Trial Outcomes & Findings for Evaluation of the Efficacy and Safety of a Loop Stretchable Dental Floss (NCT NCT06515483)

NCT ID: NCT06515483

Last Updated: 2025-06-13

Results Overview

Dental plaque was identified using a disclosing solution and scored using the Turesky modification of the Quigley-Hein Plaque Index (PLI). The index score was scored between 0 and 5, with 0 = no plaque detected and 5= = Plaque covering 2/3 or more of the tooth's crown. The remaining values are based on the amount of plaque covering the tooth. The higher the score, the more plaque accumulated and the worse the outcome for the surface. Each tooth, except the third molars, will be scored at six sites: Mesio-Buccal, buccal, disto-buccal, mesio-lingual, lingual, and disto-lingual. The plaque score index for an individual is determined by adding all the individual scores and dividing the total score by the number of surfaces examined.

Recruitment status

COMPLETED

Study phase

NA

Target enrollment

64 participants

Primary outcome timeframe

Baseline and 30 days examination

Results posted on

2025-06-13

Participant Flow

Recruitment started on October 15 and ended on December 4th. Recruitment was conducted in the OHRI Clinic.

There were not significant events reported prior to participants enrollment.

Participant milestones

Participant milestones
Measure
Brushing and Flossing
32 subjects were instructed to clean their teeth daily using only the provided toothbrush with an assigned ADA-accepted fluoride dentifrice. Subjects in the experimental group brushed and flossed with the provided stretchable loop floss at least twice a day. To assess compliance, each subject was asked to complete a diary every time they brushed or flossed. On day 15 (+/- 2) day of product use, subjects received a phone call from the study coordinator or designee to assess brushing/flossing compliance. At day 30 (+/- 2), study hygiene and gum chewing restrictions as well as eating restriction applied, and the efficacy assessments were repeated, subjects were dismissed, and the study was finished.
Brushing Only
32 subjects were instructed to clean their teeth daily using only the provided toothbrush with an assigned ADA-accepted fluoride dentifrice. Subjects assigned to the control group brushed their teeth at least twice a day only. On day 15 (+/- 2) day of product use, subjects received a phone call from the study coordinator or designee to assess brushing/flossing compliance. At day 30 (+/- 2), study hygiene and gum chewing restrictions as well as eating restriction applied, and the efficacy assessments were repeated, subjects were dismissed, and the study was finished.
Overall Study
STARTED
32
32
Overall Study
COMPLETED
31
32
Overall Study
NOT COMPLETED
1
0

Reasons for withdrawal

Reasons for withdrawal
Measure
Brushing and Flossing
32 subjects were instructed to clean their teeth daily using only the provided toothbrush with an assigned ADA-accepted fluoride dentifrice. Subjects in the experimental group brushed and flossed with the provided stretchable loop floss at least twice a day. To assess compliance, each subject was asked to complete a diary every time they brushed or flossed. On day 15 (+/- 2) day of product use, subjects received a phone call from the study coordinator or designee to assess brushing/flossing compliance. At day 30 (+/- 2), study hygiene and gum chewing restrictions as well as eating restriction applied, and the efficacy assessments were repeated, subjects were dismissed, and the study was finished.
Brushing Only
32 subjects were instructed to clean their teeth daily using only the provided toothbrush with an assigned ADA-accepted fluoride dentifrice. Subjects assigned to the control group brushed their teeth at least twice a day only. On day 15 (+/- 2) day of product use, subjects received a phone call from the study coordinator or designee to assess brushing/flossing compliance. At day 30 (+/- 2), study hygiene and gum chewing restrictions as well as eating restriction applied, and the efficacy assessments were repeated, subjects were dismissed, and the study was finished.
Overall Study
Subject withdraw due to family issues
1
0

Baseline Characteristics

Race and Ethnicity were not collected from any participant.

