QuantifyHER: Quantitative Immunofluorescence and/or RT-qPCR for Measuring HER2 in HER2-low Metastatic Breast Cancer

NCT ID: NCT06551116

Last Updated: 2025-09-05

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

200 participants

Study Classification

OBSERVATIONAL

Study Start Date

2024-10-10

Study Completion Date

2029-09-01

Brief Summary

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This study will assess whether a quantitative, HER2 assay can accurately and reliably discriminate between responders and non-responders among patients with HER2 IHCI+ metastatic breast cancer who are receiving T-Dxd.

Detailed Description

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Conditions

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HER2-positive Metastatic Breast Cancer

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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HER2 Assay

Analysis of HER2 expression via QIF and mRNA assays

CE-10-IVD

Intervention Type DIAGNOSTIC_TEST

Leftover tumor tissue from a routine biopsy will be sent for analysis.

Interventions

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CE-10-IVD

Leftover tumor tissue from a routine biopsy will be sent for analysis.

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Women and men age \> 18 years
* Metastatic breast cancer, histologically- confirmed. Any estrogen receptor (ER) status is allowed. ER status will be determined by local laboratory assessment utilizing ASCO/CAP guidelines.
* Primary and/or metastatic tumor with 1+ level of expression of HER2 by immunohistochemistry as determined by local laboratory assessment utilizing ASCO/CAP guidelines.
* Measurable disease by cross-sectional imaging at the start of treatment. Patients with measurable bone-only disease or active brain metastases are eligible.
* Archival tissue available for biomarker assessment. One specimen should be the most recent metastatic biopsy. If HER2 1+ status was determined on a different specimen (either primary or metastatic tissue), that specimen is also required. Samples obtained from bone metastases that were processed via decalcification methods are not eligible.
* Intention to initiate therapy with T-DXd (Enhertu) at FDA-approved dose and schedule as next line of therapy. If T-DXd was already initiated, patients must be registered within 30 days of initiation.
* Ability to provide informed consent

Exclusion Criteria

* Concurrent Her2-overexpressing metastatic breast cancer (as confirmed by a metastatic biopsy with IHC 3+ or IHC 2+ with FISH amplified as per standard ASCO/CAP guidelines)
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Translational Breast Cancer Research Consortium

OTHER

Sponsor Role collaborator

Danaher Inc.

UNKNOWN

Sponsor Role collaborator

Abramson Cancer Center at Penn Medicine

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Angela DeMichele, MD

Role: PRINCIPAL_INVESTIGATOR

Abramson Cancer Center at Penn Medicine

Locations

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University of California San Francisco Medical Center

