Immunoregulation by Controlled Parasite Exposure in Multiple Sclerosis.

NCT ID: NCT00630383

Last Updated: 2012-06-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

WITHDRAWN

Clinical Phase

PHASE2

Study Classification

INTERVENTIONAL

Study Start Date

2008-02-29

Study Completion Date

2008-05-31

Brief Summary

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The aim of the study is to determine whether controlled infection with a clinically safe number of larvae of hookworm results in an immune response that is protective in relapsing MS.

Detailed Description

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Studies have shown that there may be an inverse relationship between infections with worms including hookworms and inflammatory diseases including multiple sclerosis (MS). This has been explained by a protective immune reaction that is triggered by the hookworm in the body that dampens inflammation. In mice with MS, infections with some mouse worms reduced the inflammation and damage to their brain. The primary purpose of this study is to determine whether people with MS who are exposed to a small number of hookworms will develop this protective immune reaction that may reduce MS disease activity. We also plan to determine the effect of the hookworms on relapses during 1 year study.

A study of people with MS naturally infected with intestinal parasites did show significant protection over 5 years, and the levels of biological markers of the infection and some immune substances triggered by it were similar to the ones we obtained with controlled infection in normal volunteers, allergic and asthmatic peoples. We think the study has a genuine potential to benefit people with MS, and there is known interest in the MS patient community. At the therapeutic doses proposed here, this is an innocuous infection. Natural hookworm infection affects 1 billion people worldwide, often without symptoms unless the parasite load is very high. Our controlled exposure studies have shown good tolerability and safety; the risk of infecting others and auto-infection virtually is nil in Western standard hygiene conditions. Many people with MS when asked stated they would prefer an innocuous infection with microscopic larvae to a man-made product that may have more side effects. If the protective mechanisms are determined these studies may also lead to new ways of treating MS, possibly by selecting only the specific chemical components of the worms and the immune response to them that confer protection.

The increase in MS in the Western world, along with other autoimmune inflammatory diseases and asthma may be attributed to decreased exposure to infections such as gut parasites due to improved hygiene ('the hygiene hypothesis'). In animal models, controlled parasite infections including hookworms and related worms protect against MS-like disease. Parasites have evolved host-specific molecular mechanisms to dampen or condition the excessive immune responses against them and thus survive. These parasites induce regulatory mechanisms including Treg and a novel class of B cells that also dampen immune responses called Breg and were recently shown to improve MS in natural infection. They may suppress a class of lymphocytes that cause most damage in MS, Th17 cells. We will produce, with controlled exposure, a similar response to those associated with protective natural exposure in MS. We have the unique combination of expertise in hookworm biology, controlled parasite exposure and immunology of MS and MS trials and our data from our other human studies indicate this is a safe and tolerable intervention of significant potential.

Conditions

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Multiple Sclerosis

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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1

Patients will receive 25 live hookworm larvae.

Group Type EXPERIMENTAL

Live Hookworm Larvae

Intervention Type BIOLOGICAL

25 live hookworm will be applied to the arm and will infect transdermally. They will be eradicated after 48 weeks.

2

Patients will receive 0.01 % histamine solution.

Group Type PLACEBO_COMPARATOR

Histamine

Intervention Type OTHER

0.01% histamine solution is pipetted onto a plaster dressing.

Interventions

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Live Hookworm Larvae

25 live hookworm will be applied to the arm and will infect transdermally. They will be eradicated after 48 weeks.

Intervention Type BIOLOGICAL

Histamine

0.01% histamine solution is pipetted onto a plaster dressing.

Intervention Type OTHER

Other Intervention Names

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Hookworm Necator Americanus

Eligibility Criteria

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Inclusion Criteria

1. Patients with documented multiple sclerosis relapsing remitting or secondary progressive with relapses, according to McDonald's criteria, and an MRI scan consistent with MS according to Fazekas criteria
2. Patients with at least 1 relapse in the last 12 months
3. Patients with EDSS score in the range of 0 to 5.5 at the baseline visit
4. Patients of both genders, age \>18 years and \< 60 years
5. Women of child bearing potential, (who have a negative pregnancy test) must agree to use methods of medically acceptable forms of contraception during the study.
6. Be able and willing to comply with study visits and procedures per protocol.
7. Understand, sign, and date the written voluntary informed consent form at the screening visit prior to any protocol-specific procedures performed.

Exclusion Criteria

No populations at risk of severe illness or death will be included in this study

1. Life expectancy \< 6 months.
2. Patient is \< 5 years free of malignancy, except treated basal cell skin cancer or cervical carcinoma in situ.
3. Patient with grade III/IV cardiac problems as defined by the New York Heart Association Criteria. (i.e., congestive heart failure, myocardial infarction within 6 months of study)
4. Patients with severe and/or uncontrolled medical condition.
5. Patient has a known diagnosis of human immunodeficiency virus (HIV) infection.
6. Anaemia (Hb \<10 g/dL for females, \<11 g/dL for males)
7. Prior or present evidence of parasitic infection; prior treatment with anti-helminthic drugs
8. Patient with serious medical or psychiatric illness that could potentially interfere with the completion of the study treatment according to this protocol
9. History of poor compliance or history of drug/alcohol abuse, or excessive alcohol consumption that would interfere with the ability to comply with the study protocol,
10. Severe asthma, allergy, other autoimmune disease or any condition that the physician judges could be detrimental to subjects participating in this study; including deviations deemed clinically important from normal clinical laboratory

