Synovial Fluid Bank From Arthritic Patients

NCT ID: NCT00512343

Last Updated: 2025-03-25

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

500 participants

Study Classification

OBSERVATIONAL

Study Start Date

2002-06-30

Study Completion Date

2030-09-30

Brief Summary

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The objectives of this group project is to collect synovial fluid (SF) and paired sera and carry out preclinical studies to evaluate, alone or by combination testings, the potential of novel biomarkers for their prevalence and diagnostic/prognostic values in patients with arthritis, and to determine how therapeutic intervention regulate their levels.

Detailed Description

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Our group has developed interdisciplinary expertise in biomarkers related to distinct aspects of inflammation-induced joint damage, most notably those associated with degradation of components of the extracellular matrix, bone loss, or associated with post-translational changes on protease inhibitors.

The objectives of this group project is to collect SF and paired sera and carry out preclinical studies to evaluate, alone or by combination testings, the potential of novel biomarkers for their prevalence and diagnostic/prognostic values in patients with arthritis, and to determine how therapeutic intervention regulate their levels.

More specifically, we will study (1) biomarkers associated with osteoclastic bone resorption and synthesis/degradation of cartilage, (2) inhibitors of enzymes (calpastatin) that are preferentially targeted by rheumatoid patients, as well as (3) enzymes (e.g. Matrix Metalloproteinases (MMPs)) responsible for the cleavage of components of the articular cartilage.

Finally, we propose to characterize the repertoire of specific active proteases in the SF of arthritic patients concomitantly to the use biomarkers (neoepitopes of cleaved collagens) previously associated with cartilage destruction and synthesis to identify new targets for the development of protease inhibitors for the treatment of arthritis. Furthermore, our project will also provide the opportunity to test the potential of measuring the net proteolytic activity by Fluorescent-Activated Substrate Conversion as a method of monitoring disease activity and treatment efficiency in a clinical setting.

Conditions

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Arthritis

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Eligibility Criteria

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Inclusion Criteria

* Significant joint effusion

Exclusion Criteria

* Hemorrhagic effusion
* Traumatic effusion
* Less than 3 ml total of collected fluid (used for routine analysis and culture)
* Inability or refusal to consent
Minimum Eligible Age

3 Years

Maximum Eligible Age

95 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Canadian Arthritis Network

NETWORK

Sponsor Role collaborator

Gilles Boire

OTHER

Sponsor Role lead

Responsible Party

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Gilles Boire

MD

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Gilles Boire, MD, MSc

Role: PRINCIPAL_INVESTIGATOR

CHUS and Université de Sherbrooke

Locations

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Centre hospitalier universitaire de Sherbrooke

Sherbrooke, Quebec, Canada

Site Status RECRUITING

Countries

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Canada

Central Contacts

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Gilles Boire, MD, MSc

Role: CONTACT

(819) 564-5261

Facility Contacts

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Gilles Boire, MD, MSc

Role: primary

(819) 564-5261

References

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Kilani RT, Maksymowych WP, Aitken A, Boire G, St-Pierre Y, Li Y, Ghahary A. Detection of high levels of 2 specific isoforms of 14-3-3 proteins in synovial fluid from patients with joint inflammation. J Rheumatol. 2007 Aug;34(8):1650-7. Epub 2007 Jul 1.

Reference Type RESULT
PMID: 17611984 (View on PubMed)

Simard N, Boire G, de Brum-Fernandes AJ, St-Pierre Y. A novel approach to measure the contribution of matrix metalloproteinase in the overall net proteolytic activity present in synovial fluids of patients with arthritis. Arthritis Res Ther. 2006;8(4):R125. doi: 10.1186/ar2014.

Reference Type RESULT
PMID: 16859524 (View on PubMed)

Other Identifiers

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02-12 LS

Identifier Type: -

Identifier Source: org_study_id

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