San Francisco, California, United States

Site Status RECRUITING

Smilow Cancer Hospital-Derby Care Center

Derby, Connecticut, United States

Site Status RECRUITING

Smilow Cancer Hospital Care Center

Fairfield, Connecticut, United States

Site Status RECRUITING

Smilow Cancer Hospital at Glastonbury

Glastonbury, Connecticut, United States

Site Status RECRUITING

Smilow Cancer Hospital Care Center at Greenwich

Greenwich, Connecticut, United States

Site Status RECRUITING

Smilow Cancer Hospital Care Center

Guilford, Connecticut, United States

Site Status RECRUITING

Smilow Cancer Hospital at Saint Francis

Hartford, Connecticut, United States

Site Status RECRUITING

Yale-New Haven Hospital North Haven Medical Center

New Haven, Connecticut, United States

Site Status RECRUITING

Yale Cancer Center

New Haven, Connecticut, United States

Site Status RECRUITING

Smilow Cancer Hospital Care Center at Long Ridge

Stamford, Connecticut, United States

Site Status RECRUITING

Smilow Cancer Hospital-Torrington Care Center

Torrington, Connecticut, United States

Site Status RECRUITING

Smilow Cancer Hospital Care Center

Trumbull, Connecticut, United States

Site Status RECRUITING

Smilow Cancer Hospital-Waterbury Care Center

Waterbury, Connecticut, United States

Site Status RECRUITING

Smilow Cancer Hospital Care Center - Waterford

Waterford, Connecticut, United States

Site Status RECRUITING

Georgetown University - Lombardi CCC

Washington D.C., District of Columbia, United States

Site Status RECRUITING

MedStar Washington Hospital Center

Washington D.C., District of Columbia, United States

Site Status RECRUITING

Sibley Memorial Hospital

Washington D.C., District of Columbia, United States

Site Status RECRUITING

University of Chicago Medical Center

Chicago, Illinois, United States

Site Status RECRUITING

University of Chicago Comprehensive Cancer Center at Silver Cross

New Lenox, Illinois, United States

Site Status RECRUITING

University of Chicago Medicine-Orland Park

Orland Park, Illinois, United States

Site Status RECRUITING

University of Chicago Medicine Northwest Indiana

Crown Point, Indiana, United States

Site Status RECRUITING

John's Hopkins Hospital

Baltimore, Maryland, United States

Site Status RECRUITING

Montefiore Einstein Medical Center

The Bronx, New York, United States

Site Status RECRUITING

UNC Chapel Hill

Chapel Hill, North Carolina, United States

Site Status RECRUITING

Abramson Cancer Center of the University of Pennsylvania

Philadelphia, Pennsylvania, United States

Site Status RECRUITING

University of Pittsburgh Medical Center

Pittsburgh, Pennsylvania, United States

Site Status RECRUITING

UPMC Hillman CC

Pittsburgh, Pennsylvania, United States

Site Status RECRUITING

Smilow Cancer Hospital Care Center

Westerly, Rhode Island, United States

Site Status RECRUITING

Ben Taub General Hospital

Houston, Texas, United States

Site Status RECRUITING

MD Anderson Cancer center

Houston, Texas, United States

Site Status RECRUITING

Baylor College of Medicine/Dan L Duncan Comprehensive Cancer Center

Houston, Texas, United States

Site Status RECRUITING

MD Anderson Cancer Center

Houston, Texas, United States

Site Status RECRUITING

MD Anderson Cancer center

League City, Texas, United States

Site Status RECRUITING

MD Anderson Cancer Center

Sugar Land, Texas, United States

Site Status RECRUITING

MD Anderson Cancer center

Woodland, Texas, United States

Site Status RECRUITING

University of Washington - Fred Hutchinson Cancer Center

Seattle, Washington, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Angela DeMichele, MD

Role: CONTACT

215-908-2599

Facility Contacts

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Emma Brophy

Role: primary

Carl Brown

Role: primary

Carl Brown

Role: primary

Carl Brown

Role: primary

Carl Brown

Role: primary

Carl Brown

Role: primary

Carl Brown

Role: primary

Carl Brown

Role: primary

Carl Brown, MHS

Role: primary

Carl Brown

Role: primary

Carl Brown

Role: primary

Carl Brown

Role: primary

Carl Brown

Role: primary

Carl Brown

Role: primary

Antonella Novielli

Role: primary

Antonella Novielli

Role: primary

Stephanie Grunwald

Role: primary

Vhenyse Encarnacion

Role: primary

Vhenyse Encarnacion

Role: primary

Vhenyse Encarnacion

Role: primary

Vhenyse Encarnacion

Role: primary

Jessica Jelinek

Role: primary

Alisia Laird

Role: primary

Mireille Leone

Role: primary

Angela DeMichele, MD

Role: primary

855-216-0098

AJ Jackson

Role: primary

AJ Jackson

Role: primary

Carl Brown

Role: primary

Emily Mowatt

Role: primary

Cynthia Tamez

Role: primary

Emily Mowatt

Role: primary

Cynthia Tamez

Role: primary

Cynthia Tamez

Role: primary

Cynthia Tamez

Role: primary

Cynthia Tamez

Role: primary

Emma Jackson

Role: primary

Provided Documents

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Document Type: Study Protocol and Statistical Analysis Plan

View Document

Document Type: Informed Consent Form

View Document

Other Identifiers

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855924

Identifier Type: OTHER

Identifier Source: secondary_id

UPCC 09124

Identifier Type: -

Identifier Source: org_study_id

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