Post Extraction Alterations Following Ridge Preservation In The Esthetic Zone Using Partially Demineralized Autogenous Dentine Graft Versus Xenograft

NCT ID: NCT06552793

Last Updated: 2024-08-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

23 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-05-01

Study Completion Date

2025-02-01

Brief Summary

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the aim of this study is to establish if there are clinical, radio-graphical and histomorphometrical differences between using Partially Demineralized Dentin graft versus Xenograft in the esthetic zone. as a clinical application in alveolar bone regeneration procedures related to implant dentistry, including socket preservation, alveolar ridge augmentation, after tooth extraction in the esthetic zone. After extraction of non-restorable teeth in the esthetic zone, will ridge preservation with application of partially demineralized autogenous dentin graft produce less hard tissue changes compared to xenograft?

The initial therapy consists of periodontal treatment (phase I therapy) including supragingival scaling, subgingival debridement if needed, adjustment of faulty restoration and polishing. The mechanical plaque control instructions for each patient include brushing and interdental cleaning techniques. Flapless and atraumatic tooth extraction will be initiated, Then the sockets will be carefully packed with the allocated graft material that are shaped to match the individual size and contours of each socket. Once the grafts are properly adapted to the sockets, they will be covered with collagen membrane. The membrane will be shaped to extend 2-3 mm beyond the margins of the extraction socket and positioned just beneath the marginal mucosa. To ensure its stability, a cross-suture will be performed, securing the membrane in place.

Intervention group: The socket will be filled with partially demineralized autogenous dentin graft Control group: The socket will be filled with xenograft . For both groups, All the subjects will be evaluated at pre-surgical, baseline and 6 months post surgical months for clinical parameters and baseline( immediate post-surgical and 6 months post-surgical . Outcomes: Change in radiographic horizontal ridge width at 1mm below the most coronal aspect of the crest , Change in radiographic horizontal ridge width at 3 and 5mm below the most coronal aspect of the crest,Change in radiographic buccal ridge height , Change in radiographic buccal ridge height ,Change in radiographic Palatal ridge height,Percentage of new vital bone formation,Percentage of residual bone graft,Implant Primary Stability.

Detailed Description: This study aims to evaluate clinical, radiographic and histomorphometrica

Detailed Description

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The study aims to radiographically evaluate the dimentionsal changes of hard tissue after application of partailly deminerlized dentin graft verus xenograft in the esthatic zone .

Research Procedure in brief:

The study is to be conducted in the Oral Medicine and Periodontology department, Faculty of Dentistry- Cairo University, Egypt.Patients are to be selected from the outpatient clinic of the department of Oral Medicine and Periodontology, clinic of the department of Oral surgery and clinic of the department of Endodontics -Cairo University. The initial therapy consists of periodontal treatment (phase I therapy) including supragingival scaling, subgingival debridement if needed, adjustment of faulty restoration and polishing. The mechanical plaque control instructions for each patient include brushing and interdental cleaning techniques. surgical interventions: Atraumatic extraction.The patient will rinse their mouth with 0.12% Chlorhexidine Administration of 4% articaine hydrochloride with 1:100,000 epinephrine will be as local anesthetic agent.Flapless and atraumatic tooth extraction will be initiated, using 15C blade intrasulcular incision will be performed.A periotome will be utilized to sever the periodontal ligament (PDL) fibers, followed by extraction witha straight elevator and extraction forceps.

In the test group:

The autogenous partially demineralized dentin graft will be prepared as follows:

A high-speed fine finishing stone and saline irrigation will be used to clean the tooth and remove any decay, restoration, or foreign materials. Tooth will be rinsed twice in phosphate buffered saline. The tooth will be dried using air and ground with the Tooth Transformer device following the manufacturer's protocol. Dentin particles will be obtained with a dimension of 400 - 800 μm .

