Intestinal Microbiota and Their Antibiotic Resistance Genes of ICU Health Care Workers

NCT ID: NCT06524765

Last Updated: 2024-12-10

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 78 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2024-08-04
• Study Completion Date: 2024-10-16

Brief Summary

In this study, metagenomic sequencing (10G) results of stool samples from health care workers in ICU and non-medical professionals were compared to observe whether there are significant differences in community diversity, structure and function of intestinal microbiota and whether there are drug resistance genes carried by intestinal microbes, so as to determine whether long-term exposure to multi-pathogen environment in ICU has an impact on intestinal microbiota.

Detailed Description

The infection rate of multidrug-resistant bacteria in critically ill patients in intensive care unit (ICU) is high, resulting in high mortality, prolonged hospitalization, and becoming a source of pathogen transmission. The front-line medical staff engaged in ICU are constantly in contact with patients and exposed to the intensive care unit (ICU) environment, and this high-risk contact and exposure makes many pathogenic microorganisms and their drug-resistance genes become part of the intestinal microbiota of ICU medical staff and are carried. So far, there have been no previous reports on the characteristics of intestinal microbiome and the drug resistance genes carried by ICU staff. Therefore, in this study, metagenomic sequencing (10G) results of stool samples from medical staff and non-medical professionals in ICU were compared to observe whether there are significant differences in community diversity, structure and function of intestinal microbiota and whether there are drug resistance genes carried by intestinal microbes, so as to determine whether long-term exposure to multi-pathogen environment in ICU has an impact on intestinal microbiota.

Conditions

Intensive Care Unit

Keywords

intestinal microbiota; metagenomic sequencing

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: CROSS_SECTIONAL

Study Groups

ICU health care workers (exposed group)

The infection rate of multidrug-resistant bacteria in critically ill patients in intensive care unit (ICU) is high, the mortality rate of infected patients is high, which becomes the source of pathogen transmission. The front-line medical staff engaged in ICU are constantly in contact with patients and exposed to the intensive care unit (ICU) environment full of pathogens and even drug-resistant bacteria, and this high-risk contact and exposure makes many pathogenic microorganisms and their drug-resistant genes become part of the intestinal microbiota of ICU medical staff and are carried. Therefore, the exposure factor of the exposure group in this study was ICU environment.

Living conditions

Intervention Type: OTHER

There are many patients with multi-resistant bacterial infections in intensive care units (ICU), making it possible for a great deal of these pathogens to exist in the ICU environment. The exposure factors in this study were ICU environment or daily living environment.

Non-medical professionals (non-exposed group)

This group is a non-exposed group, so the exposed factor is the daily living environment.

No interventions assigned to this group

Interventions

Living conditions

There are many patients with multi-resistant bacterial infections in intensive care units (ICU), making it possible for a great deal of these pathogens to exist in the ICU environment. The exposure factors in this study were ICU environment or daily living environment.

Intervention Type: OTHER

Eligibility Criteria

Exclusion Criteria

1. Active gastrointestinal infection or chronic gastrointestinal disease;

2. Significant dietary changes or significant weight fluctuations (gain or loss > 5 kg) within 3 months;

3. Antibiotics, proton pump inhibitors or prebiotics, probiotics , including yogurt, have been used in the past 4 weeks;

4. Drinking within the past week;

5. Those who have recently been treated with high-risk immunosuppressive or cytotoxic drugs, such as medium-high dose steroid hormones (20 mg/day or higher) for more than 4 weeks;

6. History of major gastrointestinal surgery;

7. Chronic diseases that may affect the gut microbiota;

8. Congenital or acquired immunodeficiency disease;

9. Do not agree to provide information about their diet, exercise and other lifestyle factors that may affect the composition of their microbiome;

10. Pregnant or lactating women;

11. Participate in other clinical studies at the time of enrollment or within 3 months before enrollment;

12. Do not agree to sign a written informed consent.

• Minimum Eligible Age: 25 Years
• Maximum Eligible Age: 40 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

OTHER

Sponsor Role: lead

Responsible Party

Jiancheng Zhang

Prof. Jiancheng Zhang

Responsibility Role: PRINCIPAL_INVESTIGATOR

Locations

Department of Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

Wuhan, Hubei, China

Countries

China

Other Identifiers

zjc202403

Identifier Type: -

Identifier Source: org_study_id