The Ameliorative Effect of C-Kit (+) Hepatic Endothelial Cells With Mertk Deficiency on Nonalcoholic Steatohepatitis

NCT ID: NCT06913699

Last Updated: 2025-04-08

Basic Information

• Recruitment Status: ACTIVE_NOT_RECRUITING
• Total Enrollment: 6 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2024-04-01
• Study Completion Date: 2026-05-31

Brief Summary

The prevalence of non-alcoholic fatty liver disease (NAFLD) has been steadily increasing, with 10-20% of affected individuals progressing to non-alcoholic steatohepatitis (NASH). NASH is pathologically characterized by hepatic inflammation, steatosis, and hepatocyte injury. Furthermore, this condition carries a significant risk of progression to advanced hepatic fibrosis (pathological Scheuer fibrosis stage F≥3), cirrhosis, and even hepatocellular carcinoma (HCC). In recent years, NASH has emerged as the leading contributor to the growing burden of cirrhosis worldwide, representing a major public health challenge. Despite the high incidence and clinical severity of NAFLD, there are currently no FDA-approved therapeutic agents for its management. Therefore, elucidating the molecular mechanisms underlying NAFLD-associated NASH progression is critical for developing targeted pharmacological interventions capable of preventing, ameliorating, or potentially reversing disease progression.

Detailed Description

I. Study Background Non-alcoholic fatty liver disease (NAFLD) has become one of the most prevalent liver disorders globally. Despite its high incidence and severity, no effective therapeutic agents currently exist. Existing treatments focus on symptomatic relief, such as hepatoprotection and enzyme reduction, rather than addressing the underlying pathogenesis. Therefore, elucidating the mechanisms driving the progression of NAFLD-related non-alcoholic steatohepatitis (NASH) and hepatic fibrosis is critical for developing preventive, ameliorative, or even curative strategies, holding significant strategic importance.

1. Role of LSECs in NAFLD Pathogenesis:

Liver sinusoidal endothelial cells (LSECs), the primary components of the hepatic sinusoidal endothelium, form a physical barrier regulating substance exchange between the hepatic parenchyma and circulation. As highly specialized endothelial cells (ECs) and the most abundant non-parenchymal cells (NPCs) in the liver, LSECs play pivotal roles in hepatic pathophysiology. Targeting LSEC function represents a promising approach to mitigate NAFLD progression and complications.

2. Heterogeneous Transcriptomic Features of LSEC Subpopulations in NAFLD:

Single-cell RNA sequencing (scRNA-seq) enables genome-wide amplification and sequencing at the single-cell level. In the liver, hepatocytes (HCs) and NPCs exhibit marked transcriptomic heterogeneity, underpinning metabolic and functional zonation. This highlights the potential of single-cell analysis in understanding liver diseases. However, scRNA-seq studies on LSECs in NAFLD remain unexplored.

3. Hypothesis: C-Kit(+)/Gas6(-)-LSEC Subpopulation Exacerbates NAFLD via Gas6/Mertk-Mediated Regulation of PINK1 Mitophagy:

Bone marrow-derived endothelial progenitor cells (EPCs), marked by C-Kit (CD117), may express C-Kit in ECs under pathological conditions. Gas6, a ligand for TAM receptors (Tyro3, Axl, Mertk), activates downstream signaling cascades regulating cell migration, inflammation, survival, and mitophagy. In NAFLD, Gas6/Mertk exhibits cell-specific roles : Anti-inflammatory in hepatic macrophages; Pro-fibrotic in hepatic stellate cells (HSCs); Protective in hepatocytes (HCs). However, the role of Gas6/Mertk in LSECs during NAFLD remains unknown. Mitochondrial dysfunction is a hallmark of NAFLD, and impaired mitophagy (e.g., PINK1 deficiency) exacerbates hepatic inflammation, steatosis, and fibrosis. Emerging evidence suggests Gas6/Mertk enhances mitophagy to preserve mitochondrial function. For instance, Mertk-/- mice exhibit cardiomyocyte mitochondrial dysfunction, and Gas6 promotes mitophagy.

Our preliminary scRNA-seq data from HFD/ND mice identified a unique C-Kit(+)/Gas6(-)-LSEC subpopulation. These LSECs induced mitophagy impairment in co-cultured HCs/HSCs, manifesting as mitochondrial senescence, oxidative stress, HSC activation, HC lipid deposition, and inflammation. Investigating how this subpopulation drives NAFLD progression will unveil novel pathophysiological mechanisms and therapeutic targets, forming the basis of this study.

