Study of Ductal Lavage in Women at High Risk for Breast Cancer

NCT ID: NCT00429988

Last Updated: 2012-09-26

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

30 participants

Study Classification

OBSERVATIONAL

Study Start Date

2002-08-31

Study Completion Date

2004-06-30

Brief Summary

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RATIONALE: Collecting samples of fluid using ductal lavage and nipple aspiration from participants at high risk for breast cancer to study in the laboratory may help doctors learn more about changes that may occur in DNA and identify biomarkers related to cancer.

PURPOSE: This clinical trial is using ductal lavage to collect fluid from women at high risk for breast cancer.

Detailed Description

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OBJECTIVES:

Primary

* Determine the volume of atypical cells collected from ductal lavage in women at increased risk for breast cancer.

Secondary

* Determine assay techniques to analyze nipple aspirate fluid and ductal lavage fluid samples from these participants.
* Analyze the nipple aspirate fluid from these participants for specific and global protein signatures.
* Determine the replicability of markers in serial assays of these participants.
* Determine the percentage of participants with atypia on ductal lavage in which ductal lesions can be identified using ductoscopy.

OUTLINE: This is a multicenter study.

Participants undergo nipple aspirate fluid and ductal lavage fluid collection. Fluid samples are analyzed using cytological analysis, immunohistochemistry, fluorescent in situ hybridization, or in situ polymerase chain reaction. Protein patterns are identified using mass spectrometry.

Participants found to have atypia undergo repeat lavage at 3-6 months and 1 year. Participants with normal fluid samples undergo repeat lavage at 1 year.

PROJECTED ACCRUAL: A total of 30 participants will accrued for this study within 2-3 months.

Conditions

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Breast Cancer

Keywords

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breast cancer

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Interventions

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polymerase chain reaction

Intervention Type GENETIC

proteomic profiling

Intervention Type GENETIC

cytology specimen collection procedure

Intervention Type OTHER

immunohistochemistry staining method

Intervention Type OTHER

breast duct lavage

Intervention Type PROCEDURE

fluorescence in situ hybridization

Intervention Type GENETIC

Eligibility Criteria

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Inclusion Criteria

DISEASE CHARACTERISTICS:

* High risk for breast cancer defined as 1 of the following:

* History of atypical hyperplasia or atypia found on biopsy, fine-needle aspiration, or ductal lavage
* Family history of cancer meeting at least 1 of the following criteria:

* Single relative with multiple primary cancers
* One or more relative under 40 years of age with breast cancer OR bilateral breast cancer
* Two or more relatives with ovarian cancer
* Two or more relatives with breast cancer and 1 is under 50 years of age
* One or more relative with breast cancer plus ≥ 1 relative with ovarian cancer
* Ashkenazi Jewish descent AND relative with breast cancer under 50 years of age OR ovarian cancer at any age
* BRCA1 and/or BRCA2 mutation
* Prior breast cancer in contralateral breast
* Gail risk \> 1.7%
* Spontaneous nipple discharge
* Serum estradiol \> 10 pmol/L
* Hormone receptor status:

* Not specified

PATIENT CHARACTERISTICS:

Age

* Over 18

Sex

* Female

Menopausal status

* Not specified

Performance status

* Not specified

Life expectancy

* Not specified

Hematopoietic

* Not specified

Hepatic

* Not specified

Renal

* Not specified

Other

* Able to obtain breast duct fluids

PRIOR CONCURRENT THERAPY:

Biologic therapy

* Not specified

Chemotherapy

* Not specified

Endocrine therapy

* More than 4 months since prior tamoxifen or raloxifene

Radiotherapy

* No prior radiotherapy to the breast

Surgery

* No prior incisional or excisional biopsy within 1.5 cm of nipple

Other

* No prior neoadjuvant therapy
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

University of California, San Francisco

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Laura J. Esserman, MD, MBA

Role: PRINCIPAL_INVESTIGATOR

University of California, San Francisco

Locations

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UCSF Comprehensive Cancer Center

San Francisco, California, United States

Site Status

Countries

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United States

Other Identifiers

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UCSF-TEMP01

Identifier Type: -

Identifier Source: secondary_id

CDR0000378088

Identifier Type: -

Identifier Source: org_study_id