Target Validation and Efficacy of Metformin in Patients With Posterior Fossa Group A (PFA) Ependymoma

NCT ID: NCT07226453

Last Updated: 2025-11-10

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Clinical Phase

PHASE2

Total Enrollment

30 participants

Study Classification

INTERVENTIONAL

Study Start Date

2026-02-28

Study Completion Date

2030-03-31

Brief Summary

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This is a multi-site study of the pharmacodynamic effects and efficacy of metformin in children and young adults with recurrent or progressive Posterior Fossa Group A (PFA) ependymoma.

Detailed Description

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PRIMARY OBJECTIVES:

I. To assess pharmacodynamic effect on PFA tumor cells of metformin in participants with recurrent or progressive PFA undergoing surgical resection of the tumor by determining change in H3K27me3 and/or EZHIP expression in tumor nuclei compared to pre-treatment levels (TV Phase)

II. To measure disease stabilization rate by Response Assessment in Pediatric Neuro-Oncology (RAPNO) criteria (complete (CR), partial (PR) response; ≥50% reduction in size but \<CR), and Stable (SD); \< 50% reduction in size but not meeting criteria for progressive disease) in participants with recurrent or progressive PFA ependymoma following metformin treatment (Efficacy Phase).

EXPLORATORY OBJECTIVES:

I. Estimate the 1-year progression-free survival and overall-survival of participants with recurrent or progressive PFA treated with single agent metformin (Efficacy Phase).

II. To assess pharmacokinetics (PK) of metformin by measuring plasma, CSF and intra-tumoral concentration of metformin, the latter two obtained at the time of surgery or at the time of further disease progression on study in consenting participants (Both cohorts).

III. Evaluate the feasibility of using MR spectroscopy (MRS) imaging to study oncometabolite profile of recurrent or progressive PFA ependymoma pre-and post-treatment with metformin.

OUTLINE:

This will be a two-part study: a TV phase and an efficacy phase. Participants will be initially enrolled in the TV phase and receive treatment prior to planned surgery. Participants already enrolled on the TV phase may continue to the efficacy phase of the study if they have measurable disease post-surgery. Participants in the efficacy phase will receive daily oral metformin until disease progression or till there are unacceptable adverse event(s). Long term/survival follow-up procedures are to be captured under the Pediatric Neuro-Oncology Consortium (PNOC) Protocol for Children and Young Adults Diagnosed with a Central Nervous System (CNS) Tumor to Assess Cognitive, Quality of Life (QOL), and Comprehensive Effects of Therapies protocol (COMP). Participants will be followed under the PNOC COMP protocol until death or withdrawal from study.

Conditions

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Posterior Fossa Ependymal Tumor

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SEQUENTIAL

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Target Validation (TV) Phase (Metformin)

Participants will each receive daily oral metformin with a weekly schedule starting at 250 mg/m\^2 twice a day (BID) for the first week, then increasing to 500 mg/m\^2 BID for week 2, 1000 mg/m\^2 BID for week 3, and then 1666 mg/m\^2 BID for week 4-6 prior to planned surgery. Participants with no measurable disease following surgical intervention may either go off treatment or continue metformin at the physician's discretion. Participants have the option of continuing on the efficacy phase of the study without undergoing planned surgery no demonstrated progressive disease on pre-surgery MRI AND if the family opts to continue treatment with metformin without undergoing surgical resection of the tumor. Participants with measurable tumor by RAPNO criteria following surgery may continue onto efficacy phase and receive maintenance metformin until disease progression or till there are unacceptable adverse event(s).

Group Type EXPERIMENTAL

Metformin

Intervention Type DRUG

Given orally (PO)

Planned Surgical Resection

Intervention Type PROCEDURE

Undergo planned surgery as part of regular care

Magnetic Resonance Imaging (MRI)

Intervention Type PROCEDURE

Undergo MRI imaging

Specimen Collection

Intervention Type PROCEDURE

Tumor Tissue, blood, and cerebral spinal fluid (CSF) may be collected for correlative analysis.

MR spectroscopy (MRS)

Intervention Type PROCEDURE

Undergo MRS imaging

Efficacy Phase (Metformin)

Participants with recurrent or progressive PFA ependymoma that have failed upfront surgery and radiation will receive daily oral metformin until disease progression or till there are unacceptable adverse event(s). Disease response will be assessed by MRI imaging using RAPNO criteria. Participants already enrolled on the TV phase may enroll in this phase if they have measurable disease post-surgery. Treatment may continue until disease progression or until there are unacceptable adverse event(s).

Group Type EXPERIMENTAL

Metformin

Intervention Type DRUG

Given orally (PO)

Planned Surgical Resection

Intervention Type PROCEDURE

Undergo planned surgery as part of regular care

Magnetic Resonance Imaging (MRI)

Intervention Type PROCEDURE

Undergo MRI imaging

Specimen Collection

Intervention Type PROCEDURE

Tumor Tissue, blood, and cerebral spinal fluid (CSF) may be collected for correlative analysis.

MR spectroscopy (MRS)

Intervention Type PROCEDURE

Undergo MRS imaging

Interventions

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Metformin

Given orally (PO)

Intervention Type DRUG

Planned Surgical Resection

Undergo planned surgery as part of regular care

Intervention Type PROCEDURE

Magnetic Resonance Imaging (MRI)

Undergo MRI imaging

Intervention Type PROCEDURE

Specimen Collection

Tumor Tissue, blood, and cerebral spinal fluid (CSF) may be collected for correlative analysis.