Preparation of the autogenous partially demineralized dentin matrix

* The extracted tooth will be cleaned with a diamond bur under abundant irrigation with physiological water. All filling materials (gutta-percha, composite, luting cements, etc.) will be removed with the outmost care and under magnification.
* Subsequently, the tooth will be cut into fragments (5 × 5 mm) and inserted into the milling device . According to the manufacturer, a disposable box containing disposable liquid solutions will be inserted into the device to ensure the demineralization of the graft with 0.1 M hydrochloric acid, 10% hydrogen peroxide, and demineralized water as a wash.
* The decontamination of the granules will take place through UVA rays and ultrasounds, with temperature variations always lower than 43 ◦C to avoid damage to proteins.
* After 25 min, particle graft biomaterials will be obtained. The particles size will range from 815 µm with peaks up to 1110 µm.
* All bone defects will be filled with the graft obtained from the TT grinder.
* Device for Tooth Processing (The Tooth Transformer TT):

Part 1: Tooth Cleaning After extraction, the tooth must be cleaned of tooth decay, tartar, soft tissue debris, fillings, cement, and prosthetic components. For the TT®, which calls for cutting the tooth into sections for the trituration stage .

Part 2: Tooth Grinding The TT® is equipped with a multipurpose sterilizable system that operates at low speed, which makes it possible to avoid the loss of tooth substance during pulverisation, despite the drawback of not being able to enter an entire tooth into the shredder .

Part 3: Treatment by Device The shredder is closed and inserted once the tooth has been placed. When the door is closed and the button is pressed, a liquid cartridge and a cylinder with a granulate collection cup (maker) are placed into the apparatus in their respective housings, the cartridge is activated by punching, and the procedure starts .

In control group:

Xenograft will be prepared in a separate room to keep patients blinded, where the allocated material will be administered 15 minutes after tooth extraction. A xenograft will be implanted in the alveolus.

Alveolar ridge preservation:

Following a thorough cleaning procedure,

Biopsy collection:

After 6 months healing a local anesthesia will be given, a full thickness mucoperiosteal flap will be detached in the augmented areas and a bone biopsy will be collected using a 4 mm trephine bur.

Implant placement:

After 6 months healing a local anesthesia will be given, a full thickness mucoperiosteal flap will be detached in the augmented areas an implant placement with sequences drill will be done.

Postoperative Care and follow up:

Participants will be given instructions to avoid any trauma to the operative site, refrain from interfering with the sutures, and to avoid consuming hot food or engaging in vigorous rinsing.

They will be encouraged to continue tooth brushing for the other parts of their dentition, while gentle tooth brushing for the operative site will be advised to be resumed after a two-week period.

Participants will be prescribed either Amoxicillin 500 mg (Misr Co. for Pharmaceutical Industries, Egypt) three times daily for seven days, or doxycycline 100 mg (Doxymycin, Nile Co. for Pharmaceuticals and Chemical Industries, Egypt) twice daily if they are sensitive to penicillin. In case of severe pain, Ibuprofen 600 mg (Brufen, Kahira Pharmaceuticals, Egypt) will be prescribed. To facilitate gentle rinsing, participants will be instructed to use a 0.12% Chlorhexidine mouthwash twice daily for a duration of two weeks .

The sutures will be removed two weeks after the surgery. On the first day postoperatively, a baseline cone-beam computed tomography (CBCT) scan will be conducted. A final follow-up visit and CBCT scan will be scheduled for six months postoperatively.

Conditions

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Socket Preservation

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

A randiomized clinical trial
Primary Study Purpose

TREATMENT

Blinding Strategy

TRIPLE

Participants Investigators Outcome Assessors

Study Groups

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Autogenous partially demineralized dentin graft

Following atraumatic tooth extraction, curettes will be used to remove granulation tissues, and the socket will be irrigated with sterile normal saline.Then the socket will be filled with Autogenous partially demineralized dentin graft (that will be prepared using tooth transformer device) followed by placement of a collagen membrane to cover the socket(The membrane will be shaped to extend 2-3 mm beyond the margins of the extraction socket and positioned just beneath the marginal mucosa) . Suturing technique will be a criss cross horizontal mattress to ensure that most of the grafting material is covered.

