Trial Outcomes & Findings for Vaccine Therapy For Patients Being Considered For Organ Transplant Who Are at Risk For PTLD (NCT NCT00278200)

NCT ID: NCT00278200

Last Updated: 2023-04-25

Results Overview

Percentage of participants with T-cell responses. For participants who were EBV-seronegative at enrollment, a response is defined as the appearance of EBV-specific T-cells at one month after the second injection. For participants who were EBV-seropositive at enrollment, a response is defined as a two-fold increase over baseline in the frequency of CD8+ T-cells responding to EBV latency antigens at any point during the first 67 days following the first injection.

Recruitment status

TERMINATED

Study phase

PHASE1

Target enrollment

23 participants

Primary outcome timeframe

Up to 67 days

Results posted on

2023-04-25

Participant Flow

One subject on the EBV seronegative arm was a screen failure. One EBV seronegative subject was not assigned to intervention arm due to physician decision. Seven EBV seropositive subjects were not assigned to intervention arm: three due to physician decision; one due to loss of follow-up; one due to death; and two due to subject withdrawal.

Participant milestones

Participant milestones
Measure
EBV Seronegative
Inactivated EBV-infected vaccine given at Week 0 and Week 4. This arm included all participants who were negative for Epstein-Barr Virus (EBV) at baseline.
EBV Seropositive
Inactivated EBV-infected vaccine given at Week 0 and Week 4. This arm included all participants who were positive for Epstein-Barr Virus (EBV) at baseline.
Overall Study
STARTED
2
12
Overall Study
COMPLETED
1
8
Overall Study
NOT COMPLETED
1
4

Reasons for withdrawal

Reasons for withdrawal
Measure
EBV Seronegative
Inactivated EBV-infected vaccine given at Week 0 and Week 4. This arm included all participants who were negative for Epstein-Barr Virus (EBV) at baseline.
EBV Seropositive
Inactivated EBV-infected vaccine given at Week 0 and Week 4. This arm included all participants who were positive for Epstein-Barr Virus (EBV) at baseline.
Overall Study
Death
1
3
Overall Study
Physician Decision
0
1

Baseline Characteristics

Race and Ethnicity were not collected from any participant.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
EBV Seronegative
n=2 Participants
Inactivated EBV-infected vaccine given at Week 0 and Week 4. This arm included all participants who were negative for Epstein-Barr Virus (EBV) at baseline. Inactivated EBV-infected vaccine
EBV Seropositive
n=12 Participants
Inactivated EBV-infected vaccine given at Week 0 and Week 4. This arm included all participants who were positive for Epstein-Barr Virus (EBV) at baseline. Inactivated EBV-infected vaccine
Total
n=14 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=2 Participants
2 Participants
n=12 Participants
2 Participants
n=14 Participants
Age, Categorical
Between 18 and 65 years
2 Participants
n=2 Participants
10 Participants
n=12 Participants
12 Participants
n=14 Participants
Age, Categorical
>=65 years
0 Participants
n=2 Participants
0 Participants
n=12 Participants
0 Participants
n=14 Participants
Age, Continuous
38 years
n=2 Participants
54 years
n=12 Participants
54 years
n=14 Participants
Sex: Female, Male
Female
0 Participants
n=2 Participants
8 Participants
n=12 Participants
8 Participants
n=14 Participants
Sex: Female, Male
Male
2 Participants
n=2 Participants
4 Participants
n=12 Participants
6 Participants
n=14 Participants
Race and Ethnicity Not Collected
0 Participants
Race and Ethnicity were not collected from any participant.
Region of Enrollment
United States
2 Participants
n=2 Participants
12 Participants
n=12 Participants
14 Participants
n=14 Participants

PRIMARY outcome

Timeframe: Up to 67 days

Population: T-cell responses were uninterpretable on lab analysis; therefore, data was not collected to assess this outcome measure.

Percentage of participants with T-cell responses. For participants who were EBV-seronegative at enrollment, a response is defined as the appearance of EBV-specific T-cells at one month after the second injection. For participants who were EBV-seropositive at enrollment, a response is defined as a two-fold increase over baseline in the frequency of CD8+ T-cells responding to EBV latency antigens at any point during the first 67 days following the first injection.

