Differentiation of Benign and Malignant Pulmonary Nodules by Volatile Organic Compounds in Human Exhaled Breath

NCT ID: NCT06518655

Last Updated: 2025-12-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

Get a concise snapshot of the trial, including recruitment status, study phase, enrollment targets, and key timeline milestones.

Recruitment Status

RECRUITING

Total Enrollment

3000 participants

Study Classification

OBSERVATIONAL

Study Start Date

2024-06-30

Study Completion Date

2027-06-30

Brief Summary

Review the sponsor-provided synopsis that highlights what the study is about and why it is being conducted.

The goal of this observational study is to develop an advanced expiratory algorithm model utilizing exhaled breath volatile organic compound (VOC) markers. This model aims to accurately differentiate benign from malignant nodules in individuals harboring pulmonary nodules. The primary objectives it strives to accomplish are:

1. To assess the diagnostic accuracy of an exhaled breath VOC-assisted diagnostic artificial intelligence (AI) model in distinguishing benign and malignant pulmonary nodules.
2. To evaluate the diagnostic effectiveness of an AI model that employs exhaled breath VOC biomakers to identify specific types of malignant nodules, including lung adenocarcinoma, lung squamous cell carcinoma, and small cell lung cancer.
3. To explore and identify key characteristic VOCs combinations that are associated with EGFR site mutations in malignant nodules, further modeling and evaluating the classification performance.

By utilizing this comprehensive approach, the study hopes to contribute significantly to early detection and accurate classification of pulmonary nodules, ultimately leading to improved patient care and treatment outcomes.

Detailed Description

Dive into the extended narrative that explains the scientific background, objectives, and procedures in greater depth.

This is a prospective, cross-sectional, and observational cohort study aiming at recruiting 3000 participants with pulmonary nodules ranging from 5 to 30 mm in diameter. Prior to invasive surgery, exhaled breath samples will be collected from these participants and analyzed using Gas chromatography-mass spectrometry(GC-MS) and micro Gas Chromatography-photoionisation detector (μGC-PID) system. Following the acquisition of μGC-PID results, a comprehensive evaluation of the diagnostic performance of VOC biomakers distinguishing between benign and malignant pulmonary nodules will be conducted, leveraging histopathological findings, CT examination data, and clinical data.

Conditions

See the medical conditions and disease areas that this research is targeting or investigating.

Pulmonary Nodules, Multiple Pulmonary Nodules, Solitary Lung Cancer

Keywords

Explore important study keywords that can help with search, categorization, and topic discovery.

Pulmonary Nodules Lung Cancer Volatile Organic Compounds Human Exhaled Breath

Study Design

Understand how the trial is structured, including allocation methods, masking strategies, primary purpose, and other design elements.

Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

Review each arm or cohort in the study, along with the interventions and objectives associated with them.

Pulmonary Nodules

Pre-surgery adult patients with pulmonary nodule found by CT scan.

Gas chromatography-mass spectrometry(GC-MS) and micro Gas Chromatography-photoionisation detector (μGC-PID) system

Intervention Type OTHER

Detection of volatile organic compound molecules in human exhaled breath by GC-MS and μGC-PID

Interventions

Learn about the drugs, procedures, or behavioral strategies being tested and how they are applied within this trial.

Gas chromatography-mass spectrometry(GC-MS) and micro Gas Chromatography-photoionisation detector (μGC-PID) system

Detection of volatile organic compound molecules in human exhaled breath by GC-MS and μGC-PID

Intervention Type OTHER

Eligibility Criteria

Check the participation requirements, including inclusion and exclusion rules, age limits, and whether healthy volunteers are accepted.

Inclusion Criteria

* 18-80 years old;
* Pulmonary nodules were detected through low-dose spiral CT, chest CT conventional scan, or high-resolution thin-layer CT examination, with a maximum diameter of 5-30 mm, including solid nodules and ground glass nodules;
* Patients require pulmonary nodule resection to define the type of nodule pathology;
* The Patients have not yet used any drugs for tumor treatment;
* Patients and/or family members are able to understand the research protocol and are willing to participate in this study, providing written informed consent.

Exclusion Criteria

* The maximum diameter of pulmonary nodules is greater than 30 mm;
* Patients are unable to determine the pathological diagnosis of pulmonary nodules after surgical resection or biopsy;
* Patients with recurrent lung cancer;
* Patients who have undergone lung transplantation or lobectomy;
* Individuals who currently or have a history of malignant tumors;
* Patients in the acute phase of inflammation or in need of intensive care in the above selected disease groups;
* Individuals with severe liver and kidney dysfunction;
* Mental illness patients (such as severe dementia, schizophrenia, severe depression, manic depressive psychosis, etc.);
* Confirmed HIV patients;
* Pregnant or lactating women;
* Patients or family members are unable to understand the conditions and objectives of this study.
* The patient is unwilling or unable to personally sign the informed consent form.
Minimum Eligible Age

18 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

Meet the organizations funding or collaborating on the study and learn about their roles.

