The Impact of Gestational Diabetes Mellitus on Cerebral Blood Flow and Cerebrovascular Function After Pregnancy

NCT ID: NCT06911372

Last Updated: 2025-12-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE1

Total Enrollment

84 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-08-22

Study Completion Date

2028-04-15

Brief Summary

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Gestational Diabetes Mellitus (GDM) currently affects approximately 14% of all pregnancies worldwide. Importantly, the health-related consequences of GDM extend well beyond pregnancy, such that women with a history of GDM have a 40% increased risk of cerebrovascular diseases and a 67% increased risk of dementia, compared to women with a history of uncomplicated pregnancy. Women with a history of GDM have impaired skin microvascular function, compared with women with a history of uncomplicated pregnancy. Therefore, it's likely that GDM results in impaired brain blood vessel function, yet there is little-to-no information regarding the effects of GDM on brain blood vessel health and function after pregnancy.

Therefore, the purpose of the study is to evaluate the effects of GDM on brain blood flow and brain blood vessel function in healthy women with either a history of GDM or uncomplicated pregnancy.

In this study, the investigators will use two different types of ultrasound to non-invasively measure brain blood flow. Brain blood vessel function will be evaluated by examining the brain blood flow responses to increases in carbon dioxide (the increases in carbon dioxide are similar to what is experienced during a breath hold). Additionally, the investigators will compare the brain blood flow results to skin microvascular function to explore potential mechanisms behind possible impairments in brain blood vessel function. Skin microvascular function will be assessed using a minimally invasive technique (intradermal microdialysis for the local delivery of pharmaceutical agents) on dime sized areas of the forearm. Finally, for screening purposes and to further explore potential mechanisms behind any potential impairments in brain blood vessel function, the investigators will perform blood draws to determine the metabolic health of the participants and to analyze for substances that influence blood vessel function.

Detailed Description

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Conditions

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Gestational Diabetes Mellitus (GDM) Postpartum Women Uncomplicated Pregnancy

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Vascular Tests

All women will complete the same protocol, regardless of pregnancy history.

Group Type EXPERIMENTAL

Intradermal Microdialysis

Intervention Type DRUG

A total of three microdialysis sites will be perfused with ringer's solution, L-NAME, or ketorolac (one drug per site). After a baseline period, all three sites will undergo tests of blood vessel function including an acetylcholine dose-response protocol and a test of maximal skin blood flow with sodium nitroprusside.

Brain Blood Flow Tests

Intervention Type OTHER

Participants will complete CO2 breathing procedures to evaluate brain blood vessel health.

Local Heating

Intervention Type OTHER

A dime-sized heater will be placed on the skin to evaluate cutaneous microvascular function

Interventions

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Intradermal Microdialysis

A total of three microdialysis sites will be perfused with ringer's solution, L-NAME, or ketorolac (one drug per site). After a baseline period, all three sites will undergo tests of blood vessel function including an acetylcholine dose-response protocol and a test of maximal skin blood flow with sodium nitroprusside.

Intervention Type DRUG

Brain Blood Flow Tests

Participants will complete CO2 breathing procedures to evaluate brain blood vessel health.

Intervention Type OTHER

Local Heating

A dime-sized heater will be placed on the skin to evaluate cutaneous microvascular function

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Post-partum women
* 18 years or older
* Delivered within 5 years from the study visit
* History of gestational diabetes diagnosed by an obstetrician and confirmed according to the American College of Obstetricians and Gynecologists criteria for gestational diabetes OR history of uncomplicated pregnancy (defined as no history of gestational diabetes, hypertensive pregnancy, or other gestational disorder).

Exclusion Criteria

* Skin diseases
* Current tobacco use
* Diagnosed or suspected hepatic or metabolic disease including diabetes
* Diagnosed with depression or other mood-related disorders, asthma, chronic obstructive pulmonary disease, cystic fibrosis, or pulmonary fibrosis
* Use of prescribed NSAIDs, statins or other cholesterol-lowering medication, antihypertensive medication, carbonic anhydrase inhibitors, corticosteroids, thyroid medications, antidepressants or mood stabilizers, diuretics, phenothiazines, or benzodiazepines
* History of preeclampsia or gestational hypertension,
* History or family history of panic disorder,
* Currently pregnant
* Body mass index \<18.5 kg/m2,
* Allergy to materials used during the experiment (e.g. latex), known allergies to study drugs.
* History of heavy alcohol use/binge drinking,
* Have planned procedures with radiological contrast,
* Have a major dental procedure/surgery coming up, such as a dental extraction
* Anatomy of the middle cerebral artery or internal carotid artery that prevents adequate ultrasonography and/or and data collection
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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Anna Stanhewicz, PhD

OTHER

Sponsor Role lead

Responsible Party

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Anna Stanhewicz, PhD

Assistant Professor

Responsibility Role SPONSOR_INVESTIGATOR

Locations

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Iowa Bioscience Innovation Facility

Iowa City, Iowa, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Anna Reid-Stanhewicz, PHD

Role: CONTACT

319-467-1732

Facility Contacts

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Anna Stanhewicz, P.h.D

Role: primary

3194671732

Claire Goebel, B.S.

Role: backup

5158359904

Other Identifiers

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202410260

Identifier Type: -

Identifier Source: org_study_id