Intervention Type PROCEDURE

MR spectroscopy (MRS)

Undergo MRS imaging

Intervention Type PROCEDURE

Other Intervention Names

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Glucophage Surgical Resection MRI Specimen Sample Collection MRS

Eligibility Criteria

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Inclusion Criteria

1. Participants must have recurrent or progressive posterior fossa A (PFA) ependymoma following surgery AND radiation treatment (RT).
2. Participants must have a diagnosis of PFA ependymoma either at initial diagnosis or at recurrence. Any number of previous recurrences are permissible provided the participant meets other enrollment criteria.
3. Participants must have adequate tumor tissue available from initial diagnosis or from pre- trial enrollment. Formalin-fixed paraffin-embedded (FFPE) material (1 full block) should be provided. If FFPE material is not available, 10 unstained slides with an accompanying hematoxylin and eosin (H\&E) report should be provided.

Target Validation (TV) Phase:

o Participants are candidates to undergo elective surgery for removal of all or a portion of their recurrent/progressive tumor.

Efficacy Phase:
* Participant must have measurable disease; this will be defined as lesions that can be accurately measured in two dimensions (longest diameter to be recorded) The size threshold is met if both in-plane diameters are ≥10 mm or both in-plane diameters are at least two times the MRI slice thickness, plus the interslice gap with a minimum size of no less than double the slice thickness on MRI.
* Participants with an isolated local progression of the tumor following RT (or stereotactic radiosurgery, SRS) must be \> 6 months from completion of RT to the lesion to rule out pseudo progression or must have tissue confirmation of progression prior to enrollment.
* Previously irradiated lesions are considered non-measurable except in cases of documented progression of the lesion since the completion of radiation therapy.
4. Prior Therapy: Participants must not be receiving metformin for other medical indications or previous exposure to metformin following their diagnosis of PFA ependymoma. However, participants treated on the TV phase, but did not continue onto maintenance therapy will be allowed to enroll on the efficacy phase with future recurrences or progression of their disease.
5. Age: 1 -39 years at the time of enrollment.
6. Performance Score: Karnofsky \>= 50 for participants \> 16 years of age and Lansky \>=50 for participants \<=16 years of age. Participants who are unable to walk because of paralysis, but who are up in a wheelchair, will be considered ambulatory for the purpose of assessing the performance score.
7. Corticosteroids: Participants who are receiving dexamethasone must be on a stable or decreasing dose for at least 1 week prior to registration.
8. Organ Function Requirements

1. Peripheral absolute neutrophil count (ANC) \>= 1000/mm\^3.
2. Platelet count \>=100,000/mm\^3 (transfusion independent, defined as not receiving platelet transfusions for at least 7 days prior to enrollment).
3. Serum creatinine \< 1.5 Upper Limit normal (ULN) based on age and gender
4. Total bilirubin \<= 1.5 x ULN for age; in presence of Gilbert's syndrome, total bilirubin \< 3 x ULN or direct bilirubin \< 1.5 x ULN
5. alanine aminotransferase (ALT) \<= 3 x ULN.
6. aspartate aminotransferase (AST) \<=3 x ULN.
9. Participants with seizure disorder may be enrolled if well controlled and are on stable dose of anti-seizure medication for \> 72 hours prior to enrollment. Participants who have neurological deficits should have deficits that are stable for a minimum of 1 week prior to registration.
10. Participants must enroll on PNOC COMP if PNOC COMP is open to accrual at the enrolling institution.
11. A legal parent/guardian or participant must be able to understand, and willing to sign, a written informed consent and assent document, as appropriate.

Exclusion Criteria

1. Participants with a history of diabetes mellitus or those found to have pre-diabetes on HbA1C screening test are excluded from the study.
2. Participants without any measurable disease but with only isolated leptomeningeal disease progression.
3. Participants who have had chemotherapy or have received radiotherapy to the non-target lesion within 3 weeks (6 weeks for nitrosoureas or mitomycin C) prior to entering the study or those who have not recovered from adverse events due to agents administered more than 4 weeks earlier.
4. Participants must be at least 7 days since the completion of therapy with a biologic or small molecule agent. For any agent with known adverse events that can occur beyond 7 days after administration, the period prior to enrollment must be beyond the time during which adverse events are known to occur. Such participants should also be discussed with study chairs.
5. Participants with rapidly progressive symptoms that require urgent surgery that in the investigators assessment cannot be safely deferred for 6 weeks are excluded from target validation phase of the study
6. Participants who are receiving any other investigational agents.
7. History of allergic reactions attributed to compounds of similar chemical or biologic composition to metformin.
8. Uncontrolled intercurrent illness including, but not limited to, ongoing or active infection.
9. Women of childbearing potential must not be pregnant or breast-feeding.
10. Human immunodeficiency virus- (HIV) positive participants will be ineligible if HIV therapy regimen has not been stable for at least 4 weeks or there is intent to change the regimen within 8 weeks following enrollment, or if they are severely immunocompromised.
Minimum Eligible Age

1 Year

Maximum Eligible Age

39 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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The Lilabean Foundation, Inc.

UNKNOWN

Sponsor Role collaborator

Pediatric Neuro-Oncology Consortium

OTHER

Sponsor Role collaborator

University of California, San Francisco

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Sabine Mueller, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

University of California, San Francisco

Santhosh A Upadhyaya, MD

Role: STUDY_CHAIR

University of Michigan, C. S. Mott Children's Hospital

Locations

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University of California, San Francisco

San Francisco, California, United States

Site Status

Countries

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United States

Central Contacts

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Denise Truong

Role: CONTACT

Phone: 415-502-1600

Email: [email protected]

Facility Contacts

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Denise Truong

Role: primary

Other Identifiers

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NCI-2025-08332

Identifier Type: REGISTRY

Identifier Source: secondary_id

25087

Identifier Type: -

Identifier Source: org_study_id