The Effect of Hydration Status on Substrate Oxidation at Rest and During Light and Moderate Exercise
NCT ID: NCT07298863
Last Updated: 2025-12-23
Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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RECRUITING
NA
74 participants
INTERVENTIONAL
2025-11-05
2026-04-30
Brief Summary
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While there is some evidence that low fluid intake may lead to impaired metabolic flexibility, more information is needed. Therefore, the purpose of this study is to evaluate the impact of hydration status on substrate preference at rest and during exercise at varying intensities (light and moderate). Further, we will examine whether biological sex and menstrual cycle phase impact hydration and metabolism under these same conditions.
Detailed Description
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Conditions
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Keywords
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Study Design
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RANDOMIZED
CROSSOVER
OTHER
NONE
Study Groups
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Euhydrated with electrolyte beverage
Euhydrated with electrolyte beverage (220 mg sodium in 20 oz) to account for sweat loss and to maintain hydration over 24-hour period
Exercise Trial
Perform steady-state cycling exercise for approximately 1 hour at 70-80% of maximal heart rate
Experimental Trial
24h after exercise trial, following assigned 24h hydration plan, perform indirect calorimetry followed by 30 min of light cycling (\~50% VO2 max) then 30 min of moderate-high cycling (\~80% VO2 max).
Euhydrated with water
Euhydrated with water to account for sweat loss and to maintain hydration over 24-hour period
Exercise Trial
Perform steady-state cycling exercise for approximately 1 hour at 70-80% of maximal heart rate
Experimental Trial
24h after exercise trial, following assigned 24h hydration plan, perform indirect calorimetry followed by 30 min of light cycling (\~50% VO2 max) then 30 min of moderate-high cycling (\~80% VO2 max).
Dehydrated control trial
Fluid restriction during exercise and for remaining 24 hours
Exercise Trial
Perform steady-state cycling exercise for approximately 1 hour at 70-80% of maximal heart rate
Experimental Trial
24h after exercise trial, following assigned 24h hydration plan, perform indirect calorimetry followed by 30 min of light cycling (\~50% VO2 max) then 30 min of moderate-high cycling (\~80% VO2 max).
Interventions
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Exercise Trial
Perform steady-state cycling exercise for approximately 1 hour at 70-80% of maximal heart rate
Experimental Trial
24h after exercise trial, following assigned 24h hydration plan, perform indirect calorimetry followed by 30 min of light cycling (\~50% VO2 max) then 30 min of moderate-high cycling (\~80% VO2 max).
Eligibility Criteria
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Inclusion Criteria
* If female, subject is not pregnant (based on self-report)
* If female, subject has stopped taking birth control medication or removed long-acting reversible contraceptive \>6 months at start of the study
* If female, subject has normal menstrual cycle (21-35 days)
* If female, subject is willing to track their menstrual cycle
* If female, subject has a smartphone compatible for application to track menstrual cycle (female only) and/or wearable data (females and males)
* Subject is 18-35 years of age, inclusive
* Subject body mass index (BMI) is between 18.5-25 kg/m2
* Subject is at least recreationally active (engaged in moderate-intensity, intermittent, or steady-state exercise at least 3 days per week for at least 60 min at a time)
* Subject is accustomed to cycling for at least 60 min
* Subject does not smoke (or has quit for at least 6 months)
* Subject is not taking medication that may interfere with the study (e.g., diuretics, laxatives, or weight loss drugs)
* Subject has no health conditions that would interfere with the study as indicated on the general health questionnaire (GHQ) e.g. cardiovascular, renal, or metabolic diseases
* Subject is not actively involved in weight loss intervention (dieting and/or anti-obesity medications)
* Subject is not allergic to adhesives (e.g., medical tape)
* Subject is willing to avoid alcohol consumption 24 hours prior to visit(s)
* Subject is willing to fast overnight (\~8-12 hours)
* Subject is willing to refrain from vigorous exercise for 24 hours (light physical activity only)
* Subject is willing to be shaved at patch or electrode site, if necessary
* Subject is willing to eat the exact same food the day prior to each visit to the laboratory
* Able to speak, write, and read English
* Provision of written consent to participate
Exclusion Criteria
* Subject has participated in any PepsiCo trial within past 36 months
* Subject has a condition or is taking medication that the investigator believes would interfere with his/her ability to provide informed consent, comply with the study protocol, which might confound the interpretation of the study results, or put the person at undue risk
* Subject is allergic to alcohol (alcohol pre pads are used for blood draws
* If female, subject is taking birth control medication or has long-acting reversible contraceptive
* If male, subject is undergoing hormone therapy to alter testosterone levels (injections, transdermal patch, pill, etc.)
* Subject is employed by, or has a parent, guardian, or other immediate family member employed by a company that manufactures any products that compete with any Gatorade product. If subject is unsure if a company would be considered a competitor to Gatorade, they will be asked to please let the study investigator know the name of the other company and the nature of their relationship to that company before they sign the informed consent.
18 Years
35 Years
ALL
Yes
Sponsors
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University of Ontario Institute of Technology
OTHER
PepsiCo Global R&D
INDUSTRY
Responsible Party
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Locations
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Gatorade Sports Science Institute
Valhalla, New York, United States
University Of Ontario Institute Of Technology
Oshawa, Ontario, Canada
Countries
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Central Contacts
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Facility Contacts
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Matt Hinkley
Role: primary
Heather Sprenger
Role: primary
Other Identifiers
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PEP-2507
Identifier Type: -
Identifier Source: org_study_id