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Brushing and Flossing
n=32 Participants
Brushing and Flossing Brushing and Flossing of teeth: 32 subjects will be instructed to clean their teeth daily using only the provided toothbrush with an assigned ADA-accepted fluoride dentifrice. Subjects in the experimental group will brush and floss with the provided stretchable loop floss at least twice a day. To assess compliance, each subject will be asked to fill out a diary every time they brush/floss. On day 15 (+/- 2) day of product use, subjects will receive a phone call from the study coordinator or designee to assess brushing/flossing compliance. At day 30 (+/- 2), study hygiene and gum chewing restrictions as well as eating restriction will apply, and the efficacy assessments will be repeated, subjects will be dismissed, and the study will finish.
Brushing Only
n=32 Participants
Brushing only Brushing Only: 32 subjects will be instructed to clean their teeth daily using only the provided toothbrush with an assigned ADA-accepted fluoride dentifrice. Subjects assigned to the control group will brush their teeth at least twice a day only. On day 15 (+/- 2) day of product use, subjects will receive a phone call from the study coordinator or designee to assess brushing/flossing compliance. At day 30 (+/- 2), study hygiene and gum chewing restrictions as well as eating restriction will apply, and the efficacy assessments will be repeated, subjects will be dismissed, and the study will finish.
Total
n=64 Participants
Total of all reporting groups
Age, Categorical
<=18 years
0 Participants
n=32 Participants
0 Participants
n=32 Participants
0 Participants
n=64 Participants
Age, Categorical
Between 18 and 65 years
32 Participants
n=32 Participants
32 Participants
n=32 Participants
64 Participants
n=64 Participants
Age, Categorical
>=65 years
0 Participants
n=32 Participants
0 Participants
n=32 Participants
0 Participants
n=64 Participants
Age, Continuous
43.225 Years
n=32 Participants
42.36 Years
n=32 Participants
42.792 Years
n=64 Participants
Sex: Female, Male
Female
20 Participants
n=32 Participants
19 Participants
n=32 Participants
39 Participants
n=64 Participants
Sex: Female, Male
Male
12 Participants
n=32 Participants
13 Participants
n=32 Participants
25 Participants
n=64 Participants
Race and Ethnicity Not Collected
0 Participants
Race and Ethnicity were not collected from any participant.
Region of Enrollment
United States
32 Participants
n=32 Participants
32 Participants
n=32 Participants
64 Participants
n=64 Participants
The Modified Gingival Index by Lobene
2.335 Index
STANDARD_DEVIATION 0.047 • n=32 Participants
2.257 Index
STANDARD_DEVIATION 0.042 • n=32 Participants
2.296 Index
STANDARD_DEVIATION 0.032 • n=64 Participants
Bleeding on Probing
25.18 Percentage of bleeding sites
STANDARD_DEVIATION 2.27 • n=32 Participants
24.87 Percentage of bleeding sites
STANDARD_DEVIATION 2.35 • n=32 Participants
25.03 Percentage of bleeding sites
STANDARD_DEVIATION 1.62 • n=64 Participants
Turesky Dental Plaque Index
4.026 Index
STANDARD_DEVIATION 0.063 • n=32 Participants
3.947 Index
STANDARD_DEVIATION 0.061 • n=32 Participants
3.986 Index
STANDARD_DEVIATION 0.044 • n=64 Participants

PRIMARY outcome

Timeframe: Baseline and 30 days examination

Population: One patient who did not complete the 30-day follow-up examination was not included in the efficacy analysis.

Dental plaque was identified using a disclosing solution and scored using the Turesky modification of the Quigley-Hein Plaque Index (PLI). The index score was scored between 0 and 5, with 0 = no plaque detected and 5= = Plaque covering 2/3 or more of the tooth's crown. The remaining values are based on the amount of plaque covering the tooth. The higher the score, the more plaque accumulated and the worse the outcome for the surface. Each tooth, except the third molars, will be scored at six sites: Mesio-Buccal, buccal, disto-buccal, mesio-lingual, lingual, and disto-lingual. The plaque score index for an individual is determined by adding all the individual scores and dividing the total score by the number of surfaces examined.

Outcome measures

Outcome measures
Measure
Brushing Only
n=32 Participants
Brushing Only: 32 subjects will be instructed to clean their teeth daily using only the provided toothbrush with an assigned ADA-accepted fluoride dentifrice. Subjects assigned to the control group will brush their teeth at least twice a day only. On day 15 (+/- 2) day of product use, subjects will receive a phone call from the study coordinator or designee to assess brushing/flossing compliance. At day 30 (+/- 2), study hygiene and gum chewing restrictions as well as eating restriction will apply, and the efficacy assessments will be repeated, subjects will be dismissed, and the study will finish.
Brushing and Flossing
n=31 Participants
Brushing and Flossing of teeth: 32 subjects will be instructed to clean their teeth daily using only the provided toothbrush with an assigned ADA-accepted fluoride dentifrice. Subjects in the experimental group will brush and floss with the provided stretchable loop floss at least twice a day. To assess compliance, each subject will be asked to fill out a diary every time they brush/floss. On day 15 (+/- 2) day of product use, subjects will receive a phone call from the study coordinator or designee to assess brushing/flossing compliance. At day 30 (+/- 2), study hygiene and gum chewing restrictions as well as eating restriction will apply, and the efficacy assessments will be repeated, subjects will be dismissed, and the study will finish.
Dental Plaque Assessment
Baseline
3.947 Index
Standard Deviation 0.061
4.026 Index
Standard Deviation 0.063
Dental Plaque Assessment
30 days
3.892 Index
Standard Deviation 0.047
3.856 Index
Standard Deviation 0.036
Dental Plaque Assessment
PLI change from baseline to 30 days
-0.055 Index
Standard Deviation 0.057
-0.170 Index
Standard Deviation 0.046

PRIMARY outcome

Timeframe: Baseline and 30 days examination

Population: One patient who did not complete the 30-day follow-up examination was not included in the efficacy analysis.

The Lobene-modified gingival index (MGI) will be used. This is a non-invasive index that assesses the health of the gums based on their visual clinical appearance. Each tooth will be evaluated visually on six surfaces (Mesio-buccal, buccal, distobuccal, mesio-lingual, lingual, and disto-lingual). Each surface will be scored based on the inflammatory visual changes, severity, and extent. For the MGI calculation, all the values for each tooth will be added and divided by the number of surfaces examined. Scores range from 0 to 4. A score of 0 will represent normal. A score of 1 and 2 will represent mild inflammation, a score of 3 indicates moderate inflammation and a score of 4 indicates severe inflammation). A single, trained examiner will complete all the examinations.