Previous treatment

1. Treatment with interferon or glatiramer acetate or immunosuppressive drugs within 26 weeks prior to baseline
2. Treatment with bone marrow transplantation, total lymphoid irradiation, monoclonal antibodies, umbilical cord stem cells, AIMSPRO at any time prior to baseline
3. Treatment with corticosteroids or ACTH within 4 weeks prior to baseline
4. Treatment with any investigational agent within 12 weeks prior to baseline
Minimum Eligible Age

18 Years

Maximum Eligible Age

60 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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University of Nottingham

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Cris Constantinescu, MD PhD

Role: PRINCIPAL_INVESTIGATOR

University of Nottingham

Locations

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Nottingham University Hospital NHS Trust

Nottingham, Nottinghamshire, United Kingdom

Site Status

Countries

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United Kingdom

References

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Bach JF. The effect of infections on susceptibility to autoimmune and allergic diseases. N Engl J Med. 2002 Sep 19;347(12):911-20. doi: 10.1056/NEJMra020100. No abstract available.

Reference Type BACKGROUND
PMID: 12239261 (View on PubMed)

Edwards LJ, Constantinescu CS. A prospective study of conditions associated with multiple sclerosis in a cohort of 658 consecutive outpatients attending a multiple sclerosis clinic. Mult Scler. 2004 Oct;10(5):575-81. doi: 10.1191/1352458504ms1087oa.

Reference Type BACKGROUND
PMID: 15471376 (View on PubMed)

Steinman L. A brief history of T(H)17, the first major revision in the T(H)1/T(H)2 hypothesis of T cell-mediated tissue damage. Nat Med. 2007 Feb;13(2):139-45. doi: 10.1038/nm1551.

Reference Type BACKGROUND
PMID: 17290272 (View on PubMed)

Viglietta V, Baecher-Allan C, Weiner HL, Hafler DA. Loss of functional suppression by CD4+CD25+ regulatory T cells in patients with multiple sclerosis. J Exp Med. 2004 Apr 5;199(7):971-9. doi: 10.1084/jem.20031579.

Reference Type BACKGROUND
PMID: 15067033 (View on PubMed)

Fleming JO, Cook TD. Multiple sclerosis and the hygiene hypothesis. Neurology. 2006 Dec 12;67(11):2085-6. doi: 10.1212/01.wnl.0000247663.40297.2d. No abstract available.

Reference Type BACKGROUND
PMID: 17159130 (View on PubMed)

Correale J, Farez M. Association between parasite infection and immune responses in multiple sclerosis. Ann Neurol. 2007 Feb;61(2):97-108. doi: 10.1002/ana.21067.

Reference Type BACKGROUND
PMID: 17230481 (View on PubMed)

Sewell D, Qing Z, Reinke E, Elliot D, Weinstock J, Sandor M, Fabry Z. Immunomodulation of experimental autoimmune encephalomyelitis by helminth ova immunization. Int Immunol. 2003 Jan;15(1):59-69. doi: 10.1093/intimm/dxg012.

Reference Type BACKGROUND
PMID: 12502726 (View on PubMed)

Mortimer K, Brown A, Feary J, Jagger C, Lewis S, Antoniak M, Pritchard D, Britton J. Dose-ranging study for trials of therapeutic infection with Necator americanus in humans. Am J Trop Med Hyg. 2006 Nov;75(5):914-20.

Reference Type BACKGROUND
PMID: 17123987 (View on PubMed)

Compston A, Coles A. Multiple sclerosis. Lancet. 2002 Apr 6;359(9313):1221-31. doi: 10.1016/S0140-6736(02)08220-X.

Reference Type BACKGROUND
PMID: 11955556 (View on PubMed)

Hotez PJ, Pritchard DI. Hookworm infection. Sci Am. 1995 Jun;272(6):68-74. doi: 10.1038/scientificamerican0695-68.

Reference Type BACKGROUND
PMID: 7761817 (View on PubMed)

Falcone FH, Pritchard DI. Parasite role reversal: worms on trial. Trends Parasitol. 2005 Apr;21(4):157-60. doi: 10.1016/j.pt.2005.02.002.

Reference Type BACKGROUND
PMID: 15780835 (View on PubMed)

Raine T, Zaccone P, Dunne DW, Cooke A. Can helminth antigens be exploited therapeutically to downregulate pathological Th1 responses? Curr Opin Investig Drugs. 2004 Nov;5(11):1184-91.

Reference Type BACKGROUND
PMID: 15573869 (View on PubMed)

Quinnell RJ, Bethony J, Pritchard DI. The immunoepidemiology of human hookworm infection. Parasite Immunol. 2004 Nov-Dec;26(11-12):443-54. doi: 10.1111/j.0141-9838.2004.00727.x.

Reference Type BACKGROUND
PMID: 15771680 (View on PubMed)

Other Identifiers

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WIRMS-Pilot

Identifier Type: -

Identifier Source: secondary_id

08025

Identifier Type: -

Identifier Source: org_study_id

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