Secretome Treatment for Facial Rejuvenation

NCT ID: NCT07227883

Last Updated: 2025-11-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Clinical Phase

EARLY_PHASE1

Total Enrollment

24 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-11-25

Study Completion Date

2026-11-15

Brief Summary

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Secretome treatment for facial rejuvenation

Detailed Description

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This study will quantitatively evaluate the efficacy and safety of the autologous hair follicle secretome for skin rejuvenation. The secretome is produced by non-invasively collecting hair follicles by plucking 50-75 hairs. Ten follicles are selected to cultured in media (proprietary xeno- and human protein free) for 4 weeks and conditioned media is collected and lyophilized to produce the autologous secretome product. The secretome contains growth factors, extracellular matrix molecules, cytokines, exosomes, etc. Upon use, the autologous secretome is diluted in saline and used on the subject the hairs were collected from. This study is evaluating the efficacy and safety of intradermal injections of the secretome for facial rejuvenation. The study is a double-blind and open label extension phases, randomized, placebo-controlled trial. Double-blind up to 120 days, followed by an open label extension for control patients opting to cross-over and receive active treatment.

Conditions

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Facial Aging Rejuvenation Stem Cell Stem Cell Banking

Keywords

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stem cells secretome autologous hair follicle aging facial face under eyes wrinkles anti-aging rejuvenation regeneration

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Optional cross-over after blinded phase of study
Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors
Sponsor, except for clinical study managers

Study Groups

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Control

Saline will act as a control and will be intradermally injected.

Group Type PLACEBO_COMPARATOR

Control Saline

Intervention Type OTHER

Saline solution will be the placebo control. The active is diluted with saline so this is an appropriate control.

Active-Secretome

Autologous Hair follicle Derived Secretome

Group Type EXPERIMENTAL

Autologous Hair Follicle-Derived Secretome

Intervention Type OTHER

This interventional product is a secretome derived from the stem cells that reside in the hair follicle.

Interventions

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Autologous Hair Follicle-Derived Secretome

This interventional product is a secretome derived from the stem cells that reside in the hair follicle.

Intervention Type OTHER

Control Saline

Saline solution will be the placebo control. The active is diluted with saline so this is an appropriate control.

Intervention Type OTHER

Other Intervention Names

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aHFS Hair Follicle Secretome Hair Follicle Stem Cell

Eligibility Criteria

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Inclusion Criteria

1. Male or Female
2. Age: ≥30 and ≤ 70 at the time of consent.
3. Fitzpatrick skin types I-V
4. Moderate to very severe facial aging according to the SASSQ for the categories of elasticity, wrinkles, and skin roughness.
5. Willing to refrain from cosmetic procedures throughout the study period (i.e. laser treatments, chemical peels, microneedling, dermal filler injections, neuromodulator injections, etc.)
6. Willing to use study support products as directed and will not introduce any new skin care or makeup products throughout the study period. Subjects will be provided with a Skincare Kit: Cetaphil Gentle Skin Cleanser 20 oz, Cetaphil Daily Hydrating Lotion 3 oz, Cetaphil Sheer Mineral Sunscreen Broad Spectrum SPF 50 1.7 oz. Two of each will be provided with application instructions.
7. Male subjects with facial hair must be willing to shave the morning of their treatments/follow-up visits.
8. Willing to minimize sun/ultraviolet light exposure for the duration of the study, including all of the following: applying sunscreen daily, at least 15 minutes before going outside; wearing sun-protective clothing (e.g., a hat, sunglasses) when outdoors; reapplying sunscreen every 2 hours during outdoor activities; avoiding tanning bed/tanning product use.


1. Male or Female
2. Age: ≥30 and ≤ 70 at the time of consent.
3. Fitzpatrick skin types I-V
4. Moderate to very severe facial aging according to the SASSQ for the categories of elasticity, wrinkles, and skin roughness.
5. Willing to refrain from cosmetic procedures throughout the study period (i.e. laser treatments, chemical peels, microneedling, dermal filler injections, neuromodulator injections, etc.)
6. Willing to use study support products as directed and will not introduce any new skin care or makeup products throughout the study period. Subjects will be provided with a Skincare Kit: Cetaphil Gentle Skin Cleanser 20 oz, Cetaphil Daily Hydrating Lotion 3 oz, Cetaphil Sheer Mineral Sunscreen Broad Spectrum SPF 50 1.7 oz. Two of each will be provided with application instructions.
7. Male subjects with facial hair must be willing to shave the morning of their treatments/follow-up visits.
8. Willing to minimize sun/ultraviolet light exposure for the duration of the study, including all of the following: applying sunscreen daily, at least 15 minutes before going outside; wearing sun-protective clothing (e.g., a hat, sunglasses) when outdoors; reapplying sunscreen every 2 hours during outdoor activities; avoiding tanning bed/tanning product use.
9. Competent and willing to provide written, informed consent to participate in all study activities
10. Willing and able to tolerate multiple injections and attend all study visits
11. Negative urine pregnancy test result at the time of screening (if applicable)
12. For female subjects of childbearing potential, must be willing to use an acceptable form of birth control during the entire course of the study. All systemic birth control measures must be in consistent use for at least 30 days prior to study enrollment. A female is considered of childbearing potential unless she is postmenopausal, without a uterus and/or both ovaries, or has had a bilateral tubal ligation.