The First Affiliated Hospital of Guangzhou Medical University

OTHER

Sponsor Role collaborator

First People's Hospital of Foshan

OTHER

Sponsor Role collaborator

Sichuan Cancer Hospital and Research Institute

OTHER

Sponsor Role collaborator

Liwan District Central Hospital

UNKNOWN

Sponsor Role collaborator

Shanghai Chest Hospital

OTHER

Sponsor Role collaborator

Peking Union Medical College Hospital

OTHER

Sponsor Role collaborator

Guangzhou Development Zone Hospital

UNKNOWN

Sponsor Role collaborator

Huangpu District Hongshan Street Community Health Service Center

UNKNOWN

Sponsor Role collaborator

Huangpu District Chinese Medicine Hospital

UNKNOWN

Sponsor Role collaborator

Fifth Affiliated Hospital of Guangzhou Medical University

OTHER

Sponsor Role collaborator

Huangpu District Jiufo Street Community Health Service Center

UNKNOWN

Sponsor Role collaborator

Huangpu District Xinlong Town Central Hospital

UNKNOWN

Sponsor Role collaborator

Huangpu District Yonghe Street Community Health Service Center

UNKNOWN

Sponsor Role collaborator

Huangpu District Lianhe Street Second Community Health Service Center

UNKNOWN

Sponsor Role collaborator

Renmin Hospital of Wuhan University

OTHER

Sponsor Role collaborator

ChromX Health

INDUSTRY

Sponsor Role lead

Responsible Party

Identify the individual or organization who holds primary responsibility for the study information submitted to regulators.

Responsibility Role SPONSOR

Principal Investigators

Learn about the lead researchers overseeing the trial and their institutional affiliations.

Jianxing He, MD

Role: STUDY_CHAIR

The First Affiliated Hospital of Guangzhou Medical University

Locations

Explore where the study is taking place and check the recruitment status at each participating site.

Peking Union Medical College Hospital

Beijing, Beijing Municipality, China

Site Status RECRUITING

First People's Hospital of Foshan

Foshan, Guangdong, China

Site Status RECRUITING

The First Affiliated Hospital of Guangzhou Medical University

Guangzhou, Guangdong, China

Site Status RECRUITING

Liwan District Central Hospital

Guangzhou, Guangdong, China

Site Status RECRUITING

Guangzhou Development Zone Hospital

Guangzhou, Guangdong, China

Site Status RECRUITING

Huangpu District Chinese Medicine Hospital

Guangzhou, Guangdong, China

Site Status RECRUITING

Huangpu District Hongshan Street Community Health Service Center

Guangzhou, Guangdong, China

Site Status RECRUITING

Huangpu District Jiufo Street Community Health Service Center

Guangzhou, Guangdong, China

Site Status RECRUITING

Huangpu District Lianhe Street Second Community Health Service Center

Guangzhou, Guangdong, China

Site Status RECRUITING

Huangpu District Xinlong Town Central Hospital

Guangzhou, Guangdong, China

Site Status RECRUITING

Huangpu District Yonghe Street Community Health Service Center

Guangzhou, Guangdong, China

Site Status RECRUITING

The Fifth Affiliated Hospital of Guangzhou Medical University

Guangzhou, Guangdong, China

Site Status RECRUITING

Renmin Hospital of Wuhan University

Wuhan, Hubei, China

Site Status RECRUITING

Shanghai Chest Hospital

Shanghai, Shanghai Municipality, China

Site Status RECRUITING

Sichuan Cancer Hospital

Chengdu, Sichuan, China

Site Status RECRUITING

Countries

Review the countries where the study has at least one active or historical site.

China

Central Contacts

Reach out to these primary contacts for questions about participation or study logistics.

Hengrui Liang, MD

Role: CONTACT

Phone: +86 15625064712

Email: [email protected]

Facility Contacts

Find local site contact details for specific facilities participating in the trial.

Qian Wang, MD

Role: primary

Zhuxing Chen

Role: primary

Hengrui Liang, MD

Role: primary

Hengrui Liang, MD

Role: primary

Hengrui Liang, MD

Role: primary

Hengrui Liang, MD

Role: primary

Hengrui Liang, MD

Role: primary

Hengrui Liang, MD

Role: primary

Hengrui Liang, MD

Role: primary

Hengrui Liang, MD

Role: primary

Hengrui Liang, MD

Role: primary

Hengrui Liang, MD

Role: primary

Huiqing Lin, MD

Role: primary

Yanwei Zhang, MD

Role: primary

Bo Tian, MD

Role: primary

References

Explore related publications, articles, or registry entries linked to this study.

Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021 May;71(3):209-249. doi: 10.3322/caac.21660. Epub 2021 Feb 4.

Reference Type BACKGROUND
PMID: 33538338 (View on PubMed)

Xia C, Dong X, Li H, Cao M, Sun D, He S, Yang F, Yan X, Zhang S, Li N, Chen W. Cancer statistics in China and United States, 2022: profiles, trends, and determinants. Chin Med J (Engl). 2022 Feb 9;135(5):584-590. doi: 10.1097/CM9.0000000000002108.

Reference Type BACKGROUND
PMID: 35143424 (View on PubMed)

Miller KD, Siegel RL, Lin CC, Mariotto AB, Kramer JL, Rowland JH, Stein KD, Alteri R, Jemal A. Cancer treatment and survivorship statistics, 2016. CA Cancer J Clin. 2016 Jul;66(4):271-89. doi: 10.3322/caac.21349. Epub 2016 Jun 2.

Reference Type BACKGROUND
PMID: 27253694 (View on PubMed)

National Lung Screening Trial Research Team; Aberle DR, Adams AM, Berg CD, Black WC, Clapp JD, Fagerstrom RM, Gareen IF, Gatsonis C, Marcus PM, Sicks JD. Reduced lung-cancer mortality with low-dose computed tomographic screening. N Engl J Med. 2011 Aug 4;365(5):395-409. doi: 10.1056/NEJMoa1102873. Epub 2011 Jun 29.

Reference Type BACKGROUND
PMID: 21714641 (View on PubMed)

Shlomi D, Abud M, Liran O, Bar J, Gai-Mor N, Ilouze M, Onn A, Ben-Nun A, Haick H, Peled N. Detection of Lung Cancer and EGFR Mutation by Electronic Nose System. J Thorac Oncol. 2017 Oct;12(10):1544-1551. doi: 10.1016/j.jtho.2017.06.073. Epub 2017 Jul 12.

Reference Type BACKGROUND
PMID: 28709937 (View on PubMed)

van de Goor R, van Hooren M, Dingemans AM, Kremer B, Kross K. Training and Validating a Portable Electronic Nose for Lung Cancer Screening. J Thorac Oncol. 2018 May;13(5):676-681. doi: 10.1016/j.jtho.2018.01.024. Epub 2018 Feb 6.

Reference Type BACKGROUND
PMID: 29425703 (View on PubMed)

Hanna GB, Boshier PR, Markar SR, Romano A. Accuracy and Methodologic Challenges of Volatile Organic Compound-Based Exhaled Breath Tests for Cancer Diagnosis: A Systematic Review and Meta-analysis. JAMA Oncol. 2019 Jan 1;5(1):e182815. doi: 10.1001/jamaoncol.2018.2815. Epub 2019 Jan 10.

Reference Type BACKGROUND
PMID: 30128487 (View on PubMed)

Horvath I, Lazar Z, Gyulai N, Kollai M, Losonczy G. Exhaled biomarkers in lung cancer. Eur Respir J. 2009 Jul;34(1):261-75. doi: 10.1183/09031936.00142508.

Reference Type BACKGROUND
PMID: 19567608 (View on PubMed)

Mitsui T, Kondo T. Inadequacy of theoretical basis of breath methylated alkane contour for assessing oxidative stress. Clin Chim Acta. 2003 Jul 1;333(1):91; author reply 93-4. doi: 10.1016/s0009-8981(03)00173-6. No abstract available.

Reference Type BACKGROUND
PMID: 12809740 (View on PubMed)

Stone BG, Besse TJ, Duane WC, Evans CD, DeMaster EG. Effect of regulating cholesterol biosynthesis on breath isoprene excretion in men. Lipids. 1993 Aug;28(8):705-8. doi: 10.1007/BF02535990.

