Integrative Diagnosis for SCD and Other RADs

NCT ID: NCT07206095

Last Updated: 2025-10-03

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

200 participants

Study Classification

OBSERVATIONAL

Study Start Date

2020-11-13

Study Completion Date

2028-05-31

Brief Summary

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INTEGRA aims at enabling personalized medicine for RHADs patients by the establishment of an integrative diagnostic approach based on deep phenotypic and genetic characterization through combining new generation methodologies.

Detailed Description

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Objectives:

* To assess the prognostic value of LoRRca (ektacytometry) as biomarker providing information of SCD/RADs patients severity
* To investigate the correlation between LoRRca parameters and SCD/RADs patients genetic and phenotypic characterization.
* To identify genetic modifiers of RADs both new and previously described by GWAS as markers for prognosis and clinical course based on genomics approach.
* To establish an innovative algorithm for RADs patients characterization based on the integration of data generated through the analysis of genetic modifiers and the RBCs rheological properties by LoRRca profiles and microfluidics data in combination with RADs patients' clinical manifestations and treatments.
* To model the progression of RADs in a spleen-like filtering unit using microfluidic technologies to develop a novel diagnostic device for prognosis and patients' stratification. This device will be used for the characterization under flow of rheological and mechanical properties of single RBCs.
* To translate the results on a clinical practice recommendation for management of RADs patients endorsed by European Hematology bodies as ERN-EuroBloodNet and/or the European Hematology Association for its wide dissemination.

Conditions

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Sickle Cell Disease Thalassaemia Congenital Dyserythropoietic Anemia (CDA) Enzyme Disorder; Anemia Spherocytosis, Hereditary Stomatocytosis Hemoglobin Disorder Anemia Due to Membrane Defect Rare Anemia Disorders

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Interventions

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Analysis of genetic modifiers

Genetic modifiers for rare anemia disorders will be analyzed through massive sequencing.

Intervention Type GENETIC

Disease phenotyping

Peripheral blood samples will be used for conventional phenotyping characterization including among others: RBCs morphology, fragility osmotic test, hemoglobin fraction and quantification, hemoglobin stability test, EMA binding test, RBC enzymes quantification assay, RBC rheological properties through Lorrca Maxsis Osmoscan/Oxygescan (Lorrca®)

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Patients sustaining a confirmed or suspected diagnosis of an hereditary rare hemolytic anemia:
* Sickle cell disease
* Thalassemic syndromes
* Congenital dyserythropoietic anemia
* Enzymopathy
* Unstable Hemoblogin / Altered oxygen affinity
* Hereditary stomatocytosis
* Hereditary pyropoikilocytosis
* Hereditary spherocytosis with severe anemia (\<8 g/dL) or inconclusive diagnosis:
* Patient with chronic hemolytic anemia and red cell smear compatible, but with:
* EMA binding test: inconclusive or negative
* Genetic testing: no definitive diagnosis (VUS or no findings)
* Not transplanted or undergoing gene therapy at the time of inclusion. Patients with graft failure without a new transplant may be included.

Exclusion Criteria

* Carrier traits in autosomal recessive hereditary anemias
Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Hospital Clinic de Barcelona, Barcelona, Spain

UNKNOWN

Sponsor Role collaborator

Institute for Bioengineering of Catalonia

OTHER

Sponsor Role collaborator

Hospital Arnau de Vilanova, Lleida (Spain)

UNKNOWN

Sponsor Role collaborator

Hospital Universitari Vall d'Hebron Research Institute

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Hospital de la Santa Creu i Sant Pau

Barcelona, Barcelona, Spain

Site Status RECRUITING

Hospital Universitari Vall d'Hebron

Barcelona, Barcelona, Spain

Site Status RECRUITING

Hospital Sant Joan de Déu

Esplugues de Llobregat, Barcelona, Spain

Site Status RECRUITING

Hospital General de Granollers

Granollers, Barcelona, Spain

Site Status RECRUITING

Consorci Sanitari del Maresme - Hospital de Mataró

Mataró, Barcelona, Spain

Site Status RECRUITING

Parc Taulí Hospital Universitari

Sabadell, Barcelona, Spain

Site Status RECRUITING

Hospital Universitari Mútua de Terrassa

Terrassa, Barcelona, Spain

Site Status RECRUITING

Consorci Sanitari de Terrassa

Terrassa, Barcelona, Spain

Site Status RECRUITING

Hospital Universitari Arnau de Vilanova

Lleida, Lleida, Spain

Site Status RECRUITING

Countries

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Spain

Central Contacts

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Mar Mañú Pereira PhD

Role: CONTACT

+34 93 489 4063

Facility Contacts

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Anna collado gimbert

Role: primary

680810602

Anna collado gimbert

Role: primary

680810602

Anna collado gimbert

Role: primary

680810602

Anna collado gimbert

Role: primary

680810602

Anna collado gimbert

Role: primary

680810602

Anna collado gimbert

Role: primary

680810602

Anna collado gimbert

Role: primary

680810602

Other Identifiers

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Evidence 860436

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

Integra-SCD PI20/01454

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

GA 101095530 - SYNTHEMA

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

GA 101017549 - GENOMED4ALL

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

PR(AMI)427/2021

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

PR(AMI)543/2020

Identifier Type: -

Identifier Source: org_study_id

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