Neurokinin A and Matrix Metalloproteinase 9 Levels in Gingival Crevicular Fluid Samples of Teeth With Symptomatic Irreversible Pulpitis With Symptomatic Apical Periodontitis

NCT ID: NCT07289074

Last Updated: 2025-12-17

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Total Enrollment

23 participants

Study Classification

OBSERVATIONAL

Study Start Date

2026-02-28

Study Completion Date

2026-11-30

Brief Summary

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The aim of this study is to investigate the levels Neurokinin A and of Matrix Mtalloproteinase (MMP-9) in gingival crevicular fluid samples (GCF) as potential biomarkers for the diagnosis of symptomatic irreversible pulpitis with symptomatic apical periodontitis and to correlate the level of these diagnostic biomarkers ( Nerokinin A and MMP-9) with the pre-operative pain severity using the Numerical Rating Scale (NRS).

This study will utilize a comparative cross-sectional design to assess the levels of Neurokinin A (NKA) and Matrix metalloproteinase-9 (MMP-9) in gingival crevicular fluid (GCF) samples from symptomatic and healthy contralateral teeth with normal pulp.

Detailed Description

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The study design is Comparative Cross sectional study. The study will be conducted at the Dental hospital of Cairo University with access to patients undergoing treatment for teeth with pulpitis and apical periodontitis.

Conditions

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Symptomatic Irreversible Pulpitis With Apical Peridontitis

Keywords

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Gingival crevicular fluid Matrix Metalloproteinase 9 Neurokinin A

Study Design

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Observational Model Type

OTHER

Study Time Perspective

CROSS_SECTIONAL

Study Groups

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Patients diagnosed with symptomatic irreversible pulpitis with symptomatic apical peridontitis

* Gingival crevicular fluid samples will be collected from the symptomatic teeth and from the healthy contalateral teeth with normal pulp to detect the lavel of Neurokinin A (NKA) and Matrix metalloproteinase-9 (MMP-9) in the diseased teeth and compare it with that of the healthy teeth.
* The study will also correlate the level of these biomarkers with the preoperative pain severity using the Numerical Rating Scale (NRS).

Gingival crevicular fluid samples from the symptomatic teeth and the healthy contrlateral teeth

Intervention Type DIAGNOSTIC_TEST

The detection of the inflammatory biomarkers levels in the gingival crevicular fluid of the symptomatic teeth is considered an objective,molecular-based and non invasive method for diagnosis.

The healthy contralateral teeth of tha same patients

* Gingival crevicular fluid samples will be collected from the symptomatic teeth and from the healthy contalateral teeth to detect the lavel of Neurokinin A (NKA) and Matrix metalloproteinase-9 (MMP-9) in the diseased teeth and compare it with that of the healthy contralateral teeth.
* The study will also correlate the level of these biomarkers and the preoperative pain severity using the Numerical Rating Scale (NRS).

Gingival crevicular fluid samples from the symptomatic teeth and the healthy contrlateral teeth

Intervention Type DIAGNOSTIC_TEST

The detection of the inflammatory biomarkers levels in the gingival crevicular fluid of the symptomatic teeth is considered an objective,molecular-based and non invasive method for diagnosis.

Interventions

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Gingival crevicular fluid samples from the symptomatic teeth and the healthy contrlateral teeth

The detection of the inflammatory biomarkers levels in the gingival crevicular fluid of the symptomatic teeth is considered an objective,molecular-based and non invasive method for diagnosis.

Intervention Type DIAGNOSTIC_TEST

Other Intervention Names

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Matrix Metalloproteinase 9 and Neurokinin A will be detected in gingival crevicular fluid samples by using ELISA kits correlate the level of these diagnostic biomarkers with the pre-operative pain severity by using the numerical rating scale

Eligibility Criteria

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Inclusion Criteria

* Adults (aged 18-60) diagnosed with symptomatic irreversible pulpitis with symptomatic apical periodontitis and have healthy contralateral teeth with normal pulp.
* The involved teeth should have healthy periodontal conditions (Normal periodontal pocket depth, No bleeding on Probing and No mobility).
* Patients who have not undergone any previous dental treatments in the involved teeth.
* Signed informed consent.

Exclusion Criteria

* Patients with systemic diseases affecting the immune response (e.g., autoimmune diseases, uncontrolled diabetes).
* Patients taking medications that can affect the inflammatory markers (e.g., Steroids and Tetracyclins).
* Smokers.
* Individuals with periodontal disease unrelated to apical periodontitis.
* Presence of marginal bone loss.
* Pregnant women.
Minimum Eligible Age

18 Years

Maximum Eligible Age

60 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Hadeer Mostafa El Mohamady El Feky

OTHER

Sponsor Role lead

Responsible Party

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Hadeer Mostafa El Mohamady El Feky

PhD Candidate at Faculty of Dentistry- Endodontic Department (Cairo univerity)

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Saied M Abdel Aziz, Professor of Endodontics

Role: STUDY_DIRECTOR

Faculy of Dentistry, Cairo university, Egypt

Locations

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Faculty of Dentistry, Cairo university

Cairo, Cairo Governorate, Egypt

Site Status

Countries

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Egypt

Central Contacts

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Hadeer M El Mohamady El Feky, BDS, MSc, PhD Candidate

Role: CONTACT

Phone: +201029997864

Email: [email protected]

Facility Contacts

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Hadeer M EL Mohamady EL Feky, BDS, MSc, PhD Candidate

Role: primary

Other Identifiers

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Hadeer Mostafa -14422022580639

Identifier Type: -

Identifier Source: org_study_id