Tumor Integrating Gut Microbiota in bREast Cancer

NCT ID: NCT07253168

Last Updated: 2025-11-28

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Clinical Phase

NA

Total Enrollment

240 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-12-01

Study Completion Date

2028-12-01

Brief Summary

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Study on patients undergoing surgery for breast cancer. Objective: to characterize the intratumoral microbiota and integrate these findings with clinicopathologic data, the estrobolome, and gut microbiota, with analyses stratified by molecular subtype (Luminal A, Luminal B, HER2-enriched \[human epidermal growth factor receptor 2-enriched\], and TNBC \[triple-negative breast cancer\]).

Enrollment: consecutive recruitment in the operating room based on the availability of biopsy/histological material.

Samples: tumor tissue, adjacent normal tissue; urine (for indirect assessment of estrobolome activity); and stool. Strict anti-contamination measures will be implemented.

Exploratory outcomes: differences across subtypes, associations with features of the TME (tumor microenvironment), including TILs (tumor-infiltrating lymphocytes), and markers of progression or treatment resistance

Detailed Description

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Conditions

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Breast Cancer

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

OTHER

Blinding Strategy

NONE

Study Groups

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Patients undergoing surgery for breast cancer

single-arm discovery study with consecutive enrollment of eligible patients undergoing surgery for primary breast cancer

Group Type EXPERIMENTAL

Surgery for breast cancer

Intervention Type PROCEDURE

To investigate the intratumoral microbiota in breast cancer and integrate these findings with gut microbiota analyses, including estrobolome characterization, to explore potential microbial-host interactions influencing tumor biology across molecular subtypes

Interventions

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Surgery for breast cancer

To investigate the intratumoral microbiota in breast cancer and integrate these findings with gut microbiota analyses, including estrobolome characterization, to explore potential microbial-host interactions influencing tumor biology across molecular subtypes

Intervention Type PROCEDURE

Other Intervention Names

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Characterization of intratumoral microbiota

Eligibility Criteria

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Inclusion Criteria

* Women ≥18 years with primary, non-metastatic breast cancer (BC) undergoing surgery.
* Availability of sufficient tumor and adjacent normal tissue for research purposes after completion of diagnostic sampling.
* Written informed consent

Exclusion Criteria

* Active systemic infection or fever \>38.0°C within 72 h pre-surgery.
* Antibiotics within 14 days pre-sampling (recorded; analyses adjusted).
* Prior ipsilateral BC surgery for current lesion (except re-excision with fresh tissue).
* Any condition preventing compliance with the study protocol.
Minimum Eligible Age

18 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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Fondazione Policlinico Universitario Agostino Gemelli IRCCS

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Brunella Posteraro

Role: PRINCIPAL_INVESTIGATOR

Fondazione Policlinico Universitario A. Gemelli, IRCCS

Central Contacts

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Brunella Posteraro

Role: CONTACT

+3906030157959

Other Identifiers

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8051

Identifier Type: -

Identifier Source: org_study_id

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