Causal Mechanisms of Odor-Guided Behavior in Humans

NCT ID: NCT07099092

Last Updated: 2025-11-12

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Clinical Phase

EARLY_PHASE1

Total Enrollment

80 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-11-17

Study Completion Date

2035-07-14

Brief Summary

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Background:

Little is known about how different regions of the brain responsible for the human sense of smell guide behaviors. In this study, researchers use a technique called transcranial ultrasound stimulation (TUS) to learn how odors affect the brain and behavior.

Objective:

To learn more about how the human sense of smell works.

Eligibility:

Healthy people aged 18 to 45 years who are right-handed.

Design:

Participants can volunteer for up to 2 different experiments. Each experiment requires 5 visits, each about 1 week apart. Food, alcohol, and caffeine may be limited before visits.

At the start of each visit, participants will answer questions about their health and how well they slept. Their sense of smell will be assessed.

Some visits may include tasks on a computer: While doing these tasks, participants may be asked to smell different odors, look at pictures, and listen to sounds. They will wear devices to track breathing, blood pressure, pulse, and other body responses to the tasks.

Some visits may include TUS: TUS uses ultrasound waves to briefly change brain activity. A gel will be applied to the scalp and hair, and a device will be placed against the participant s head. Participants may feel a tapping, pulling, and/or warm sensation on the skin underneath the device. They may also feel a twitch in their face, neck, arm, or leg muscles. Participants will do tasks before and after TUS.

Some visits will include functional magnetic resonance imaging (fMRI) scans. fMRI uses magnet and radio waves to capture images of the activity inside the brain. Participants will lie on a table that slides into a tube. They will perform tasks inside the scanner.

Detailed Description

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Study Description:

Primary olfactory cortex has been implicated in a variety of odorguided behaviors in both animal models and humans. However, direct causal evidence for the functional role of different primary olfactory regions in humans is currently missing, due to the lack of non-invasive neuromodulation methods that can effectively target deep brain regions. With the recent advancement of transcranial focused ultrasound stimulation (TUS), this challenge can now be overcome. The goal of this protocol is to use TUS to temporarily modulate activity in primary olfactory cortex and thereby assess its contribution to olfactory function.

Objectives:

The primary objective is to determine the causal contribution of primary olfactory brain regions to olfactory functions. The secondary objective is to 1) evaluate the neural mechanisms by which these brain regions support these olfactory functions, and 2) assess the safety profile of TUS.

Endpoints:

The primary endpoint is to determine if TUS of olfactory cortices alters olfactory function. The secondary endpoints are to 1) evaluate whether TUS changes neural activity in olfactory regions and 2) assess the degree to which TUS elicits unwanted side effects in healthy adult participants.

Conditions

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Normal Physiology

Keywords

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Behavioral Tasks Decision Making fMRI TUS

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

SINGLE

Participants

Study Groups

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Experiment 1

TUS targeting PirC or sham TUS before olfactory perceptual decision making task

Group Type EXPERIMENTAL

TUS PirC

Intervention Type DEVICE

120 seconds of 100 Hz low-intensity transcranial ultrasound stimulation targeting piriform cortex

TUS Sham

Intervention Type DEVICE

120 seconds of 100 Hz low-intensity transcranial ultrasound stimulation targeting white matter

Experiment 2

TUS targeting amygdala or sham TUS before olfactory conditioning task

Group Type EXPERIMENTAL

TUS Amy

Intervention Type DEVICE

120 seconds of 100 Hz low-intensity transcranial ultrasound stimulation targeting amygdala

TUS Sham

Intervention Type DEVICE

120 seconds of 100 Hz low-intensity transcranial ultrasound stimulation targeting white matter

Interventions

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TUS PirC

120 seconds of 100 Hz low-intensity transcranial ultrasound stimulation targeting piriform cortex

Intervention Type DEVICE

TUS Amy

120 seconds of 100 Hz low-intensity transcranial ultrasound stimulation targeting amygdala

Intervention Type DEVICE

TUS Sham

120 seconds of 100 Hz low-intensity transcranial ultrasound stimulation targeting white matter

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

In order to be eligible to participate in this study, an individual must meet the following criteria:

1. Willingness to comply with all study procedures and availability for the duration of the study.
2. Aged 18-45 years old. Justification: Many neural processes change with age, and these changes could introduce unwanted variability in the measured signals.
3. In good general health based on the assessment of the Medical Advisory Investigator (MAI).
4. Agreement to adhere to Lifestyle Considerations throughout study duration. Consent signature will be documentation of meeting this criterion.
5. Right-handed.

