Gene Expression Profiling of Cervical Cancer Progression in Biopsies and Tissue Samples

NCT ID: NCT00629577

Last Updated: 2017-02-01

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

TERMINATED

Clinical Phase

PHASE1

Total Enrollment

50 participants

Study Classification

INTERVENTIONAL

Study Start Date

2006-09-30

Study Completion Date

2011-12-31

Brief Summary

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RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research trial is studying gene expression profiling of cervical cancer progression in biopsies and tissue samples from patients with cervical lesions.

Detailed Description

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OBJECTIVES:

* Classify cervical cancer progression at a molecular level using gene expression profiling generated from expression microarrays.
* Profile gene expression changes of dysplasia and early carcinoma of uterine cervical tissue at a molecular level using expression microarrays.
* Define a set of genes that classify the various grades of cervical cancer/dysplasia at a molecular level.
* Compare the expression profiles of clinical data to determine how accurately cell lines model the in vivo environment.

OUTLINE: Patients undergo colposcopy and a cervical smear is taken for flow cytometry analysis. Patients also undergo 2 biopsies from one clinically abnormal or normal site. Tissue samples are analyzed by gene expression profiling using human expression microarrays containing approximately 40,000 unique sequences. Data analysis consists of defining a set of genes, containing new targets for biomarkers, that classify the biopsies into 3 grades.

Conditions

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Cervical Cancer Precancerous Condition

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Interventions

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microarray analysis

Intervention Type GENETIC

flow cytometry

Intervention Type OTHER

biopsy

Intervention Type PROCEDURE

colposcopy

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Included subjects will be ≥18 years old.
* Included subjects will not be pregnant.
* Included subjects will have a negative urine pregnancy test.
* Included subjects will be scheduled for colposcopy and/or LEEP treatment at the VGH Women's Clinic.
* Included subjects will indicate understanding of the study.
* Included subjects will provide informed consent to participate.

Exclusion Criteria

* Individuals \<18 years old will be excluded.
* Pregnant individuals will be excluded.
* Individuals that have had an operation to remove their cervix will be excluded.
Minimum Eligible Age

19 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

British Columbia Cancer Agency

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Thomas G Ehlen, M.D.

Role: PRINCIPAL_INVESTIGATOR

BC Cancer Agency, Vancouver General Hospital

Locations

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BC Cancer Research Centre

Vancouver, British Columbia, Canada

Site Status

Countries

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Canada

Other Identifiers

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R01CA103830

Identifier Type: NIH

Identifier Source: secondary_id

View Link

BCCR-H05-60100

Identifier Type: OTHER

Identifier Source: secondary_id

CDR0000581308

Identifier Type: -

Identifier Source: org_study_id

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