Cellular-Level Fluorescence Imaging for Intraoperative Margin Assessment in Breast Cancer

NCT ID: NCT07311317

Last Updated: 2025-12-30

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Total Enrollment

165 participants

Study Classification

OBSERVATIONAL

Study Start Date

2026-01-31

Study Completion Date

2027-01-31

Brief Summary

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Accurate intraoperative margin assessment in breast cancer surgery remains challenging. Current reliance on frozen section pathology is limited by prolonged processing time, sampling constraints, and resource intensity, contributing to variable outcomes.

The EndoSCell Scanner is a novel cellular-resolution fluorescence-guided imaging technology designed to address these limitations. It enables real-time, pathology-level visualization of malignant cellular features at the surgical bed. With a 3mm probe allowing comprehensive 360° margin assessment, it reduces procedural time to under five minutes and may significantly lower secondary surgery rates.

This prospective, self-controlled study aims to evaluate the accuracy of the EndoSCell Scanner for intraoperative margin assessment in patients undergoing mastectomy. Its performance will be systematically compared against both frozen section and final paraffin pathology to validate sensitivity and operational efficiency. If proven effective, this technology could become a standard adjunct tool, standardizing precision in breast cancer surgery and improving patient outcomes by enhancing surgical consistency.

Detailed Description

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This study is a multicenter, prospective, self-controlled study. Basis for Sample Size Calculation: According to the results of the first phase, the accuracy of frozen pathology is 98.2%. Assuming that the detection interpretation results of ES are not inferior to intraoperative frozen pathology, a one-sided test will be conducted, with α=0.02, 1-β=0.8, and the non-inferiority margin is 0.02. The estimated required sample size is 593. Considering a 10% exclusion rate, the total sample size will be 659. Each patient is expected to have at least 4 samples taken, totaling 165 patients.

Data Collection:1)Basic patient information: age, gender; 2)Tumor diagnosis (including imaging diagnosis): location, size, and staging; 3)Intraoperative collected information: surgery start time, surgery end time; 4)Training and testing records of the physicians in the ES interpretation group; 5)Interpretation records: a.ES start time, ES interpretation end time, ES interpretation results, ES image satisfaction; b.Frozen pathology submission time, report receipt time, report results; c.Paraffin pathology submission time, report time, and report results.

The statistical analysis will be performed using SAS statistical software, version 9.4 or higher. All statistical tests will be two-sided. A P value less than or equal to 0.05 will be considered statistically significant. The description of quantitative indicators will include the mean, standard deviation, median, minimum, maximum, and interquartile range. The description of categorical indicators will use the number and percentage of each category. The comparison of general conditions between the two groups will be analyzed using appropriate methods based on the type of indicators. The consistency evaluation of quantitative data will use the Bland-Altman method and ICC.

Conditions

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Breast Cancer Early Stage Breast Cancer (Stage 1-3)

Keywords

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Breast Cancer, Fluorescence-guided Cellular

Study Design

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Observational Model Type

OTHER

Study Time Perspective

PROSPECTIVE

Eligibility Criteria

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Inclusion Criteria

* Age between 18 - 70 years
* Patients scheduled for breast tumor resection
* Clinically or radiologically showing a solitary lesion and

1. (Before neoadjuvant chemotherapy) Maximum tumor diameter ≤ 5cm;
2. cN0-1;
3. No clinical or imaging evidence of nipple, skin, chest wall invasion, or distant metastasis before surgery.
* Subjects voluntarily sign informed consent or are exempted from signing upon approval by the ethics committee.

Exclusion Criteria

* Inflammatory breast cancer;
* Patients allergic to methylene blue and sodium fluorescein;
* Unable to understand the trial requirements or unable to complete the study follow-up plan;
* Pregnant and breastfeeding women;
* Patients unable to complete follow-up due to mental illness, cognitive or emotional disorders;
* Those with severe comorbidities (such as organ dysfunction, immune deficiency) or poor general condition;
* Those participating in other clinical studies that may affect this trial;
* Those for whom satisfactory specimens for both frozen pathology and paraffin pathology cannot be obtained during surgery;
* Those who cannot obtain a paraffin pathology report.
Minimum Eligible Age

18 Years

Maximum Eligible Age

70 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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West China Fourth Hospital, Sichuan University

UNKNOWN

Sponsor Role collaborator

The Fourth People's Hospital of Sichuan Province

UNKNOWN

Sponsor Role collaborator

West China Hospital

OTHER

Sponsor Role lead

Responsible Party

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Du Zhenggui

deputy director of breast center

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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the Fourth People'S Hospital of Sichuan Province

Chengdu, , China

Site Status

West China Fourth Hospital, Sichuan University

Chengdu, , China

Site Status

West China Hospital

Chengdu, , China

Site Status

Countries

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China

Central Contacts

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Zhenggui DU

Role: CONTACT

Phone: +86 13880768222

Email: [email protected]

Facility Contacts

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donglin zhang

Role: primary

bin ma

Role: primary

zhenggui Du

Role: primary

Other Identifiers

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2025-1955

Identifier Type: -

Identifier Source: org_study_id