Combination of Bitter Melon Extract and Snakehead Fish Powder to Reduce Glycaemia in Type 2 Diabetes Mellitus

NCT ID: NCT07303127

Last Updated: 2025-12-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

80 participants

Study Classification

INTERVENTIONAL

Study Start Date

2021-06-01

Study Completion Date

2021-10-01

Brief Summary

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Type 2 Diabetes Mellitus (T2DM) is a chronic metabolic disorder characterized by persistent hyperglycemia, which contributes to the formation of advanced glycation end-products (AGEs) and increases the risk of long-term complications. Bitter melon (Momordica charantia L.) contains bioactive compounds that may support glycemic control, while snakehead fish (Channa striata) provides amino acids and fatty acids with potential antioxidant and restorative properties.

This study aims to evaluate the effect of a combined supplementation of bitter melon extract and snakehead fish powder on glycemic parameters in adults with T2DM. The study uses a quasi-experimental design with a control group, involving 80 participants recruited from community health centers in Surakarta, Indonesia. Participants are allocated to either a treatment group receiving the herbal combination or a control group receiving a placebo for four weeks.

Glycemic and biochemical parameters-including fasting plasma glucose, glycated albumin, insulin levels, and AGEs-are measured at baseline and at the end of the intervention period. Safety monitoring and adherence assessment are conducted throughout the study. This pilot investigation aims to explore the potential role of the combined supplement in the management of T2DM.

Detailed Description

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Type 2 Diabetes Mellitus (T2DM) is a chronic metabolic condition marked by persistent hyperglycemia and associated inflammation, which contribute to the development of vascular and metabolic complications. Chronic hyperglycemia accelerates the formation of advanced glycation end-products (AGEs), which may impair tissue structure and function through oxidative stress and protein cross-linking mechanisms. Dietary phytochemicals and bioactive compounds have been increasingly studied as supportive strategies for glycemic regulation.

Bitter melon (Momordica charantia L.) has been traditionally used in various cultures as a natural remedy for diabetes. It contains several bioactive molecules, including alkaloids, flavonoids, catechins, saponins, charantins, and polypeptide-p, which are believed to contribute to glycemic regulation through mechanisms such as insulin secretagogue effects, enhancement of glucose utilization, modulation of glucose transporters, and inhibition of α-glucosidase activity. Despite its widespread traditional use, clinical findings have been variable due to differences in formulations, study durations, and methodological designs.

Snakehead fish (Channa striata) contains essential amino acids, fatty acids, and antioxidant components that may support metabolic processes and physiological recovery. While research has explored its nutritional and antioxidant potential, its combined use with bitter melon in T2DM has not been previously studied.

This study adopts a quasi-experimental design with a control group to assess the effect of a combined herbal supplement consisting of bitter melon extract and snakehead fish powder in individuals with T2DM. A total of 80 participants aged 25-65 years are recruited from four community health centers in Surakarta, Indonesia. Eligible participants have been clinically diagnosed with T2DM, are receiving metformin therapy, and meet specified biochemical inclusion criteria. Individuals with impaired renal or hepatic function, pregnancy, lactation, or known allergies to the study materials are excluded.

Participants provide informed consent and undergo initial screening before being allocated to either the treatment group or the placebo group. The intervention is administered for a period of four weeks. Outcome assessments include fasting plasma glucose, glycated albumin, insulin, and AGEs, measured at baseline and at the end of week four. Laboratory analyses utilize enzymatic methods and ELISA techniques. Medication adherence is monitored through pill counts and structured interviews during follow-up visits.

Throughout the study, participants are monitored for safety, tolerability, and any potential adverse events. This pilot study is intended to generate preliminary evidence on the feasibility and potential physiological effects of combining bitter melon extract and snakehead fish powder in the management of T2DM, and to inform the design of larger future clinical trials.