Exclusion Criteria

1. Use of topical tretinoin, adapalene, tazarotene, hydroquinone, imiquimod, 5-fluorouracil, ingenol mebutate, concentrated hydrogen peroxide, diclofenac, vitamin C, alpha hydroxy acids, or photodynamic therapy (PDT) to the face within 4 weeks prior to baseline photography and throughout the study period
2. Dermatologic diseases or conditions (e.g. atopic dermatitis, psoriasis, rosacea, seborrheic dermatitis, wounds, infections, irritation, sunburn, windburn, etc.) in the treatment area that could interfere with the study, in the opinion of the Investigator
3. History of keloid formation, hypertrophic scarring, or abnormal/delayed wound healing
4. Previous medical aesthetic rejuvenation treatments on the face, such as chemical peel, dermabrasion, non-ablative or ablative resurfacing procedure, or face-lift with laser or other devices, up to 3 months prior to baseline photography
5. Treatment with neuromodulators in crow's feet, glabellar region, or frontalis muscles within 4 months prior to baseline photography
6. Treatment with dermal fillers, collagen stimulators, fat injections or permanent fillers within the past 6 months prior to baseline photography.
7. Patients using other anti-aging treatments/procedures/systemic medications or injections administered/prescribed by a medical professional before starting the study, if the investigator believes they will confound the results. Consulting with Sponsor is encouraged.
8. Heavy smoker (\>1 pack of cigarettes a day)
9. Energy-based device (i.e., radiofrequency device treatments, microfocused ultrasound device or other ultrasound-based device treatments, laser and light-based device treatments, microneedling) treatment in the treatment area within 6 months prior to baseline photography
10. Currently be taking isotretinoin (Accutane).
11. Subjects on an immunosuppressant or have an autoimmune condition (except stable, controlled Hashimoto's thyroiditis)
12. Surgery (i.e., face lift, eyebrow lift, neck lift or lower rhytidectomy, liposuction, etc.) during the 12-month period prior to baseline photography
13. Current participation or participation within 30 days prior to the start of this study in a drug or other investigational research study
14. Severe active infection (e.g. HIV, HepB or C, UTI, etc.)
15. Current or recent (within last 5 years) malignancy (except basal cell and squamous cell skin cancers as long as basal cell was not on the face)
16. History of systemic chemotherapy or radiation within the last 5 years
17. Continuous/daily use of nonsteroidal anti-inflammatory or Vitamin E, unless discontinued within 7 days before 1st treatment and only used as needed through the trial period.
18. Anticipated pregnancy or trying to become pregnant in the next year
19. Current anticoagulant therapy (heparins; factor Xa inhibitors; direct agents such dabigatran, rivaroxaban, apixaban, endoxaban and betrixaban; warfarin/coumarins). Low dose aspirin and omega-3 supplements allowed.
20. Any other criterion that based on the clinical judgment of the investigator may place the subject at risk or confound study results
21. Unable or unwilling to comply with the requirements (i.e. removal of jewelry, makeup, facial hair) of the study protocol in the opinion of the Investigator
22. Subject has any disorder that may prevent compliance, such as a history of chronic alcohol or drug abuse, significant mental or nervous disorder or other illness that would, in the evaluator's opinion, interfere with the study.

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Minimum Eligible Age

30 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Acorn Biolabs Inc.

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Steve Yoelin, MD

Newport Beach, California, United States

Site Status

Countries

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United States

Central Contacts

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Lisa M Campbell, PhD

Role: CONTACT

Phone: 905-695-6956

Email: [email protected]

Heather Dwyer, MSc, CCRP

Role: CONTACT

Phone: 905-695-6956

Email: [email protected]

Facility Contacts

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Margaret Xu

Role: primary

Steve Yoelin, MD

Role: backup

Other Identifiers

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ACN-301

Identifier Type: -

Identifier Source: org_study_id