Effect of Treatment With Butyric Acid in Anorexia Nervosa

NCT ID: NCT07289581

Last Updated: 2025-12-17

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

ACTIVE_NOT_RECRUITING

Clinical Phase

NA

Total Enrollment

50 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-11-12

Study Completion Date

2028-11-12

Brief Summary

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This clinical trial aims to evaluate the therapeutic potential of oral butyric acid supplementation in individuals with anorexia nervosa (AN). T The study will assess whether butyric acid, administered alongside standard multidisciplinary care, improves weight restoration and contributes to overall clinical recovery.

The primary objectives are to determine:

\- Whether butyric acid enhances weight restoration during the first 3 months of treatment.

Participants will be randomly assigned to receive either butyric acid or a placebo for 3 months, in addition to treatment-as-usual, which includes nutritional rehabilitation and cognitive-behavioral therapy.

Throughout the study, researchers will monitor:

* Changes in body weight and Body Mass Index (BMI)
* General and eating-disorder-related psychopathology
* Blood-based biomarkers related to inflammation, metabolism, and neuroendocrine function
* Gut microbiota composition and metabolites, including short-chain fatty acids

A total of 50 participants with AN are expected to be enrolled.

Detailed Description

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Conditions

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Anorexia Nervosa Gut Microbiota Weight Trajectory

Keywords

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anorexia nervosa gut microbiota butyric acid outcome

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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Active Comparator: Butyric Acid

Participants with anorexia nervosa receiving butyric acid supplementation plus Treatment As Usual.

Group Type ACTIVE_COMPARATOR

Dietary Supplement: Butyric Acid

Intervention Type DIETARY_SUPPLEMENT

Daily oral supplementation with 2 capsules of microencapsulated butyric acid (Butyrose®, 550 mg each; total daily dose 1,100 mg) for 3 months, administered in addition to Treatment As Usual (nutritional rehabilitation + cognitive-behavioral therapy).

Placebo Comparator: Placebo

Participants with anorexia nervosa receiving placebo capsules plus Treatment As Usual.

Group Type PLACEBO_COMPARATOR

Dietary Supplement: Butyric Acid

Intervention Type DIETARY_SUPPLEMENT

Daily oral supplementation with 2 placebo capsules matched in appearance and composition (maltodextrins, cellulose, inert excipients), administered for 3 months in addition to Treatment As Usual.

Interventions

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Dietary Supplement: Butyric Acid

Daily oral supplementation with 2 capsules of microencapsulated butyric acid (Butyrose®, 550 mg each; total daily dose 1,100 mg) for 3 months, administered in addition to Treatment As Usual (nutritional rehabilitation + cognitive-behavioral therapy).

Intervention Type DIETARY_SUPPLEMENT

Dietary Supplement: Butyric Acid

Daily oral supplementation with 2 placebo capsules matched in appearance and composition (maltodextrins, cellulose, inert excipients), administered for 3 months in addition to Treatment As Usual.

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* Female sex
* Age 18-65 years
* Current diagnosis of Anorexia Nervosa (DSM-5)
* Able and willing to provide informed consent
* Ability to comply with study procedures and assessments

Exclusion Criteria

* Severe psychiatric conditions requiring inpatient hospitalization (e.g., psychosis, severe major depressive episode with suicidal intent)
* Severe medical conditions requiring inpatient care (cardiovascular, renal, hematopoietic, gastrointestinal, endocrine disorders)
* Intellectual disability, illiteracy, or conditions impairing consent or questionnaire completion
* Current participation in another experimental protocol
* Active use of probiotics
* Neutropenia (\<0.5 × 10\^9/L)
* Leukocytosis (\>30 × 10\^9/L)
* Fever \> 38°C
* Radiological evidence of toxic megacolon or intestinal perforation
* Presence of colostomy
* Severe comorbidity limiting 30-day survival
* Active or past malignancy requiring chemotherapy
* Corticosteroid therapy within the past 6 months
* HIV infection
Minimum Eligible Age

18 Years

Maximum Eligible Age

65 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Azienda Ospedaliero-Universitaria Careggi

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Giovanni Castellini, Medical Degree

Role: PRINCIPAL_INVESTIGATOR

Department of Health Sciences, University of Florence, Florence, Italy

Locations

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SODc Psichiatria, Azienda Ospedaliero-Universitaria Careggi

Florence, FI, Italy

Site Status

Countries

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Italy

References

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Quagebeur R, Dalile B, Raes J, Van Oudenhove L, Verbeke K, Vrieze E. The role of short-chain fatty acids (SCFAs) in regulating stress responses, eating behavior, and nutritional state in anorexia nervosa: protocol for a randomized controlled trial. J Eat Disord. 2023 Oct 26;11(1):191. doi: 10.1186/s40337-023-00917-6.

Reference Type BACKGROUND
PMID: 37884972 (View on PubMed)

Mack I, Cuntz U, Gramer C, Niedermaier S, Pohl C, Schwiertz A, Zimmermann K, Zipfel S, Enck P, Penders J. Weight gain in anorexia nervosa does not ameliorate the faecal microbiota, branched chain fatty acid profiles, and gastrointestinal complaints. Sci Rep. 2016 May 27;6:26752. doi: 10.1038/srep26752.

Reference Type BACKGROUND
PMID: 27229737 (View on PubMed)

Hata T, Miyata N, Takakura S, Yoshihara K, Asano Y, Kimura-Todani T, Yamashita M, Zhang XT, Watanabe N, Mikami K, Koga Y, Sudo N. The Gut Microbiome Derived From Anorexia Nervosa Patients Impairs Weight Gain and Behavioral Performance in Female Mice. Endocrinology. 2019 Oct 1;160(10):2441-2452. doi: 10.1210/en.2019-00408.

Reference Type BACKGROUND
PMID: 31504398 (View on PubMed)

Facchin S, Vitulo N, Calgaro M, Buda A, Romualdi C, Pohl D, Perini B, Lorenzon G, Marinelli C, D'Inca R, Sturniolo GC, Savarino EV. Microbiota changes induced by microencapsulated sodium butyrate in patients with inflammatory bowel disease. Neurogastroenterol Motil. 2020 Oct;32(10):e13914. doi: 10.1111/nmo.13914. Epub 2020 May 31.

Reference Type RESULT
PMID: 32476236 (View on PubMed)

Castellini G, Cassioli E, Vitali F, Rossi E, Dani C, Melani G, Flaccomio D, D'Andria M, Mejia Monroy M, Galli A, Cavalieri D, Ricca V, Bartolucci GL, De Filippo C. Gut microbiota metabolites mediate the interplay between childhood maltreatment and psychopathology in patients with eating disorders. Sci Rep. 2023 Jul 20;13(1):11753. doi: 10.1038/s41598-023-38665-x.

Reference Type RESULT
PMID: 37474544 (View on PubMed)

Other Identifiers

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29252

Identifier Type: REGISTRY

Identifier Source: secondary_id

29252_spe

Identifier Type: -

Identifier Source: org_study_id