greater than 90%. In the next step, these primary cultures of CD34+ cells will treat with early-acting cytokines such as GM-CSF and IL-4 to further induction into dendritic cells. Functional analysis of dendritic cells would be performed in this stage. In the second year of the project, we will further evaluate the induction of DCs by CD34+ stem cells could be affected by combination of mesenchymal stromal cells. These results of this research with CD34+-based DCs induction provide the basis for materials to develop the DC-based vaccine against viral and fungal infections, and might be a powerful tool in anti-tumor immunotherapy.
Detailed Description
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Conditions
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Study Design
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NA
SINGLE_GROUP
BASIC_SCIENCE
NONE
Interventions
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Normal full-term deliveries
Normal full-term deliveries by standard procedures according to institutional guidelines
Eligibility Criteria
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Inclusion Criteria
25 Years
45 Years
FEMALE
Yes
Sponsors
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National Taiwan University Hospital
OTHER
Responsible Party
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National Taiwan Unviersity Hospital
Locations
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National Taiwan University Hospital
Taipei, , Taiwan
Countries
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Central Contacts
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Facility Contacts
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Chien-Nan Lee, ASSOCIATE PROFESSOR
Role: primary
Other Identifiers
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200706003R
Identifier Type: -
Identifier Source: org_study_id