Blood Tuberculosis DNA Levels to Monitor Tuberculosis Treatment

NCT ID: NCT06845618

Last Updated: 2026-01-08

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Total Enrollment

140 participants

Study Classification

OBSERVATIONAL

Study Start Date

2026-02-01

Study Completion Date

2027-09-30

Brief Summary

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Tuberculosis (TB) is a leading infectious cause of death worldwide. Current strategies for monitoring TB treatment response are culture dependent and insensitive. New methods of assessing treatment response in vivo could inform new drug development and other treatment strategies. Cell-free DNA (cfDNA) - small circulating fragments of DNA - is widely used in maternofetal medicine and oncology for diagnosis and assessment of treatment response. This study aims to investigate whether pathogen derived Mycobacterium tuberculosis-specific cfDNA (Mtb-cfDNA) can be used to monitor TB treatment response.

This feasibility study will take place at Mae RaMat TB Center in Thailand and includes two study groups:

1. Assay Development and Validation
2. Longitudinal Assessment of Mtb-cfDNA levels

Detailed Description

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This study is funded by the Wellcome Trust; grant reference number: 223099/Z/21/Z

Conditions

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Mycobacterium Tuberculosis Tuberculosis, Pulmonary Tuberculosis, Extra-Pulmonary

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Group 1: Assay development and validation

Twenty participants with a new diagnosis of tuberculosis will have venous blood collected prior to treatment initiation. Twenty participants without clinical evidence of tuberculosis infection will be recruited from the local community as a control during assay validation. This group of the study participants will be assessed at day zero only.

No interventions assigned to this group

Group 2: Longitudinal Assessment

In this group, tuberculosis participants (n= 120) will have longitudinal sampling performed from diagnosis to the end of treatment. This will establish the feasibility of dynamic Mtb-cfDNA measurements for the assessment of tuberculosis treatment response.

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

Participants with a new diagnosis of tuberculosis

* Aged ≥ 18 years old
* Newly microbiologically confirmed (culture or nucleic acid amplification test) diagnosis of Mycobacterium tuberculosis (Mtb.) infection (of any site)
* Has not yet commenced antituberculosis therapy
* Able to understand study procedures and requirements and is able to give informed consent

For healthy volunteers:

* Aged ≥ 18 years old
* Healthy as judged by a responsible physician
* Able to understand study procedures and requirements and is able to give informed consent

Exclusion Criteria

Participants with a new diagnosis of tuberculosis

* Exposure to antituberculosis treatment in the last 8 weeks (or Mycobacterium tuberculosis (Mtb.) active fluoroquinolone)
* Known history of underlying malignancy
* Pregnancy
* Transfusion dependent anaemia

For healthy volunteers:

* History of tuberculosis infection or latent tuberculosis infection
* Household, or other close contact, of a person living with tuberculosis disease
* Chest radiograph (CXR) changes suggestive of pulmonary tuberculosis
* Presence of symptoms which would otherwise indicate screening for tuberculosis (cough \> 2 weeks duration, fever, weight loss, night sweats)
* Other major medical comorbidity
* Pregnancy
* Known malignancy
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of Oxford

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Timothy Seers, Dr

Role: PRINCIPAL_INVESTIGATOR

Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University.

Locations

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Shoklo Malaria Research Unit (SMRU)

Tak, , Thailand

Site Status

Countries

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Thailand

Central Contacts

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Htet Ko Ko Aung, Dr

Role: CONTACT

055 581 135 ext. 109

François Nosten, Professor

Role: CONTACT

055 532026

Facility Contacts

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Htet Ko Ko Aung, Dr

Role: primary

055 581 135 ext. 109

François Nosten, Professor

Role: backup

055 532026

Other Identifiers

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MYC24003

Identifier Type: -

Identifier Source: org_study_id

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