Outcome measures

Outcome measures
Measure
Brushing Only
n=32 Participants
Brushing Only: 32 subjects will be instructed to clean their teeth daily using only the provided toothbrush with an assigned ADA-accepted fluoride dentifrice. Subjects assigned to the control group will brush their teeth at least twice a day only. On day 15 (+/- 2) day of product use, subjects will receive a phone call from the study coordinator or designee to assess brushing/flossing compliance. At day 30 (+/- 2), study hygiene and gum chewing restrictions as well as eating restriction will apply, and the efficacy assessments will be repeated, subjects will be dismissed, and the study will finish.
Brushing and Flossing
n=31 Participants
Brushing and Flossing of teeth: 32 subjects will be instructed to clean their teeth daily using only the provided toothbrush with an assigned ADA-accepted fluoride dentifrice. Subjects in the experimental group will brush and floss with the provided stretchable loop floss at least twice a day. To assess compliance, each subject will be asked to fill out a diary every time they brush/floss. On day 15 (+/- 2) day of product use, subjects will receive a phone call from the study coordinator or designee to assess brushing/flossing compliance. At day 30 (+/- 2), study hygiene and gum chewing restrictions as well as eating restriction will apply, and the efficacy assessments will be repeated, subjects will be dismissed, and the study will finish.
Gingivitis Assessment
Baseline
2.257 Index
Standard Deviation 0.042
2.335 Index
Standard Deviation 0.047
Gingivitis Assessment
30 days
2.312 Index
Standard Deviation 0.033
2.227 Index
Standard Deviation 0.049
Gingivitis Assessment
MGI change from baseline to 30 days
0.055 Index
Standard Deviation 0.030
-0.108 Index
Standard Deviation 0.031

SECONDARY outcome

Timeframe: Baseline and 30 days examination

Population: One patient who did not complete the 30-day follow-up examination was not included in the efficacy analysis.

Bleeding on probing will be assessed at six surfaces (Mesio-buccal, buccal, distobuccal, mesio-lingual, lingual, and disto-lingual). A periodontal probe with a rounded tip 0.5mm in diameter will be gently inserted into the gingival crevice to a depth of approximately 2 mm and run at an angle of roughly 60° about the longitudinal axis of the tooth, except for third molars if present. BOPBOMP will be read up to 30 seconds after probing. The surfaces would be scored as negative (0=no BOPBOMP) or positive (1=BOPBOMP). The whole-mouth BOPBOMP score will be calculated as the percentage of bleeding sites, will be determined by adding up the number of bleeding sites, dividing this number by the total number of probing sites, and multiplying by 100. Percentage of Bleeding sites between 20% 40 are considered moderate. Below 20% mild and higher than 40%severe.

Outcome measures

Outcome measures
Measure
Brushing Only
n=32 Participants
Brushing Only: 32 subjects will be instructed to clean their teeth daily using only the provided toothbrush with an assigned ADA-accepted fluoride dentifrice. Subjects assigned to the control group will brush their teeth at least twice a day only. On day 15 (+/- 2) day of product use, subjects will receive a phone call from the study coordinator or designee to assess brushing/flossing compliance. At day 30 (+/- 2), study hygiene and gum chewing restrictions as well as eating restriction will apply, and the efficacy assessments will be repeated, subjects will be dismissed, and the study will finish.
Brushing and Flossing
n=31 Participants
Brushing and Flossing of teeth: 32 subjects will be instructed to clean their teeth daily using only the provided toothbrush with an assigned ADA-accepted fluoride dentifrice. Subjects in the experimental group will brush and floss with the provided stretchable loop floss at least twice a day. To assess compliance, each subject will be asked to fill out a diary every time they brush/floss. On day 15 (+/- 2) day of product use, subjects will receive a phone call from the study coordinator or designee to assess brushing/flossing compliance. At day 30 (+/- 2), study hygiene and gum chewing restrictions as well as eating restriction will apply, and the efficacy assessments will be repeated, subjects will be dismissed, and the study will finish.
Bleeding on Marginal Probing (BOMP)
Baseline
24.87 Percentage of bleeding sites
Standard Deviation 2.35
25.18 Percentage of bleeding sites
Standard Deviation 2.27
Bleeding on Marginal Probing (BOMP)
30 days
25.63 Percentage of bleeding sites
Standard Deviation 2.62
17.41 Percentage of bleeding sites
Standard Deviation 3.20
Bleeding on Marginal Probing (BOMP)
BOMP change from baseline to 30 days
0.76 Percentage of bleeding sites
Standard Deviation 2.59
-7.77 Percentage of bleeding sites
Standard Deviation 2.91

Adverse Events

Arm 1

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Arm 2

Serious events: 0 serious events
Other events: 0 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Adverse event data not reported

Additional Information

Steven Kayser, President

Loops, LLC

Phone: 360-366-3009

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place