ctDNA Positive Patients Diagnosed With cT1bN0 and cT1cN0 TNBC

NCT ID: NCT07332975

Last Updated: 2026-01-12

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Total Enrollment

77 participants

Study Classification

OBSERVATIONAL

Study Start Date

2026-01-31

Study Completion Date

2029-01-31

Brief Summary

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This translational biomarker study will collect blood in patients Stage I triple negative breast cancer at diagnosis prior to either neoadjuvant treatment or surgery and then during treatment.

Detailed Description

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A highly sensitive, tumor-informed method of detecting circulating free DNA (cfDNA) shed by solid tumors has been developed. Plasma will be assayed for ctDNA using the SaferSeqS tumor-informed assay employing DNA sequences derived from breast biopsies and/ or surgery specimens. The abundance and molecular characteristics of ctDNA will be evaluated in 77 patients using optimum Simon Stage II statistical design.

Conditions

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Triple Negative Breast Cancer

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Eligibility Criteria

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Inclusion Criteria

1. Patients must be ≥18 years of age
2. Cytologically or histologically confirmed and previously untreated invasive breast cancer that is:

* Clinical stage I
* ≤2 cm on imaging
* cN0
* cM0
* Grade 2 or Grade 3
3. ECOG 0-2
4. Locally assessed ER+ in 0% of cells.
5. Locally assessed HER2-negative (IHC 2+/FISH-negative or IHC 0 or 1+ /either FISH-negative or untested).
6. No prior chemotherapy
7. Fit for chemotherapy and surgery
8. Must have the ability to understand and the willingness to sign a written informed consent document.
9. Must be willing to provide serial blood samples for the study.

Exclusion Criteria

1. History of other invasive malignancy in the past 2 years
2. Synchronous breast cancers and multifocal/multicentric breast cancers
3. Medical condition or social situation that may preclude adherence to the protocol.
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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University of Pittsburgh

OTHER

Sponsor Role lead

Responsible Party

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Marija Balic, MD PhD

Professor of Medicine, Hematology Oncology

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Marija Balic, MD

Role: PRINCIPAL_INVESTIGATOR

UPMC Hillman Cancer Center

Locations

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UPMC Hillman Cancer

Pittsburgh, Pennsylvania, United States

Site Status

Countries

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United States

Central Contacts

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Kelsey Mitch, BSN

Role: CONTACT

4126412357

Lucia M Borrasso, BSN

Role: CONTACT

4126413304

Facility Contacts

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Kelsey Mitch, BSN

Role: primary

4126412357

Lucia M Borrasso, BSN

Role: backup

412-641-3304

Other Identifiers

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HCC 25-105

Identifier Type: -

Identifier Source: org_study_id

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