II. Study Objectives To elucidate the expression levels of C-Kit and Gas6/Mertk ligand-receptor pairs in liver tissues of NAFLD patients.

III. Study Procedures

(1) Inclusion and Exclusion Criteria

1. Inclusion Criteria: NAFLD diagnosis per Guidelines for the Prevention and Treatment of Non-Alcoholic Fatty Liver Disease (2018 Edition). Age 18-65 years.

2. Exclusion Criteria: Cirrhosis (compensated or decompensated), hepatocellular carcinoma, active viral hepatitis (HBV, HCV), primary biliary cholangitis, primary sclerosing cholangitis, recent history of illicit drug use, alcohol intake≥20 g/day for women and ≥30 g/day for men, current pregnancy or breastfeeding, severe comorbidities.

(2) Study Design

1. Liver Biopsy and Tissue Collection:

Ultrasound-guided liver biopsy in 6 NAFLD patients. Histopathological evaluation (HE, Masson staining) for NASH and fibrosis (NAS and Scheuer scoring). Clinical data collection and liver tissue storage (-80°C).

2. Expression Analysis of C-Kit and Mertk:

Immunofluorescence co-staining of liver tissues. 3. Statistical Analysis: Compare C-Kit and Mertk expression levels in mild and severe NAFLD patients.

(3) Enrollment and Workflow

1. Baseline Data Collection:

①Demographic and clinical parameters: name, sex, age, ethnicity, birthdate, height, weight, disease onset, contact information.

② Laboratory tests: CBC, ALT, AST, TBIL, TG, cholesterol, glucose, total protein, albumin.

③Clinical assessments: blood pressure, BMI, abdominal ultrasound, FibroScan.

2. Biopsy Protocol:

• Ultrasound-guided percutaneous liver biopsy within 1 month of enrollment. ②Tissue specimen requirements: ≥3 mm diameter, 5-8 mm length. ③Storage: -80°C for C-Kit and Mertk gene analysis.

IV. Expected Outcomes Clarify the regulatory relationship between C-Kit (+) LSECs and the Gas6-Mertk signaling pathway in NAFLD patient liver tissues.

Conditions

Non-alcoholic Fatty Liver Disease (NAFLD)

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: CROSS_SECTIONAL

Interventions

Ultrasonund-guided percutaneous liver biopsy

All patients completed blood routine, coagulation function and liver biochemical indexes before surgery, and informed the patients and their families about the purpose and risks of surgery, and signed an informed consent. During the procedure, the patient was placed in the left lateral decubitus position. After locating by ultrasound and routine disinfection, 0.5% lidocaine was used for local anesthesia. Under the guidance of ultrasound, a 16G disposable biopsy needle (SC1620) was inserted into the liver parenchyma, and the biopsy gun was started and fired for sampling. The removed liver tissue was fixed in 4% formaldehyde solution and sent to the pathology department. The patient's puncture site was disinfected and covered with sterile dressing, and the abdominal band was bandaged. The patients were instructed to stay in bed for 24 hours after operation, closely observe whether there was pain and bleeding after operation, and monitor electrocardiogram for 24 hours.

Intervention Type: PROCEDURE

Eligibility Criteria

Inclusion Criteria

• Clinical diagnosis of Non-Alcoholic Fatty Liver Disease
• Age 18-65 years

Exclusion Criteria

• Cirrhosis (compensated or decompensated)
• Hepatocellular carcinoma
• Active viral hepatitis (HBV, HCV)
• Primary biliary cholangitis
• Primary sclerosing cholangitis
• Recent history of illicit drug use
• Alcohol intake≥20 g/day for women and ≥30 g/day for men
• Current pregnancy or breastfeeding
• Severe comorbidities.

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 65 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

National Natural Science Foundation of China

OTHER_GOV

Sponsor Role: collaborator

Shanghai East Hospital

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Locations

Shanghai East Hospital (East Hospital Affiliated To Tongji University)

Shanghai, Shanghai Municipality, China

Countries

China

Other Identifiers

Approval No.2024YS-050

Identifier Type: -

Identifier Source: org_study_id