Antibody-based PET Imaging and Treatment Response in Breast Cancer Treated With an Antibody-drug Conjugate.

NCT ID: NCT07294430

Last Updated: 2025-12-19

Basic Information

• Recruitment Status: NOT_YET_RECRUITING
• Clinical Phase: PHASE2
• Total Enrollment: 30 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2025-12-31
• Study Completion Date: 2030-12-31

Brief Summary

OASIS-ImmunoPET is a monocentric pilot study evaluating antibody imaging to predict response to antibody-drug conjugate (ADC), an innovative cancer targeted therapy, and potentially replace tumor biopsy. It is addressed to patients with locally advanced or metastatic breast cancer who are eligible to receive the ADC Trastuzumab deruxtecan (T-DXd) according to local approval, and who are already enrolled in OASIS study (NCT pending).

Detailed Description

Antibody-drug conjugates (ADCs) have emerged as a transformative class of cancer targeted therapies, combining the specificity of monoclonal antibodies with the potency of cytotoxic payloads. This dual mechanism allows ADCs to selectively target tumor cells while minimizing systemic toxicity, thereby offering a more refined approach compared to conventional chemotherapy.

ADCs are made of 3 components: a monoclonal antibody specifically targeting tumor cells, a highly potent cytotoxic payload, and a special linker that connects the antibody to the drug. Their multi-step mode of action implies that ADC activity relies on multiple factors, such as target distribution, target density, internalization capability, linker cleavage, payload sensitivity and tumor-microenvironment modulation.

Over the past decade, ADCs have demonstrated significant improvements in survival for patients with various solid tumors and hematological malignancies. Despite these advancements, resistance to ADCs remains a major clinical challenge. Most patients who initially respond to ADC therapy eventually develop resistance, leading to disease progression. The mechanisms underlying ADC resistance are complex and poorly understood, involving tumor heterogeneity, drug metabolism, immune evasion, and alterations in target antigen expression.

Currently, no validated predictive biomarkers exist to guide ADC selection, treatment sequencing, or resistance monitoring. As a result, clinicians lack reliable tools to personalize ADC therapy, limiting the ability to optimize patient outcomes. A deeper understanding of ADC response and resistance mechanisms is urgently needed to define and adopt optimal companion diagnostics for currently approved and forthcoming ADCs and to obtain decision support tools for selecting the optimal ADC for each patient.

The protocol OASIS (NCT pending) aims to define optimal assays for predicting resistance to several approved ADCs in patients treated according to standard indications. The OASIS-ImmunoPET protocol will explore the potential contribution of advanced molecular imaging to identify response to treatment in patients enrolled in the OASIS study.

The advent of ImmunoPET combines the high sensitivity and resolution of positron emission tomography (PET) with the specificity of monoclonal antibodies (mAb) or mAb-associated fragments used as radiotracers and as such provides a comprehensive characterization of the target distribution and the target engagement over the whole-body tumor burden labeling. ⁸⁹Zr is by far the most frequently used positron-emitting nuclide for antibody.

Gebhart et al. (2016) showed the value of adding [⁸⁹Zr]-Trastuzumab PET imaging to biopsies for the assessment of intrapatient tumor heterogeneity and for prediction of the treatment outcome in HER2-positive breast cancer patients treated with Trastuzumab emtansine (T-DM1).

This OASIS-ImmunoPET study is considered a pilot sub-study of OASIS and is designed to evaluate the association between [⁸⁹Zr]-Trastuzumab PET and response in patients with HER2-positive and HER2-low breast cancer treated with T-DXd, an ADC that was developed to target HER2-expressing cancers. We aim to explore whether resistance to T-DXd is dependent on target antigen loss or heterogeneity across tumor sites and whether there is a correlation between HER2 expression level on the tumor biopsy as determined by immunohistochemistry (IHC) and HER2 PET to understand whether Ab-radiolabelled PET scan can be used as a surrogate of tumor biopsy or treatment outcome.

Both [⁸⁹Zr]-Trastuzumab PET imaging and control FDG-PET imaging will be performed at two time points: at Baseline and at End of treatment (i.e. discontinuation for any reason). Additional control FDG-PET alone will be performed just before cycle 2 (for evaluation early response), and then every 3 cycles according to standard routine.

Conditions

Locally Advanced or Metastatic Breast Cancer

Keywords

Advanced molecular imaging; ADC; Response

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: NONE

Study Groups

Locally advanced or metastatic breast cancer treated with Trastuzumab deruxtecan (T-DXd)

Experimental advanced molecular imaging (ImmunoPET) combining the high sensitivity and resolution of positron emission tomography (PET) with the specificity of ADC target specific monoclonal antibodies.

Group Type: EXPERIMENTAL

Immuno-PET

Intervention Type: PROCEDURE

Realization of an advanced molecular imaging exam to visualize the T-DXd target (HER2) expression throughout the body, at baseline and at treatment discontinuation. This procedure relies on the administration to the patient of a radioactive tracer [89Zr]-anti-HER2.

This experimental imaging is associated to standard FDG-PET scans for tumor assessment.

Interventions

Immuno-PET

Realization of an advanced molecular imaging exam to visualize the T-DXd target (HER2) expression throughout the body, at baseline and at treatment discontinuation. This procedure relies on the administration to the patient of a radioactive tracer [89Zr]-anti-HER2.

This experimental imaging is associated to standard FDG-PET scans for tumor assessment.

Intervention Type: PROCEDURE

Eligibility Criteria

Inclusion Criteria

1. Patient must have signed a written informed consent prior to any trial specific procedures. When the patient is physically unable to give their written consent, a trusted person of their choice, independent from the investigator or the sponsor, can confirm in writing the patient's consent;

2. Patients enrolled in the prospective cohort of the OASIS study;

3. Patient with locally advanced or metastatic breast cancer eligible to receive T-DXd as part of their standard care;

4. At baseline imaging at least two "target" lesions fulfilling the following criteria: (1) anatomically transaxial diameter ≥ 1.5 cm and measurable per RECIST1.1. and (2) metabolically assessable with a maximum standard uptake value corrected for lean body mass (SUVmax) ≥ 1.5 x SUVmean + 2 standard deviations (SD) of the liver measured in a 3-cm-diameter spherical volume of interest (VOI) in normal liver parenchyma;

5. Patients must be willing and able to comply with the protocol for the duration of the trial;

Exclusion Criteria

1. Patients already treated with Trastuzumab deruxtecan (T-DXd);

2. Hypersensitivity at the ImmunoPET radioligands injection;

3. Patients who are claustrophobic or unable to remain still for 30 minutes;

4. Female participant who is pregnant, breastfeeding, or planning to become pregnant while enrolled in this study or within 90 days after the final administration of study treatment;

5. Person deprived of their liberty or under protective custody or guardianship.

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Jules Bordet Institute

OTHER

Sponsor Role: collaborator

UNICANCER

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Barbara Pistilli, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Gustave Roussy Cancer Center

Central Contacts

Marjorie Mauduit

Role: CONTACT

Phone: 0630481792

Email: m-mauduit@unicancer.fr

Jérôme Lemonnier

Role: CONTACT

Email: j-lemonnier@unicancer.fr

Other Identifiers

2025-524369-26-00

Identifier Type: OTHER

Identifier Source: secondary_id

UC-TRA-2521

Identifier Type: -

Identifier Source: org_study_id