Effects of Pulse Consumption, Gut Microbiome, and Appetite in Healthy Participants

NCT ID: NCT07043712

Last Updated: 2025-06-29

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

ACTIVE_NOT_RECRUITING

Clinical Phase

NA

Total Enrollment

44 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-02-01

Study Completion Date

2025-12-31

Brief Summary

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The purpose of the study is to investigate the effects of pulse consumption versus no pulse consumption on the gut microbiome, meal satiety, and short-chain fatty acid metabolomics.

Detailed Description

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1. Investigate the acute and chronic effects of pulse consumption on the microbiome and plasma SCFA metabolomics in overweight subjects fed a high pulse (n=22) diet versus a no pulse containing diet (n=22).
2. Furthermore, we will quantitate the rate of production of plasma SCFA, markers of satiety (PYY, GLP-1 and ghrelin), blood lipids, and carbohydrate (CHO) metabolism (glucose levels and oxidation) during a standardized meal to determine the effects of consuming pulses on appetite and satiety after acute and chronic dietary consumption.
3. We will conduct subjective appetite assessments to investigate appetite control over the short- and long-term.

Conditions

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Microbial Colonization Metabolic Syndrome

Keywords

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overweight satiety SCFA pulses

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Participants are randomly assigned to either the high pulse diet or no pulse diet.
Primary Study Purpose

OTHER

Blinding Strategy

SINGLE

Outcome Assessors

Study Groups

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High pulse consumption

Subjects are provided a high pulse diet for 4-weeks. Tests include: blood biochemistries, a meal tolerance test, body composition measured via DEXA, surveys, fecal sample

Group Type EXPERIMENTAL

High pulse consumption

Intervention Type DIETARY_SUPPLEMENT

Subjects are provided a high pulse diet for 4 weeks.collection, and anthropometrics measured.

No pulse consumption

Subjects are provided a diet that has no pulses for 4-weeks. Tests include: blood biochemistries, a meal tolerance test, body composition measured via DEXA, surveys, fecal sample

Group Type NO_INTERVENTION

No interventions assigned to this group

Interventions

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High pulse consumption

Subjects are provided a high pulse diet for 4 weeks.collection, and anthropometrics measured.

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* Men and women (premenopausal only)
* Age 20-55y (we will attempt to start baseline testing at the same time of the menstrual cycle (luteal) for the women
* BMI ≥25 or ≤40 kg/m2 (most at risk for metabolic abnormalities and will benefit from a pulse intervention-2/3 of Americans are overweight or obese)
* Weight stable (no fluctuations in body weight of greater than 4 kg in the last 3 months)
* Willing to consume a research diet
* Willing to provide blood and fecal samples
* Sedentary to low active physical activity status (less than 7200 steps per day)
* Stably treated with statin drugs, anti-hypertensives, and anti-depressants. These are acceptable as long as the drug category does not alter appetite, body weight, or the microbiome (if known)
* Pre-diabetes acceptable (glucose \<125 mg/dL or HbA1c \< 6.5%)
* At least one characteristic of the metabolic syndrome (but not diabetic)

1. A large waistline: 35 inches or more for women 40 inches or more for men
2. High triglycerides: 150 mg/dL or higher
3. Low HDLc level: \<50 mg/dL for women \<40 mg/dL for men
4. High blood pressure ≥130/85 mmHg
5. Fasting blood sugar ≥100 mg/dL - Pre-diabetes acceptable (glucose \<125 mg/dL or HbA1c \<6.5%) -

Exclusion Criteria

* Pregnant or lactating
* Postmenopausal (evidence suggests an interplay between the gut microbiome)
* BMI of \<25 or \>40 kg/m2
* Use of medications that affect the gut microbiome (e.g. antibiotics)
* Taking medications known to affect appetite (e.g., phentermine) or gastrointestinal function (e.g., metformin)
* On a special diet or undergoing weight loss, vegetarian, or other restricted dietary patterns
* Ad libitum intake of fiber above 25g/day (mean intake in the US population is 17g/day) and \< 10g/d
* History of disease (example colon cancer, HIV, cardiovascular disease, psychiatric disorders, etc.)
* Use of tobacco products
Minimum Eligible Age

20 Years

Maximum Eligible Age

55 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of Missouri-Columbia

OTHER

Sponsor Role lead

Responsible Party

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Katherene Anguah

Associate Professor, Nutrition and Exercise Physiology

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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University of Missouri

Columbia, Missouri, United States

Site Status

Countries

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United States

Other Identifiers

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2066363

Identifier Type: -

Identifier Source: org_study_id