Outcome measures

Outcome data not reported

SECONDARY outcome

Timeframe: Up to 5 years

Number of participants who received at least one vaccination and experienced at least one grade 3-4 adverse event by CTCAE 2.0 that was attributed to protocol therapy.

Outcome measures

Outcome measures
Measure
EBV Seronegative
n=2 Participants
Inactivated EBV-infected vaccine given at Week 0 and Week 4. This arm included all participants who were negative for Epstein-Barr Virus (EBV) at baseline. Inactivated EBV-infected vaccine
EBV Seropositive
n=12 Participants
Inactivated EBV-infected vaccine given at Week 0 and Week 4. This arm included all participants who were positive for Epstein-Barr Virus (EBV) at baseline. Inactivated EBV-infected vaccine
Adverse Events Associated With the Vaccine
0 Participants
0 Participants

SECONDARY outcome

Timeframe: Up to 5 years

Population: Only one participant met the criteria described in the outcome description. This participant was never tested post-transplant for EBV, so no data was collected for this outcome measure.

Number of participants who were EBV-seronegative at baseline, received at least one vaccination, subsequently received a solid organ transplant (not part of this protocol), and did not develop a primary EBV infection.

Outcome measures

Outcome data not reported

Adverse Events

EBV Seronegative

Serious events: 1 serious events
Other events: 1 other events
Deaths: 1 deaths

EBV Seropositive

Serious events: 2 serious events
Other events: 9 other events
Deaths: 4 deaths

Serious adverse events

Serious adverse events
Measure
EBV Seronegative
n=2 participants at risk
Inactivated EBV-infected vaccine given at Week 0 and Week 4. This arm included all participants who were negative for Epstein-Barr Virus (EBV) at baseline.
EBV Seropositive
n=12 participants at risk
Inactivated EBV-infected vaccine given at Week 0 and Week 4. This arm included all participants who were positive for Epstein-Barr Virus (EBV) at baseline.
Infections and infestations
Bacteremia
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
Infections and infestations
Peritonitis
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
Respiratory, thoracic and mediastinal disorders
Acute lung injury
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
Respiratory, thoracic and mediastinal disorders
Respiratory distress
50.0%
1/2 • Number of events 1 • Up to 5 years
0.00%
0/12 • Up to 5 years

Other adverse events

Other adverse events
Measure
EBV Seronegative
n=2 participants at risk
Inactivated EBV-infected vaccine given at Week 0 and Week 4. This arm included all participants who were negative for Epstein-Barr Virus (EBV) at baseline.
EBV Seropositive
n=12 participants at risk
Inactivated EBV-infected vaccine given at Week 0 and Week 4. This arm included all participants who were positive for Epstein-Barr Virus (EBV) at baseline.
Hepatobiliary disorders
Ascites
0.00%
0/2 • Up to 5 years
16.7%
2/12 • Number of events 2 • Up to 5 years
Skin and subcutaneous tissue disorders
Bruising
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 2 • Up to 5 years
General disorders
Cough
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
Nervous system disorders
Diplopia
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
General disorders
Headache
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 2 • Up to 5 years
General disorders
Hypertension
0.00%
0/2 • Up to 5 years
16.7%
2/12 • Number of events 2 • Up to 5 years
Skin and subcutaneous tissue disorders
Injection site reaction
50.0%
1/2 • Number of events 4 • Up to 5 years
41.7%
5/12 • Number of events 15 • Up to 5 years
Investigations
Leukopenia
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
Gastrointestinal disorders
Nausea
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
General disorders
Pain - unspecified
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
Gastrointestinal disorders
Pain - abdomen
50.0%
1/2 • Number of events 1 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
Skin and subcutaneous tissue disorders
Pain - axilla
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 2 • Up to 5 years
Respiratory, thoracic and mediastinal disorders
Runny nose
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
Gastrointestinal disorders
Sore throat
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
Infections and infestations
Streptococcal pharyngitis
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
Investigations
Thrombocytopenia
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years
Gastrointestinal disorders
Umbilical hernia
0.00%
0/2 • Up to 5 years
16.7%
2/12 • Number of events 2 • Up to 5 years
Gastrointestinal disorders
Vomiting
0.00%
0/2 • Up to 5 years
8.3%
1/12 • Number of events 1 • Up to 5 years

Additional Information

Richard Ambinder, MD, PhD

Johns Hopkins University

Phone: 4109558839

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place