Exclusion Criteria

Individuals who meet any of the following criteria will be excluded from participation:

1. Any neurological disorder that would increase seizure risk from TUS such as stroke, brain lesions, previous neurosurgery, epilepsy, any history of seizure or fainting episode of unknown cause, frequent severe headache, or head trauma resulting in loss of consciousness, lasting over 30 minutes or with sequela lasting longer than one month. The MAI will also retain discretion to exclude based on a history of a neurological illness or trauma that may compromise safety or data integrity.
2. Predisposition to seizures (e.g., first-degree family history of potentially hereditary epilepsy, etc.).
3. Current use (any use in the past week, daily use for more than one week within past 3 months) of any investigational drug or of any medications with psychotropic (e.g., benzodiazepines, etc.), or anti or pro-convulsive action. This will be determined at the discretion of the MAI.
4. Unable to undergo MRI, or TUS due to certain metallic or magnetic devices or implants in the body, claustrophobia, or other reasons.
5. History of noise-induced hearing loss or tinnitus.
6. Recent history (within past 12 months) of learning disability, major DSM-5 psychiatric disorder including major affective disorder, ADHD, obsessivecompulsive disorder, schizophrenia, or PTSD. This will be determined at the discretion of the MAI.
7. Pattern of alcohol and drug use in the past 12 months that is indicative of harmful use, loss of control over use, or physical dependence.
8. Daily nicotine, alcohol, or drug use (excluding caffeine) for at least 4 continuous weeks within the past 12 months.
9. Participation in any neuromodulation (e.g., TMS, TUS, tDCS, tACS, etc.) session (excluding the current protocol) in the past two weeks.
10. History of anaphylaxis, e.g., due to severe asthma, and/or food and non-food allergies (e.g., latex, detergents, soap, etc.).
11. History of significant chronic obstructive pulmonary disease (COPD) as increased levels of carbon dioxide may increase the susceptibility to cavitation and related tissue damage.
12. Uncorrected impairments in visual acuity severe enough to affect task participation.
13. Non-English speaking. Justification: Data integrity of some of the behavioral tasks used in this study would be compromised as they have only been validated in English. Most importantly, ongoing communication regarding safety procedures is necessary when participants are undergoing TUS and MRI procedures. The inability to effectively communicate TUS and MRI safety procedures in a language other than English could compromise the safety of non-English speaking participants.
14. Serious skin disease or serious skin allergy on the hands or head. Justification: Gel will be applied to the skin on both head and hands, for transducer coupling and electrode placement, respectively. Allergic or adverse reactions to such treatment need to be ruled out.
15. Hyposmia, i.e., decreased sense of smell, or anosmia, i.e., loss of smell (Sniffin' Sticks Olfactory Identification Score \< 10). Justification: The ability to smell is paramount to task performance.
16. Pregnancy. Justification: It is unknown whether MRI and pose risks to fetuses.
17. Any other condition that in the judgment of the investigators is incompatible with participation.
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Institute on Drug Abuse (NIDA)

NIH

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Thorsten Kahnt, Ph.D.

Role: PRINCIPAL_INVESTIGATOR

National Institute on Drug Abuse (NIDA)

Locations

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National Institute on Drug Abuse

Baltimore, Maryland, United States

Site Status

Countries

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United States

Central Contacts

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NIDA IRP Screening Team

Role: CONTACT

Phone: (800) 535-8254

Email: [email protected]

Thorsten Kahnt, Ph.D.

Role: CONTACT

Phone: (667) 312-5175

Email: [email protected]

Facility Contacts

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Thorsten Kahnt

Role: primary

Other Identifiers

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002358-DA

Identifier Type: -

Identifier Source: secondary_id

10002358

Identifier Type: -

Identifier Source: org_study_id