Reference Type BACKGROUND
PMID: 8377584 (View on PubMed)

Nakhleh MK, Amal H, Jeries R, Broza YY, Aboud M, Gharra A, Ivgi H, Khatib S, Badarneh S, Har-Shai L, Glass-Marmor L, Lejbkowicz I, Miller A, Badarny S, Winer R, Finberg J, Cohen-Kaminsky S, Perros F, Montani D, Girerd B, Garcia G, Simonneau G, Nakhoul F, Baram S, Salim R, Hakim M, Gruber M, Ronen O, Marshak T, Doweck I, Nativ O, Bahouth Z, Shi DY, Zhang W, Hua QL, Pan YY, Tao L, Liu H, Karban A, Koifman E, Rainis T, Skapars R, Sivins A, Ancans G, Liepniece-Karele I, Kikuste I, Lasina I, Tolmanis I, Johnson D, Millstone SZ, Fulton J, Wells JW, Wilf LH, Humbert M, Leja M, Peled N, Haick H. Diagnosis and Classification of 17 Diseases from 1404 Subjects via Pattern Analysis of Exhaled Molecules. ACS Nano. 2017 Jan 24;11(1):112-125. doi: 10.1021/acsnano.6b04930. Epub 2016 Dec 21.

Reference Type BACKGROUND
PMID: 28000444 (View on PubMed)

Zhou J, Huang ZA, Kumar U, Chen DDY. Review of recent developments in determining volatile organic compounds in exhaled breath as biomarkers for lung cancer diagnosis. Anal Chim Acta. 2017 Dec 15;996:1-9. doi: 10.1016/j.aca.2017.09.021. Epub 2017 Sep 13.

Reference Type BACKGROUND
PMID: 29137702 (View on PubMed)

Arasaradnam RP, Wicaksono A, O'Brien H, Kocher HM, Covington JA, Crnogorac-Jurcevic T. Noninvasive Diagnosis of Pancreatic Cancer Through Detection of Volatile Organic Compounds in Urine. Gastroenterology. 2018 Feb;154(3):485-487.e1. doi: 10.1053/j.gastro.2017.09.054. Epub 2017 Nov 10. No abstract available.

Reference Type BACKGROUND
PMID: 29129714 (View on PubMed)

Chan DK, Zakko L, Visrodia KH, Leggett CL, Lutzke LS, Clemens MA, Allen JD, Anderson MA, Wang KK. Breath Testing for Barrett's Esophagus Using Exhaled Volatile Organic Compound Profiling With an Electronic Nose Device. Gastroenterology. 2017 Jan;152(1):24-26. doi: 10.1053/j.gastro.2016.11.001. Epub 2016 Nov 5. No abstract available.

Reference Type BACKGROUND
PMID: 27825962 (View on PubMed)

Gordon SM, Szidon JP, Krotoszynski BK, Gibbons RD, O'Neill HJ. Volatile organic compounds in exhaled air from patients with lung cancer. Clin Chem. 1985 Aug;31(8):1278-82.

Reference Type BACKGROUND
PMID: 4017231 (View on PubMed)

Corradi M, Pesci A, Casana R, Alinovi R, Goldoni M, Vettori MV, Cuomo A. Nitrate in exhaled breath condensate of patients with different airway diseases. Nitric Oxide. 2003 Feb;8(1):26-30. doi: 10.1016/s1089-8603(02)00128-3.

Reference Type BACKGROUND
PMID: 12586538 (View on PubMed)

Bousamra M 2nd, Schumer E, Li M, Knipp RJ, Nantz MH, van Berkel V, Fu XA. Quantitative analysis of exhaled carbonyl compounds distinguishes benign from malignant pulmonary disease. J Thorac Cardiovasc Surg. 2014 Sep;148(3):1074-80; discussion 1080-1. doi: 10.1016/j.jtcvs.2014.06.006. Epub 2014 Jun 8.

Reference Type BACKGROUND
PMID: 25129599 (View on PubMed)

Phillips M, Altorki N, Austin JH, Cameron RB, Cataneo RN, Greenberg J, Kloss R, Maxfield RA, Munawar MI, Pass HI, Rashid A, Rom WN, Schmitt P. Prediction of lung cancer using volatile biomarkers in breath. Cancer Biomark. 2007;3(2):95-109. doi: 10.3233/cbm-2007-3204.

Reference Type BACKGROUND
PMID: 17522431 (View on PubMed)

Phillips M, Bauer TL, Pass HI. A volatile biomarker in breath predicts lung cancer and pulmonary nodules. J Breath Res. 2019 Jun 19;13(3):036013. doi: 10.1088/1752-7163/ab21aa.

Reference Type BACKGROUND
PMID: 31085817 (View on PubMed)

Phillips M, Cataneo RN, Greenberg J, Grodman R, Gunawardena R, Naidu A. Effect of oxygen on breath markers of oxidative stress. Eur Respir J. 2003 Jan;21(1):48-51. doi: 10.1183/09031936.02.00053402.

Reference Type BACKGROUND
PMID: 12570108 (View on PubMed)

Other Identifiers

Review additional registry numbers or institutional identifiers associated with this trial.

LCLN01

Identifier Type: -

Identifier Source: org_study_id