Impact of Guided Biofilm Therapy on Epithelial and Mesenchymal Biomarkers in Periodontitis Patients

NCT ID: NCT07286916

Last Updated: 2025-12-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Clinical Phase

NA

Total Enrollment

18 participants

Study Classification

INTERVENTIONAL

Study Start Date

2026-01-31

Study Completion Date

2026-06-30

Brief Summary

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The goal of this clinical trial is to investigate the clinical and biochemical effects of the guided biofilm therapy protocol as an alternative to the conventional non-surgical periodontal therapy in patients with periodontitis. The main question it aims to answer is:

Does the guided biofilm therapy (GBT) protocol have an impact on gingival crevicular fluid (GCF) epithelial and mesenchymal biomarkers in patients with periodontitis?

All patients will be instructed to brush their teeth twice daily and will be supplied with the same type of toothpaste and toothbrush, with suitable interdental aids. All patients will be instructed to attend again after 7 days.

At baseline, full-mouth periodontal charting and radiographs will be obtained and after 1-hour, GCF samples will be collected from both test and control groups. Sites allocated to control group will undergo supragingival debridement by using ultrasonic device. After one week, the control group will receive RSD using area specific (Gracey) curettes (Medesy, Italy) according to the 24-hours protocol and will receive teeth polishing by using rubber polishing cups and tooth polishing paste, while the test group will receive a full GBT protocol which includes eight steps: (1) ASSESS, (2) DISCLOSE, (3) MOTIVATE, (4) AIRFLOW, (5) PERIOFLOW, (6) PIEZON PS\*, (7) CHECK, and (8) RECALL. After finishing the treatment session, the patients will be asked to attend for follow up, clinical evaluation of PI, BOP, PPD, and CAL, and GCF samples collection after 1 month and 3 months.

Detailed Description

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Conditions

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Periodontitis

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

The study is a 2 arm, split-mouth, double blinded, randomized clinical trial. The first group is control group will receive conventional NSPT (scaling \& root surface debridement (RSD)), while the second group is the test group will be treated according to the GBT protocol.
Primary Study Purpose

TREATMENT

Blinding Strategy

SINGLE

Outcome Assessors

Study Groups

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Conventional non-surgical periodontal therapy (NSPT)

Control group

Group Type ACTIVE_COMPARATOR

Root surface debridement

Intervention Type PROCEDURE

Control group sites that will receive conventional NSPT will undergo supragingival debridement by using ultrasonic device at baseline. After one week, sites will receive root surface debridement (RSD) using area specific (Gracey) curettes (Medesy, Italy) according to the 24-hours protocol.

Guided Biofilm Therapy (GBT)

Test group

Group Type EXPERIMENTAL

Guided Biofilm Therapy

Intervention Type DEVICE

Test group sites will receive guided biofilm therapy (GBT) protocol using the Airflow Prophylaxis Master device by EMS which includes EMS DISCLOSING AGENT making the biofilm visible, EMS AIRFLOW combined with PLUS erythritol powder for removing biofilm, discoloration, and early calculus, PERIOFLOW removing the biofilm in deep periodontal pockets gently and thoroughly, and finally PIEZON PS for removing the remaining calculus.

Interventions

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Root surface debridement

Control group sites that will receive conventional NSPT will undergo supragingival debridement by using ultrasonic device at baseline. After one week, sites will receive root surface debridement (RSD) using area specific (Gracey) curettes (Medesy, Italy) according to the 24-hours protocol.

Intervention Type PROCEDURE

Guided Biofilm Therapy

Test group sites will receive guided biofilm therapy (GBT) protocol using the Airflow Prophylaxis Master device by EMS which includes EMS DISCLOSING AGENT making the biofilm visible, EMS AIRFLOW combined with PLUS erythritol powder for removing biofilm, discoloration, and early calculus, PERIOFLOW removing the biofilm in deep periodontal pockets gently and thoroughly, and finally PIEZON PS for removing the remaining calculus.

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

1. Adult subjects (\>18 years).
2. Subjects are not currently under active periodontal therapy or joining other trial in the last 3 months.
3. Patients must be diagnosed with generalized unstable periodontitis with bilateral, symmetrical distribution of PPD ≥ 4mm and positive BOP.

\-

Exclusion Criteria

1. Patients not diagnosed with periodontitis.
2. Patients consuming antibiotics.
3. Regular users of nonsteroidal anti-inflammatory drugs (NSAIDs).
4. Patients receiving periodontal treatment 3-months prior to the study.
5. Pregnant or mothers in a breastfeeding period.
6. Third molars and teeth with furcation involvement.
7. Patients taking antioxidant supplements.
8. Patients with heavy calculus that would prevent PerioPaper strips entrance to the periodontal pocket.
9. Patient with pulmonary diseases and with a history of allergy to erythritol.
10. Patients not willing to participate. -
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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University of Baghdad

OTHER

Sponsor Role lead

Responsible Party

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Ibrahim Haider Sadeq

Dentist

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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College of Dentistry, University of Baghdad

Baghdad, , Iraq

Site Status

Countries

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Iraq

Central Contacts

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Ibrahim Haider Sadeq, B.D.S

Role: CONTACT

009647722247642

Facility Contacts

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College of Dentistry, University of Baghdad

Role: primary

0096414169394

Other Identifiers

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1108625

Identifier Type: -

Identifier Source: org_study_id