Group Type EXPERIMENTAL

autogenous partially demineralized dentin graft

Intervention Type BIOLOGICAL

tooth cleaning : form decay, tartar, soft tissue debris, fillings, cement then cut into fragments (5×5 mm) and inserted into the milling device (Tooth Transformer, Milan, Italy). A disposable box containing disposable liquid solutions will be inserted into the device to ensure the demineralization of the graft with 0.1 M hydrochloric acid, 10% hydrogen peroxide, and demineralized water as a wash.The decontamination of the granules will take place through UVA rays and ultrasounds, with temperature variations always lower than 43 ◦C to avoid damage to proteins. After 25 min, particle graft biomaterials will be obtained. The particles size will range from 815 µm with peaks up to 1110 µm.

Xenograft

Following atraumatic tooth extraction, curettes will be used to remove granulation tissues, and the socket will be irrigated with sterile normal saline.Then the socket will be filled with xenograft graft followed by placement of a collagen membrane to cover the socket.( The membrane will be shaped to extend 2-3 mm beyond the margins of the extraction socket and positioned just beneath the marginal mucosa) Suturing technique will be a criss cross horizontal mattress to ensure that most of the grafting material is covered.

Group Type ACTIVE_COMPARATOR

Xenograft

Intervention Type BIOLOGICAL

Cortico-cancallous Bovine powder

Interventions

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autogenous partially demineralized dentin graft

tooth cleaning : form decay, tartar, soft tissue debris, fillings, cement then cut into fragments (5×5 mm) and inserted into the milling device (Tooth Transformer, Milan, Italy). A disposable box containing disposable liquid solutions will be inserted into the device to ensure the demineralization of the graft with 0.1 M hydrochloric acid, 10% hydrogen peroxide, and demineralized water as a wash.The decontamination of the granules will take place through UVA rays and ultrasounds, with temperature variations always lower than 43 ◦C to avoid damage to proteins. After 25 min, particle graft biomaterials will be obtained. The particles size will range from 815 µm with peaks up to 1110 µm.

Intervention Type BIOLOGICAL

Xenograft

Cortico-cancallous Bovine powder

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* Patients with non-restorable teeth and Type II extraction socket in the esthetic zone indicated for extraction for periodontal, carious or traumatic reasons.
* Healthy patients with adequate oral hygiene (bleeding on probing ≤20%; Plaque index ≤20%).
* Adult patients above 18 years old.
* Patients accept 6-months follow-up period and provide an informed consent.

Exclusion Criteria

* Heavy smokers (more than 10 cigarettes per day or an electronic cigarette dose of \>6 mg/ml of nicotine).
* Patients reporting systemic conditions that may compromise healing or bone metabolism (eg: diabetes).
* Patients with poor oral hygiene (bleeding on probing \>20%; Plaque index \>20%)
* The presence of acute periapical infection.
* The presence of severe periodontal destruction.
Minimum Eligible Age

18 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Cairo University

OTHER

Sponsor Role lead

Responsible Party

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Riham Ahmad Alshiek Hani Alkurdee

principle investigator Riham Ahmad Master degree student, periodontology department, faculty of dentistry, Cairo University

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Manal M Hosny, Professor

Role: STUDY_DIRECTOR

Cairo University

Weam A El-Battawy, Ass. Prof.

Role: STUDY_CHAIR

Cairo University

Riham A Alshikh Hani Alkurdee, Bachelor

Role: PRINCIPAL_INVESTIGATOR

Cairo University

Locations

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Faculty of Dentistry Cairo University

Cairo, , Egypt

Site Status RECRUITING

Countries

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Egypt

Central Contacts

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Riham A Alshikh Hani Alkurdee, Bachelor

Role: CONTACT

+201021931950

Weam El-Battawy, Ass. Prof.

Role: CONTACT

Facility Contacts

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Riham A Alshikh Hani Alkurdee, Bachelor

Role: primary

01021931950

Weam A El-Battawy, Ass. Prof.

Role: backup

Other Identifiers

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21524PER6-3-1

Identifier Type: -

Identifier Source: org_study_id

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