Trial of Aldosterone-hybrid SteRoid for Guiding curablE Treatment of Primary Aldosteronism

NCT ID: NCT07297745

Last Updated: 2025-12-22

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Clinical Phase

NA

Total Enrollment

120 participants

Study Classification

INTERVENTIONAL

Study Start Date

2026-01-02

Study Completion Date

2029-12-31

Brief Summary

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The goal of this project is to improve the diagnostic pathway for patients with primary aldosteronism, by validating non-invasive biomarkers coupled with routine computed tomography imaging. The aim is to reduce reliance on invasive procedures, shorten diagnostic timelines, and enhance accessibility to definitive diagnosis and treatment globally.

Detailed Description

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Primary Aldosteronism is a common but under-diagnosed condition, despite being a curable cause of secondary hypertension with significant long-term health consequences if left untreated.

Once the diagnosis of primary aldosteronism is confirmed, subtype tests are required to demonstrate unilateral primary aldosteronism before curative surgery. Most patients need to undergo adrenal vein sampling, an invasive procedure performed under radiological guidance. However, this procedure is technically-challenging, with failure to cannulate both adrenal veins frequently leading to inconclusive results.

Many patients worldwide may be offered surgery based on computed tomography imaging alone because of a lack of adrenal vein sampling expertise or a failed adrenal vein sampling result. High hybrid hormones indicate a functional KCNJ5-mutant aldosterone-producing adenoma and coupled with computed tomography imaging may provide a non-invasive alternative to adrenal vein sampling for identifying unilateral primary aldosteronism.

Ultimately, this trial aims to reduce the number of patients that will require an invasive adrenal vein sampling in future clinical diagnostics. This will improve access, and allow more patients to receive timely and curative treatment.

We will have dual primary outcomes:

1. To determine the sensitivity of hybrid hormones combined with computed tomography imaging in identifying unilateral primary aldosteronism.
2. To determine the specificity of hybrid hormones combined with computed tomography imaging in identifying KCNJ5-mutant aldosterone-producing adenomas.

Conditions

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Hyperaldosteronism; Primary Primary Aldosteronism Due to Aldosterone Producing Adenoma

Keywords

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Hybrid hormone Subtyping adrenalectomy adrenal vein sampling biomarkers steroid profiling

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

We will prospectively recruit patients with primary aldosteronism who are keen for surgery if unilateral disease is diagnosed. All participants recruited will undergo both routine subtyping (adrenal vein sampling and/or molecular imaging) and research subtyping (blood tests for hybrid hormone coupled with computed tomography).

All results will be reviewed at a multi-disciplinary meeting, with computed tomography discussed first (radiologist will be masked to results of hybrid hormones and adrenal vein sampling), then hybrid hormones, then adrenal vein sampling. Patients with either adrenal vein sampling lateralization, or elevated hybrid hormones with a unilateral adrenal nodule and normal contralateral gland on computed tomography will be offered surgery. All patients will be re-evaluated post-surgery for biochemical cure of primary aldosteronism (co-primary outcome). Genetic sequencing will be performed on resected adrenals for KCNJ5 mutation (co-primary outcome).
Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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Hybrid hormones

Previous studies have shown that hybrid hormones are elevated in patients with unilateral primary aldosteronism who have large, visible adenomas in their adrenal glands. These tumors harbour a somatic KCNJ5 mutation and are also most likely to be cured of hypertension after surgery. Hence, we hypothesize that high hybrid hormones levels, in the presence of an adenoma on a computed tomography imaging would identify patients who can proceed to surgery.

Group Type EXPERIMENTAL

Hybrid hormones

Intervention Type DIAGNOSTIC_TEST

Participants will have a baseline blood sample taken so we can measure the adrenal steroid biomarkers 18-oxocortisol and 18-hydroxylcortisol and other steroids. The collected plasma or serum will then be analyzed in the laboratory using validated methods, such as liquid chromatography-tandem mass spectrometry (LC-MS/MS), to determine the levels of these biomarkers.

Interventions

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Hybrid hormones

Participants will have a baseline blood sample taken so we can measure the adrenal steroid biomarkers 18-oxocortisol and 18-hydroxylcortisol and other steroids. The collected plasma or serum will then be analyzed in the laboratory using validated methods, such as liquid chromatography-tandem mass spectrometry (LC-MS/MS), to determine the levels of these biomarkers.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Patients with diagnosis of primary aldosteronism according to Endocrine Society guidelines 2025.
* Willing to undergo subtype testing with adrenal vein sampling and/or molecular imaging (Positron Emission Tomography/Computed Tomography, PET/CT)
* Keen for surgical treatment if shown to have unilateral primary aldosteronism.

Exclusion Criteria

* Inability to provide written informed consent
Minimum Eligible Age

21 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Singapore General Hospital

OTHER

Sponsor Role collaborator

Sengkang General Hospital

OTHER

Sponsor Role collaborator

National University Hospital, Singapore

OTHER

Sponsor Role collaborator

Changi General Hospital

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Troy Puar, FRCP, PhD

Role: PRINCIPAL_INVESTIGATOR

Changi General Hospital

Locations

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Changi General Hospital

Singapore, Singapore, Singapore

Site Status

National University Hospital

Singapore, , Singapore

Site Status

Sengkang General Hospital

Singapore, , Singapore

Site Status

Singapore General Hospital

Singapore, , Singapore

Site Status

Countries

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Singapore

Central Contacts

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Troy H Puar, FRCP, PhD

Role: CONTACT

Phone: 67888833

Email: [email protected]

Facility Contacts

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Troy H Puar, FRCP, PhD

Role: primary

Ada Teo

Role: primary

Matthew Chuah

Role: primary

Swee Du Soon

Role: primary

Other Identifiers

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TARGET-PA

Identifier Type: -

Identifier Source: org_study_id