Conditions

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Type 2 Diabetes Mellitus (T2DM)

Keywords

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AGEs Bitter melon Channa striata Type 2 Diabetes Mellitus Hypoglycaemic effect

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

A quasi-experimental parallel-group study in which participants are assigned to either the herbal supplement group or the placebo control group without randomization
Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Not applicable. This study did not implement masking; both investigators and participants were aware of group assignments

Study Groups

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Intervention group

Participants in this arm receive a combination supplement containing bitter melon (Momordica charantia L.) extract and snakehead fish (Channa striata) powder. The supplement is administered in capsule form for 4 weeks in addition to their routine metformin therapy.

Group Type EXPERIMENTAL

Bitter Melon Extract and Snakehead Fish Powder Combination

Intervention Type DIETARY_SUPPLEMENT

Participants in the intervention group receive a combined herbal supplement consisting of bitter melon (Momordica charantia L.) extract and snakehead fish (Channa striata) powder. The supplement is prepared in capsule form at the Clinical Pharmacy Laboratory of Universitas Muhammadiyah Surakarta. Participants take the supplement daily for 4 weeks while continuing their routine metformin therapy. The formulation is standardized by maceration of bitter melon with ethanol followed by evaporation, and preparation of snakehead fish powder through steaming, drying, and milling before encapsulation.

Control group

Participants in this arm receive placebo capsules designed to match the appearance of the herbal supplement. The placebo is administered for 4 weeks while participants continue their routine metformin therapy.

Group Type PLACEBO_COMPARATOR

Placebo Capsule

Intervention Type OTHER

Participants in the control group receive placebo capsules that are visually identical to the herbal supplement capsules. The placebo contains inert, non-active ingredients and is administered daily for 4 weeks. The placebo is designed to match the appearance and dosing schedule of the active supplement but contains no bitter melon extract or snakehead fish powder. Participants continue their routine metformin therapy during the study.

Interventions

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Bitter Melon Extract and Snakehead Fish Powder Combination

Participants in the intervention group receive a combined herbal supplement consisting of bitter melon (Momordica charantia L.) extract and snakehead fish (Channa striata) powder. The supplement is prepared in capsule form at the Clinical Pharmacy Laboratory of Universitas Muhammadiyah Surakarta. Participants take the supplement daily for 4 weeks while continuing their routine metformin therapy. The formulation is standardized by maceration of bitter melon with ethanol followed by evaporation, and preparation of snakehead fish powder through steaming, drying, and milling before encapsulation.

Intervention Type DIETARY_SUPPLEMENT

Placebo Capsule

Participants in the control group receive placebo capsules that are visually identical to the herbal supplement capsules. The placebo contains inert, non-active ingredients and is administered daily for 4 weeks. The placebo is designed to match the appearance and dosing schedule of the active supplement but contains no bitter melon extract or snakehead fish powder. Participants continue their routine metformin therapy during the study.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Diagnosed with Type 2 Diabetes Mellitus (T2DM).
* Age 25-65 years.
* Currently treated with one type of biguanide (metformin).
* Fasting plasma glucose 126-200 mg/dL.
* Willing to participate and sign informed consent.
* Able to attend all study visits at participating health centers.

Exclusion Criteria

* Positive pregnancy test or currently breastfeeding.
* Impaired renal function (creatinine \> 1.5 mg/dL).
* Impaired liver function (SGOT \> 40 U/L or SGPT \> 35 U/L, or ≥2× upper normal limit).
* Known allergy to bitter melon extract or snakehead fish powder.
* Any condition judged by investigators to interfere with study participation.
* Participants who withdraw consent or are unable to complete the intervention.
Minimum Eligible Age

25 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Universitas Muhammadiyah Surakarta

OTHER

Sponsor Role lead

Responsible Party

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Fahrun Nur Rosyid

Head of Quality Assurance Group

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Faculty of Health Sciences FIK UMS

Role: PRINCIPAL_INVESTIGATOR

Universitas Muhammadiyah Surakarta

Locations

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Faculty of Health Sciences, Universitas Muhammadiyah Surakarta

Surakarta, Centre Java, Indonesia

Site Status

Countries

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Indonesia

Other Identifiers

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UMS-MOMORDICA-RCT2025

Identifier Type: -

Identifier Source: org_study_id