Evaluating the Use of RFT5-dgA to Deplete Alloreactive Cells Prior to Haploidentical Stem Cell Transplantation

NCT ID: NCT00586547

Last Updated: 2020-01-21

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: PHASE1
• Total Enrollment: 11 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2000-07-31
• Study Completion Date: 2008-09-30

Brief Summary

This study is designed to determine the number of donor lymphocytes that can be given to recipients of haploidentical stem cell transplants after depletion of recipient-reactive T lymphocytes by ex-vivo treatment with a fixed dose of RFT5-dgA immunotoxin, and will result in a rate of Grade III/IV GVHD of < / = 25%, to analyze immune reconstitution in these patients, and to measure their overall and disease free survival, at 100 days and at 1 year.

Detailed Description

Patients will receive cytosine arabinoside (3g/m^2) IV every 12 hours for 6 doses starting at 1400 hours on day -8. Cyclophosphamide (45mg/kg) will be given on Day -7 and Day -6. MESNA (45mg/kg, divided into 5 doses) will be administered 15 minutes prior to each dose of cyclophosphamide and 3, 6, 9 and12 hours after each dose of cyclophosphamide. Campath 1H IV will be given on Days -3, -2 and -1. TBI, total dose 14.0 Gy will be delivered in 8 fractions of 1.75 Gy in two fractions per day beginning Day -4. The dose rate will be 10cGy/min.

Approximately thirty days following transplantation (day +30), the cryopreserved T cells will be thawed and infused through a catheter line with normal saline.

This study will begin with a dose of T cells known not to cause GvHD even in haploidentical recipients, even when the T cells administered have not first been allodepleted. A subset of patients who achieved engraftment will be included in the dose escalation study of allodepleted T-cells treated with RFT5-dgA. A continual reassessment method based on a logistic dose-response curve with cohorts of size 2 will be employed to determine the MTD. Cohorts of size 2 will be accrued beginning at dose level 1 and the dose-response curve is estimated after toxicity outcome is observed to determine the recommended dose level for the next patient cohort. Each and every patient will receive up to five additional injections of T cells at the same dose, at monthly intervals, provided there is no evidence of grade 2 or higher GVHD, until total T cell numbers are > 1000/ul

Patients will be entered starting at level 1, according to the following doses:

Dose level -1 (1 x 10^3 T cells/Kg); Dose level 1 (1 x 10^4 T cells/Kg); Dose level 2 (1 x 10^5 T cells/Kg); Dose level 3 (1 x 10^6 T cells/Kg); Dose level 4 (5 x 10^6 T cells/Kg).

Conditions

Leukemia; Cancer

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Treatment

After patients have completed preparation to receive cells, they will be treated at one of five dose levels.

Group Type: EXPERIMENTAL

T-Cell Infusion Dose Level -1

Intervention Type: BIOLOGICAL

(1 x 10\^3 T cells/Kg)

T-Cell Infusion Dose Level 1

Intervention Type: BIOLOGICAL

(1 x 10\^4 T cells/Kg)

T-Cell Infusion Dose Level 2

Intervention Type: BIOLOGICAL

(1 x 10\^5 T cells/Kg)

T-Cell Infusion Dose Level 3

Intervention Type: BIOLOGICAL

(1 x 10\^6 T cells/Kg)

T-Cell Infusion- Dose Level 4

Intervention Type: BIOLOGICAL

(5 x 10\^6 T cells/Kg)

Interventions

T-Cell Infusion Dose Level -1

(1 x 10\^3 T cells/Kg)

Intervention Type: BIOLOGICAL

T-Cell Infusion Dose Level 1

(1 x 10\^4 T cells/Kg)

Intervention Type: BIOLOGICAL

T-Cell Infusion Dose Level 2

(1 x 10\^5 T cells/Kg)

Intervention Type: BIOLOGICAL

T-Cell Infusion Dose Level 3

(1 x 10\^6 T cells/Kg)

Intervention Type: BIOLOGICAL

T-Cell Infusion- Dose Level 4

(5 x 10\^6 T cells/Kg)

Intervention Type: BIOLOGICAL

Eligibility Criteria

Inclusion Criteria

• ALL or high grade NHL that is Stage III or IV and has relapsed or is considered to be primary refractory disease.
• Myelodysplastic syndrome.
• AML after first relapse or with primary refractory disease.
• CML hemophagocytic lymphohistiocytosis (HLH)
• Familial hemophagocytic lymphohistiocytosis (FLH)
• Viral-associated hemophagocytic syndrome (VAHS)
• X-linked lymphoproliferative disease (XLP)
• Patients with Severe chronic active Epstein Barr virus infection (SCAEBV) with predilection for T- or NK-cell malignancy
• Lack of suitable conventional donor (i.e. 5/6 or 6/6 related or 5/6 or 6/6 unrelated donor) or presence of a rapidly progressive disease not permitting time to identify an unrelated donor.
• Donor cells should be collected and frozen before conditioning starts.

Exclusion Criteria

• Patients with a life expectancy (< or = to 6 weeks) limited by diseases other than leukemia.
• Patients with symptomatic cardiac disease, or evidence of significant cardiac disease by echocardiogram (i.e. shortening fraction < 25%)
• Patients with severe renal disease (i.e. creatinine clearance less than 40cc/1.73m2)
• Patients with pre-existing severe restrictive pulmonary disease (FVC less than 40% of predicted)
• Patients with severe hepatic disease (direct bilirubin greater than 3ug/dl or SGPT greater than 500ug/dl)
• Patients with severe personality disorder or mental illness that would preclude compliance with the study
• Patients with a severe infection that on evaluation by the Principal Investigator precluded ablative chemotherapy or successful transplantation
• Patients with documented HIV positivity

• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

University of Texas, Southwestern Medical Center at Dallas

OTHER

Sponsor Role: collaborator

Baylor College of Medicine

OTHER

Sponsor Role: lead

Responsible Party

Malcolm Brenner

Dist Serv Prof, Center for Gene Therapy

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Malcolm Brenner, MB, PhD

Role: PRINCIPAL_INVESTIGATOR

Baylor College of Medicine

Locations

Methodist Hospital

Houston, Texas, United States

Texas Children's Hospital

Houston, Texas, United States

Countries

United States

Other Identifiers

H-9033

Identifier Type: -

Identifier Source: org_study_id

NCT00622297

Identifier Type: -

Identifier Source: nct_alias